Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay - A methodological overview

DNA Repair

Volumn 12, Issue 11, 2013, Pages 1007-1010

  Azqueta, Amaya ^a,b   Langie, Sabine A S ^c,e   Slyskova, Jana ^d   Collins, Andrew R ^b  

^a UNIVERSITY OF NAVARRA   (Spain)

^b UNIVERSITY OF OSLO   (Norway)

^c NEWCASTLE UNIVERSITY   (United Kingdom)

^d INSTITUTE OF EXPERIMENTAL BOTANY   (Czech Republic)

^e FLEMISH INSTITUTE FOR TECHNOLOGICAL RESEARCH VITO   (Belgium)

Author keywords

Base excision repair; Cell; Comet assay; Nucleotide excision repair; Protocols; Tissue

Indexed keywords

BIOMATERIAL; DNA BASE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE;

BLOOD CELL; CELL CULTURE; COMET ASSAY; DNA DAMAGE; DNA DETERMINATION; DNA REPAIR; DNA STRAND BREAKAGE; EXCISION REPAIR; HUMAN; IN VITRO STUDY; MAMMAL CELL; NONHUMAN; NOTE; PRIORITY JOURNAL;

ANIMALIA; MAMMALIA; ZIZIPHUS MAURITIANA;

BASE EXCISION REPAIR; CELL; COMET ASSAY; NUCLEOTIDE EXCISION REPAIR; PROTOCOLS; TISSUE;

ANIMALS; COMET ASSAY; DNA DAMAGE; DNA REPAIR; HUMANS;

EID: 84887183019     PISSN: 15687864     EISSN: 15687856     Source Type: Journal    
DOI: 10.1016/j.dnarep.2013.07.011     Document Type: Note

References (14)

1. Azqueta A., Collins A.R. The essential comet assay: a comprehensive guide to measuring DNA damage and repair. Arch. Toxicol. 2013, 87:949-968.
2. Ostling O., Johanson K.J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 1984, 123:291-298.
3. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 1988, 175:184-191.
4. Collins A.R., Dusinska M., Horvathova E., Munro E., Savio M., Stetina R. Inter-individual differences in repair of DNA base oxidation, measured in vitro with the comet assay. Mutagenesis 2001, 16:297-301.
5. Collins A.R., Fleming I.M., Gedik C.M. In vitro repair of oxidative and ultraviolet-induced DNA damage in supercoiled nucleoid DNA by human cell extract. Biochim. Biophys. Acta 1994, 1219:724-727.
6. Gaivao I., Piasek A., Brevik A., Shaposhnikov S., Collins A.R. Comet assay-based methods for measuring DNA repair in vitro; estimates of inter- and intra-individual variation. Cell Biol. Toxicol. 2009, 25:45-52.
7. Langie S.A., Knaapen A.M., Brauers K.J., van Berlo D., van Schooten F.J., Godschalk R.W. Development and validation of a modified comet assay to phenotypically assess nucleotide excision repair. Mutagenesis 2006, 21:153-158.
8. Langie S.A., Cameron K.M., Waldron K.J., Fletcher K.P., von Zglinicki T., Mathers J.C. Measuring DNA repair incision activity of mouse tissue extracts towards singlet oxygen-induced DNA damage: a comet-based in vitro repair assay. Mutagenesis 2011, 26:461-471.
9. Slyskova J., Korenkova V., Collins A.R., Prochazka P., Vodickova L., Svec J., Lipska L., Levy M., Schneiderova M., Liska V., Holubec L., Kumar R., Soucek P., Naccarati A., Vodicka P. Functional, genetic, and epigenetic aspects of base and nucleotide excision repair in colorectal carcinomas. Clin. Cancer Res. 2012, 18:5878-5887.
10. Langie S.A., Kowalczyk P., Tudek B., Zabielski R., Dziaman T., Olinski R., van Schooten F.J., Godschalk R.W. The effect of oxidative stress on nucleotide-excision repair in colon tissue of newborn piglets. Mutat. Res. 2010, 695:75-80.
11. Paz-Elizur T., Elinger D., Leitner-Dagan Y., Blumenstein S., Krupsky M., Berrebi A., Schechtman E., Livneh Z. Development of an enzymatic DNA repair assay for molecular epidemiology studies: distribution of OGG activity in healthy individuals. DNA Repair (Amst) 2007, 6:45-60.
12. Millau J.F., Raffin A.L., Caillat S., Claudet C., Arras G., Ugolin N., Douki T., Ravanat J.L., Breton J., Oddos T., Dumontet C., Sarasin A., Chevillard S., Favier A., Sauvaigo S. A microarray to measure repair of damaged plasmids by cell lysates. Lab Chip 2008, 8:1713-1722.
13. Collins A.R., Ferguson L.R. DNA repair as a biomarker. Mutat. Res. 2012, 736:2-4.
14. Collins A.R., Azqueta A. DNA repair as a biomarker in human biomonitoring studies; further applications of the comet assay. Mutat. Res. 2012, 736:122-129.