Imaging flow cytometry for morphologic and phenotypic characterization of rare circulating endothelial cells

Cytometry Part B - Clinical Cytometry

Volumn 84, Issue 6, 2013, Pages 379-389

  Samsel, Leigh ^a   Dagur, Pradeep K ^a   Raghavachari, Nalini ^a   Seamon, Catherine ^a   Kato, Gregory J ^a   McCoy Jr , J Philip ^a  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

circulating endothelial cells; imaging cytometry; rare event cytometry

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; CD146 ANTIGEN; CD3 ANTIGEN; CD45 ANTIGEN; FLUOROCHROME;

APOPTOSIS; ARTICLE; AUTOPHAGY; CAPPING PHENOMENON; CELL LINEAGE; CELL SELECTION; CELL STRUCTURE; CHANNEL GATING; CIRCULATING ENDOTHELIAL CELL; CONTROLLED STUDY; ENDOTHELIUM; ENDOTHELIUM CELL; ERYTHROCYTE; FLOW CYTOMETRY; GENOMICS; HUMAN; HUMAN CELL; MOLECULAR IMAGING; MORPHOLOGY; PERIPHERAL CIRCULATION; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; TREATMENT RESPONSE;

CIRCULATING ENDOTHELIAL CELLS; IMAGING CYTOMETRY; RARE EVENT CYTOMETRY;

ANTIGENS, CD146; CELL COUNT; CELL LINEAGE; DIAGNOSTIC IMAGING; ENDOTHELIAL CELLS; ERYTHROCYTES; FLOW CYTOMETRY; HUMANS;

EID: 84887498634     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.21088     Document Type: Article

References (15)

1. Khan SS, Solomon MA, McCoy JP Jr,. Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry. Cytometry B Clin Cytom 2005; 64B: 1-8.
2. Blann AD, Woywodt A, Bertolini F, Bull TM, Buyon JP, Clancy RM, Haubitz M, Hebbel RP, Lip GY, Mancuso P, Sampol J, Solovey A, Dignat-George F,. Circulating endothelial cells. Biomarker of vascular disease. Thromb Haemost 2005; 93: 228-235.
3. Goon PK, Boos CJ, Stonelake PS, Blann AD, Lip GY,. Detection and quantification of mature circulating endothelial cells using flow cytometry and immunomagnetic beads: A methodological comparison. Thromb Haemost 2006; 96: 45-52.
4. Bull TM, Golpon H, Hebbel RP, Solovey A, Cool CD, Tuder RM, Geraci MW, Voelkel NF,. Circulating endothelial cells in pulmonary hypertension. Thromb Haemost 2003; 90: 698-703.
5. Rowand JL, Martin G, Doyle GV, Miller MC, Pierce MS, Connelly MC, Rao C, Terstappen LW,. Endothelial cells in peripheral blood of healthy subjects and patients with metastatic carcinomas. Cytometry A 2007; 71: 105-113.
6. Basiji DA, Ortyn WE, Liang L, Venkatachalam V, Morrissey P,. Cellular image analysis and imaging by flow cytometry. Clin Lab Med 2007: 27: 653-670.
7. Mancuso P, Antoniotti P, Quarna J, Calleri A, Rabascio C, Tacchetti C, Braidotti P, Wu HK, Zurita AJ, Saronni L, Cheng JB, Shalinsky DR, Heymach JV, Bertolini F,. Validation of a standardized method for enumerating circulating endothelial cells and progenitors: flow cytometry and molecular and ultrastructural analyses. Clin Cancer Res 2009; 15: 267-273.
8. Smirnov DA, Foulk BW, Doyle GV, Connelly MC, Terstappen LW, O'Hara SM,. Global gene expression profiling of circulating endothelial cells in patients with metastatic carcinomas. Cancer Res 2006; 66: 2918-2922.
9. Rigolin GM, Maffei R, Rizzotto L, Ciccone M, Sofritti O, Daghia G, Cibien F, Cavazzini F, Marasca R, Cuneo A,. Circulating endothelial cells in patients with chronic lymphocytic leukemia: Clinical-prognostic and biologic significance. Cancer 2010; 116: 1926-1937.
10. Livak KJ, Schmittgen TD,. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001; 25: 402-408.
11. Pradhan KR, Mund JA, Johnson C, Vik TA, Ingram DA, Case J,. Ploychromatic flow cytometry identifies novel subsets of circulating cells with angiogenic potential in pediatric solid tumors. Cytometry Part B 2011; 80B: 335-338.
12. Versaevel M, Grevesse T, and, Gabriele S,. Spatial coordination between cell and nuclear shape within micropatterned endothelial cells. Nature Commun 2012; 3: 671.
13. Du J, Teng R-J, Guan T, Eis A, Kaul S, Konduri G, Shi Y,. Role of autophagy in angiogenesis in aortic endothelial cells. Am J Physiol Cell Physiol 2012; 302: 383-391.
14. Singh N, Van Craeyveld E, Tjwa M, Ciarka A, Emmerechts J, Droogne W, Gordts SC, Carlier V, Jacobs F, Fieuws S, Vanhaecke J, Van Cleemput J, De Geest B,. Circulating apoptotic endothelial cells and apoptotic endothelial microparticles independently predict the presence of cardiac allograft vasculopathy. J Am Coll Cardiol 2012; 60: 324-331.
15. Dagur PK, Biancotto A, Wei L, Sen N, Yao M, Strober W, Nussenblatt RB, McCoy JP,. MCAM-expressing CD4+ T cells in peripheral blood secrete IL17A and are significantly elevated in patients with inflammatory autoimmune diseases. J Autoimmunity 2011; 37: 319-327.