Constitutive expression of the xylanase inhibitor TAXI-III delays fusarium head blight symptoms in durum wheat transgenic plants

Molecular Plant-Microbe Interactions

Volumn 26, Issue 12, 2013, Pages 1464-1472

  Moscetti, Ilaria ^a   Tundo, Silvio ^a   Janni, Michela ^a   Sella, Luca ^b   Gazzetti, Katia ^b   Tauzin, Alexandra ^c   Giardina, Thierry ^c   Masci, Stefania ^a   Favaron, Francesco ^b   D'Ovidio, Renato ^a  

^a UNIVERSITY OF TUSCIA   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

^c AIX MARSEILLE UNIVERSITÉ   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ENDO 1,4 BETA XYLANASE; ENZYME INHIBITOR; FUNGAL PROTEIN; VEGETABLE PROTEIN;

ARTICLE; ASCOMYCETES; CEREAL; DISEASE RESISTANCE; DRUG ANTAGONISM; ENZYMOLOGY; FUSARIUM; GENE EXPRESSION REGULATION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PHYSIOLOGY; PLANT DISEASE; PLANT LEAF; SECRETION (PROCESS); TIME; TRANSGENIC PLANT; WHEAT;

ASCOMYCOTA; CEREALS; DISEASE RESISTANCE; ENDO-1,4-BETA XYLANASES; ENZYME INHIBITORS; FUNGAL PROTEINS; FUSARIUM; GENE EXPRESSION REGULATION, PLANT; PLANT DISEASES; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; TIME FACTORS; TRITICUM;

EID: 84887041466     PISSN: 08940282     EISSN: None     Source Type: Journal    
DOI: 10.1094/MPMI-04-13-0121-R     Document Type: Article

References (39)

