Visualization and stereological characterization of individual rat lung acini by high-resolution X-ray tomographic microscopy

Journal of Applied Physiology

Volumn 115, Issue 9, 2013, Pages 1379-1387

  Haberthür, David ^a,b   Barré, Sébastien F ^a   Tschanz, Stefan A ^a   Yao, Eveline ^a   Stampanoni, Marco ^b,c   Schittny, Johannes C ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b PAUL SCHERRER INSTITUTE   (Switzerland)

^c UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

(Number of) alveoli; Micro CT; Synchrotron radiation

Indexed keywords

(NUMBER OF) ALVEOLI; ACINAR CELL; ANIMAL; ARTICLE; COMPUTER ASSISTED TOMOGRAPHY; LUNG; LUNG GAS EXCHANGE; METHODOLOGY; MICRO-COMPUTED TOMOGRAPHY; MICROSCOPY; PHYSIOLOGY; RADIOGRAPHY; RAT; SPRAGUE DAWLEY RAT; SYNCHROTRON RADIATION; ULTRASTRUCTURE;

(NUMBER OF) ALVEOLI; MICRO-CT; SYNCHROTRON RADIATION;

ACINAR CELLS; ANIMALS; LUNG; MICROSCOPY; PULMONARY GAS EXCHANGE; RATS; RATS, SPRAGUE-DAWLEY; TOMOGRAPHY, X-RAY COMPUTED;

EID: 84887114846     PISSN: 87507587     EISSN: 15221601     Source Type: Journal    
DOI: 10.1152/japplphysiol.00642.2013     Document Type: Article

References (34)

