Free fatty acid secretion by an engineered strain of Escherichia coli

Biotechnology Letters

Volumn 35, Issue 12, 2013, Pages 2099-2103

  Meng, Xin ^a   Shang, Hongli ^a   Zheng, Yi ^a   Zhang, Zhen ^a  

^a JINZHOU MEDICAL UNIVERSITY   (China)

Author keywords

Biofuels; Escherichia coli; Fatty acid secretion; Free fatty acids; Lipids

Indexed keywords

ATP-BINDING CASSETTE TRANSPORTERS; CULTURE MEDIA; DIFFUSION MECHANISMS; ENGINEERED STRAINS; FREE FATTY ACID; OVER-EXPRESSION; RECOMBINANT STRAINS; THIOESTERASES;

BIOFUELS; ESCHERICHIA COLI; LIPIDS; PHYSIOLOGY;

FATTY ACIDS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ABC TRANSPORTER; ACYL COENZYME A SYNTHETASE, E COLI; ACYL-COENZYME A SYNTHETASE, E COLI; BACTERIAL PROTEIN; BIOFUEL; ESCHERICHIA COLI PROTEIN; FADL PROTEIN, E COLI; FATTY ACID; FATTY ACID TRANSPORTER; LONG CHAIN FATTY ACID COENZYME A LIGASE; MSBA PROTEIN, BACTERIA; OUTER MEMBRANE PROTEIN;

ARTICLE; ENZYMOLOGY; ESCHERICHIA COLI; GENE INACTIVATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; METHODOLOGY;

ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BIOFUELS; COENZYME A LIGASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FATTY ACID TRANSPORT PROTEINS; FATTY ACIDS, NONESTERIFIED; GENE KNOCKOUT TECHNIQUES; METABOLIC ENGINEERING;

EID: 84887322124     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-013-1305-4     Document Type: Article

References (14)

1. Cho H, Cronan JE (1995) Defective export of a periplasmic enzyme disrupts regulation of fatty acid synthesis. J Biol Chem 270: 4216-4219.
2. Clomburg J, Gonzalez R (2010) Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology. Appl Microbiol Biotechnol 86: 419-434.
3. Davis MS, Solbiati J, Cronan JE (2000) Overproduction of acetyl-CoA carboxylase activity increases the rate of fatty acid biosynthesis in Escherichia coli. J Biol Chem 275: 28593-28598.
4. Doerrler WT, Gibbons HS, Raetz CRH (2004) MsbA-dependent translocation of lipids across the inner membrane of Escherichia coli. J Biol Chem 279: 45102-45109.
5. Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226: 497-509.
6. Frazier CL, San Filippo J, Lambowitz AM, Mills DA (2003) Genetic manipulation of Lactococcus lactis by using targeted group II introns: generation of stable insertions without selection. Appl Environ Microbiol 69: 1121-1128.
7. Ghosh SK, Bhattacharjee A, Jha JK et al (2007) Characterization and clonging of a stearoyl/oleoyl specific fatty acyl-acyl carrier protein thioesterase from the seeds of Madhuca longifolia (latifolia). Plant Physiol Biochem 45: 887-897.
8. Lowry R, Tinsley I (1976) Rapid colorimetric determination of free fatty acids. J Am Oil Chem Soc 53: 470-472.
9. Nunn WD, Colburn RW, Black PN (1986) Transport of long-chain fatty acids in Escherichia coli. Evidence for role of fadL gene product as long-chain fatty acid receptor. J Biol Chem 261: 167-171.
10. Reyes C, Chang G (2005) Structure of the ABC transporter MsbA in complex with ADP vanadate and lipopolysaccharide. Science 308: 1028-1031.
11. Schaffer JE, Lodish HF (1994) Expression, cloning and characterization of a novel adipocyte long-chain fatty acid transport protein. Cell 79: 427-436.
12. Steen EJ, Kang Y, Bokinsky G et al (2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature 463: 559-562.
13. Voelker TA, Davies HM (1994) Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase. J Bacteriol 176: 7320-7327.
14. Weimar JD, DiRusso CC, Delio R, Black PN (2002) A synthetase in the transmembrane movementand activation of exogenous long-chain fatty acids. Amino acidresidues within the ATP/AMP signature motif of Escherichia coli. FadD are required for enzyme activity and fatty acid transport. J Biol Chem 277: 29369-29376.