1. Bailey, M. J., Biely, P., and Poutanen, K. 1992. Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 23:257-270.
2. Beliën, T., Van Campenhout, S., VanAcker, M., and Volckaert, G. 2005. Cloning and characterization of two endoxylanases from the cereal phytopathogen Fusarium graminearum and their inhibition profile against endoxylanase inhibitors from wheat. Biochem. Biophys. Res. Commun. 327:407-414. (Pubitemid 40040491)
3. Biely, P., Vrsanská, M., Tenkanen, M., and Kluepfel, D. 1997. Endo-beta1,4-xylanase families: Differences in catalytic properties. J Biotechnol. 57:151-66. (Pubitemid 27397479)
4. Brito, N., and Espino, J. J., and Gonzalez, C. 2006. The endo-beta-1,4-xylanase xyn11A is required for virulence in Botrytis cinerea. Mol Plant-Microbe Interact. 19:25-32. (Pubitemid 43011195)
5. Cantu, D., and Vicente, A. R., Labavitch, J. M., Bennett, A. B., and Powell, A. L. T. 2008. Strangers in the matrix: Plant cell walls and pathogen susceptibility. Trends Plant Sci. 13:610-617.
6. Christensen, A. H., and Quail, P. F. 1996. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res. 5:213-218. (Pubitemid 126603490)
7. Croes, E., Gebruers, K., Luyten, N., Delcour, J. A., and Courtin, C. M. 2009. The three classes of wheat xylanase-inhibiting proteins accumulate in an analogous way during wheat ear development and germination. J. Plant Physiol. 166:1253-1262.
8. Debyser, W., and Peumans, W. J., Van Damme E. J. M., and Delcour, J. A. 1999. Triticum aestivum xylanase inhibitor (TAXI), a new class of enzyme inhibitor affecting breadmaking performance. J. Cereal Sci. 30:39-43. (Pubitemid 129532268)
9. Dong, X., and Meinhardt, S. W., and Schwarz, P. B. 2012. Isolation and characterization of two endoxylanase from Fusarium graminearum. J. Agric. Food Chem. 60:2538-2545.
10. Dornez, E., Croes, E., Gebruers, K., Carpentier, S., Swennen, R., Laukens, K., Witters, E., Urban, M., Delcour, J. A., and Courtin, C. M. 2010a. 2D DIGE reveals changes in wheat xylanase inhibitor protein families due to Fusarium graminearum ΔTri5 infection and grain development. Proteomics 10:2303-2319.
11. Dornez, E., Croes, E., Gebruers, K., De Coninck, B., Cammue, B. P. A., Delcour, J. A., and Courtin, C. M. 2010b. Accumulated evidence substantiates a role for three classes of wheat xylanase inhibitors in plant defense. Crit. Rev. Plant Sci. 29:244-264.
12. Elliott, G., Durand, A., Hughes, R. K., D'Ovidio, R., and Juge, N. 2009. Isolation and molecular characterisation of a novel xylanase inhibitor gene from durum wheat. J. Cereal Sci. 50:324-331.
13. Elliott, G. O., and Hughes, R. K., Juge, N., Kroon, P. A., and Williamson, G. 2002. Functional identification of the cDNA coding for a wheat endo1,4-beta-D-xylanase inhibitor. FEBS (Fed. Eur. Biochem. Soc.) Lett. 519:66-70. (Pubitemid 34521993)
14. Emami, K., and Hack, E. 2001. Characterization of xylanase gene from Cochliobolus sativus and its expression. Mycol. Res. 105:352-359. (Pubitemid 32258469)
15. Ferrari, S., Sella, L., Janni, M., De Lorenzo, G., Favaron, F., and D'Ovidio, R. 2012. Transgenic expression of polygalacturonase-inhibiting proteins in Arabidopsis and wheat increases resistance to the flower pathogen Fusarium graminearum. Plant Biol. 14:31-38.
16. Fierens, E., Rombouts, S., Gebruers, K., Goesaert, H., Brijs, K., Beaugrand, J., Volckaert, G., Van Campenhout, S., Proost, P., Courtin, C. M., and Delcour, J. A. 2007. TLXI, a novel type of xylanase inhibitor from wheat (Triticum aestivum) belonging to the thaumatin family. Biochem. J. 403:583-591. (Pubitemid 46706987)
17. Gebruers, K., Brijs, K., Courtin, C. M., Fierens, K., Goesaert, H., Rabijns, A., Raedschelders, G., Robben, J., Sansen, S., Sorensen, J. F., Van Campenhout, S., and Delcour, J. A. 2004. Properties of TAXI-type endoxylanase inhibitors. Biochim. Biophys. Acta 1696:213-221. (Pubitemid 38202261)
18. Horevaj, P., and Milus, E. A., and Bluhm, B. H. 2011. A real-time qPCR assay to quantify Fusarium graminearum biomass in wheat kernels. J. Appl. Microbiol. 111:396-406.
19. Igawa, T., Ochiai-Fukuda, T., Takahashi-Ando, N., Ohsato, S., Takehico, S., Yamaguki, I., and Kimura, M. 2004. New TAXI-type xylanase inhibitor genes are inducible by pathogens and wounding in hexaploid wheat. Plant Cell Physiol. 45:1347-1360. (Pubitemid 39547619)