1. Bitter I, Van Uitert R, Wolf I, Ibáñez L, Kuhnigk JM. Comparison of four freely available frameworks for image processing and visualization that use ITK. IEEE Trans Vis Comput Graph 13: 483-493, 2007.
2. Burri PH. The postnatal growth of the rat lung. 3. Morphology. Anat Rec 180: 77-98.
3. Cruz-Orive LM. Precision of Cavalieri sections and slices with local errors. J Microsc 193: 182-198, 1999.
4. Gehr P, Bachofen M, Weibel ER. The normal human lung: ultrastructure and morphometric estimation of diffusion capacity. Respir Physiol 32: 121-140, 1978.
5. Haberthür D, Hintermüller C, Marone F, Schittny JC, Stampanoni M. Radiation dose optimized lateral expansion of the field of view in synchrotron radiation X-ray tomographic microscopy. J Synchrotron Radiat 17: 590-599, 2010.
6. Haberthür D, Semmler-Behnke M, Takenaka S, Kreyling WG, Stampanoni M, Tsuda A, Schittny JC. Multimodal imaging for the detection of sub-micron particles in the gas-exchange region of the mammalian lung. J Phys Conf Ser 186: 012040, 2009.
7. Hsia CCW, Hyde DM, Ochs M, Weibel ER. An official research policy statement of the American Thoracic Society/European Respiratory Society: standards for quantitative assessment of lung structure. Am J Respir Crit Care Med 181: 394-418, 2010.
8. Hyde DM, Tyler NK, Putney LF, Singh P, Gundersen HG Jr. Total number and mean size of alveoli in mammalian lung estimated using fractionator sampling and unbiased estimates of the Euler characteristic of alveolar openings. Anat Rec A Discov Mol Cell Evol Biol 277: 216-226, 2004.
9. Knust J, Ochs M, Gundersen HJ, Nyengaard Jr. Stereological estimates of alveolar number and size and capillary length and surface area in mice lungs. Anat Rec (Hoboken) 292: 113-122, 2009.
10. Kumar H, Vasilescu DM, Yin Y, Hoffman EA, Tawhai MH, Lin CL. Multiscale imaging and registration-driven model for pulmonary acinar mechanics in the mouse. J Appl Physiol 114: 971-978, 2013.
11. Lorensen WE, Cline HE. Marching cubes: a high resolution 3D surface construction algorithm. ACM SIGGRAPH Computer Graphics 21: 163-169, 1987.
12. Mandelbrot B. How long is the coast of Britain? Statistical self-similarity and fractional dimension. Science 156: 636-638, 1967.
13. Mercer RR, Crapo JD. Three-dimensional reconstruction of the rat acinus. J Appl Physiol 63: 785-794, 1987.
14. Ochs M, Nyengaard JR, Jung A, Knudsen L, Voigt M, Wahlers T, Richter J, Gundersen HG Jr. The number of alveoli in the human lung. Am J Respir Crit Care Med 169: 120-124, 2004.
15. Rodriguez M, Bur S, Favre A, Weibel ER. Pulmonary acinus: geometry and morphometry of the peripheral airway system in rat and rabbit. Am J Anat 180: 143-155, 1987.
16. Schittny JC, Burri PH. Development and growth of the lung. In: Fishman's Pulmonary Diseases and Disorders (4th Ed.), edited by Fish- man A, Elias J, Fishman J, Grippi M, Senior R, Pack A. New York: McGraw-Hill Professional, 2008, chapt. 5, p. 91-114.
17. Schneiders R. A grid-based algorithm for the generation of hexahedral element meshes. Eng Comput 12: 168-177, 1996.
18. Sera T, Yokota H, Tanaka G, Uesugi K, Yagi N, Schroter RC. Murine pulmonary acinar mechanics during quasi-static inflation using synchrotron refraction-enhanced computed tomography. J Appl Physiol 115: 219-228, 2013.
19. Stampanoni M, Groso A, Isenegger A, Mikuljan G, Chen Q, Bertrand A, Henein S, Betemps R, Frommherz U, Böhler P, Meister D, Lange M, Abela R. Trends in synchrotron-based tomographic imaging: the SLS experience. In: Proceedings of SPIE, Society of Photo-Optical Instrumentation Engineers (SPIE) Conference Series, edited by Bonse U. Bellingham, WA: SPIE, 2006, vol. 6318, p. 63180M-63180M-14.
20. Sterio DC. The unbiased estimation of number and sizes of arbitrary particles using the disector. J Microsc 134: 127-136, 1984.
21. Thurlbeck WM. The internal surface area of nonemphysematous lungs. Am Rev Respir Dis 95: 765-773, 1967.
22. Tschanz SA, Burri PH, Weibel ER. A simple tool for stereological assessment of digital images: the STEPanizer. J Microsc 243: 47-59, 2011.
23. Tschanz SA, Makanya AN, Haenni B, Burri PH. Effects of neonatal high-dose short-term glucocorticoid treatment on the lung: a morphologic and morphometric study in the rat. Pediatr Res 53: 72-80, 2003.
24. Tsuda A, Filipovic N, Haberthür D, Dickie R, Matsui Y, Stampanoni M, Schittny JC. Finite element 3D reconstruction of the pulmonary acinus imaged by synchrotron X-ray tomography. J Appl Physiol 105: 964-976, 2008.
25. Vasilescu DM, Gao Z, Saha PK, Yin L, Wang G, Haefeli-Bleuer B, Ochs M, Weibel ER, Hoffman EA. Assessment of morphometry of pulmonary acini in mouse lungs by nondestructive imaging using multiscale microcomputed tomography. Proc Natl Acad Sci U S A 109: 17105-17110, 2012.
26. Weibel ER. Fractal geometry: a design principle for living organisms. Am J Physiol Lung Cell Mol Physiol 261: L361-L369, 1991.
27. Weibel ER. Morphometric and Stereological Methods in Respiratory Physiology Including Fixation Techniques. Amsterdam: Elsevier Scientific, 1984.
28. Weibel ER. Morphometric estimation of pulmonary diffusion capacity. Respir Physiol 11: 54-75, 1970.
29. Weibel ER. Morphometry of the Human Lung. Berlin: Springer-Verlag, 1963.
30. Woodward JD, Maina JN. A 3D digital reconstruction of the components of the gas exchange tissue of the lung of the muscovy duck, Cairina moschata. J Anat 206: 477-492, 2005.
31. Wulfsohn D, Knust J, Ochs M, Nyengaard JR, Gundersen HJ. Stereological estimation of the total number of ventilator units in mice lungs. J Microsc 238: 75-89, 2010.
32. Xiao L, Sera T, Koshiyama K, Wada S. A semiautomatic segmentation algorithm for extracting the complete structure of acini from synchrotron micro-CT images. Comput Math Methods Med 2013: 575086, 2013.
33. Zeltner TB, Bertacchini M, Messerli A, Burri PH. Morphometric estimation of regional differences in the rat lung. Exp Lung Res 16: 145-158, 1990.
34. Zucker SW. Region growing: childhood and adolescence. Comput Graph Image Proc 5: 382-399, 1976.