20. Igawa, T., Tokai, T., Kudo, T., Yamaguchi, I., and Kimura, M. 2005. A wheat xylanase inhibitor gene, Xip-I, but not Taxi - I, is significantly induced by biotic and abiotic signals that trigger plant defense. Biosci. Biotechnol. Biochem. 69:1058-1063. (Pubitemid 41006988)
21. Janni, M., Sella, L., Favaron, F., Blechl, A. E., De Lorenzo, G., and D'Ovidio, R. 2008. The expression of a bean PGIP in transgenic wheat confers increased resistance to the fungal pathogen Bipolaris sorokiniana. Mol. Plant-Microbe Interact. 21:171-177.
22. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
23. Livak, K. J., and Schmittgen, T. D. 2001. Analysis of relative gene expression data using Real-Time 17 quantitative PCR and 2-ΔΔCT method. Methods 25:402-408. (Pubitemid 34164012)
24. Lu, S., and Friesen, T. L., and Faris, J. D. 2011. Molecular characterization and genomic mapping of the pathogenesis-related protein 1 (PR-1) gene family in hexaploid wheat (Triticum aestivum L.) Mol. Genet. Genomics 285:485-503.
25. Lu, Z. X., Gaudet, D., Puchalski, B., Despins, T., Frick, M., and Laroche, A. 2006. Inducers of resistance reduce common bunt infection in wheat seedlings while differentially regulating defence-gene expression. Physiol. Mol. Plant P. 67:138-148. (Pubitemid 43795427)
26. Lysøe, E., Seong, K., and Kistler, H. C. 2011. The transcriptome of Fusarium graminearum during infection of wheat. Mol. Plant-Microbe Interact. 24:995-1000.
27. McLauchlan, W. R., Garcia-Conesa, M. T., Williamson, G., Roza, M., Ravestein, P., and Maat, J. 1999. A novel class of protein from wheat which inhibits xylanases. Biochem. J. 338:441-446.
28. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31:426-428.
29. Neuhoff, V., Arold, N., Taube, D., and Ehrhardt, W. 1998. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9:255-262.
30. Paper, J. M., Scott-Craig, J. S., Adhikari, N. D., Cuomo, C. A., and Walton, J. D. 2007. Comparative proteomics of extracellular proteins in vitro and in planta from the pathogenic fungus Fusarium graminearum. Proteomics 7:3171-3183. (Pubitemid 47477959)
31. Pollet, A., Beliën, T., Fierens, K., and Delcour, J. A., and Courtin, C. M. 2009. Fusarium graminearum xylanases show different functional stabilities, substrate specificities and inhibition sensitivities. Enzyme Microb. Technol. 44:189-195.
32. Raedschelders, G., Fierens, K., Sansen, S., Rombouts, S., Gebruers, K., Robben, J., Rabijns, A., Courtin, C. M., Delcour, J. A., Van Campenhout, S., and Volckaert, G. 2005. Molecular identification of wheat endoxylanase inhibitor TAXI-II and the determinants of its inhibition specificity. Biochem. Biophys. Res. Commun. 335:512-522. (Pubitemid 41140252)
33. Royer, J. C., and Nakas, J. P. 1990. Interrelationship of xylanase induction and cellulase induction of Trichoderma longibrachiatum. Appl. Environ. Microbiol. 56:2535-2539. (Pubitemid 20235701)
34. Salvi, G., Giarrizzo, F., De Lorenzo, G., and Cervone, F. 1990. A Polygalacturnase-Inhibiting Protein in the flowers of Phaseolus vulgaris. L. J. Plant Physiol. 136:513-518.
35. Sella, L., Gazzetti, K., Faoro, F., Odorizzi, S., D'Ovidio, R., Schäfer, W., and Favaron, F. 2013. A Fusarium graminearum xylanase expressed during wheat infection is a necrotizing factor but is not essential for virulence. Plant Physiol. Biochem. 64:1-10.
36. Tai, T. H., and Tanksley, S. D. 1991. A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue. Plant Mol. Biol. Rep. 8:297-303.
37. Takahashi-Ando, N., Inaba, M., Ohsato, S., Igawa, T., Usami, R., and Kimura, M. 2007. Identification of multiple highly similar XIP-type xylanase inhibitor genes in hexaploid wheat. Biochem. Biophys. Res. Commun. 360:880-884. (Pubitemid 47082438)
38. Urban, U., Daniels, S., Mott, E., and Hammond-Kosack, K. 2002. Arabidopsis is susceptible to the cereal ear blight fungal pathogens Fusarium graminearum and Fusarium culmorum. Plant J. 32:961-973. (Pubitemid 36108063)
39. Volpi, C., Janni, M., Lionetti, V., Bellincampi, D., Favaron, F., and D'Ovidio, R. 2011. The ectopic expression of a pectin methyl esterase inhibitor increases pectin methyl esterification and limits fungal diseases in wheat. Mol. Plant-Microbe Interact. 24:1012-1019.