Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran

Iranian Journal of Microbiology

Volumn 5, Issue 3, 2013, Pages 209-214

  Nikbin, Vajiheh Sadat ^a   Shahcheraghi, Fereshteh ^a   Lotfi, Masoumeh Nakhost ^a   Zahraei, Seyyed Mohsen ^b   Parzadeh, Masoumeh ^a  

^a PASTEUR INSTITUTE OF IRAN   (Iran)

^b MINISTRY OF HEALTH AND MEDICAL EDUCATION   (Iran)

Author keywords

Bordetella pertussis; BP283; IS481; Real time PCR

Indexed keywords

AGGLUTINATION TEST; ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM DETECTION; BORDETELLA PERTUSSIS; BP283 GENE; CHILD; CONTROLLED STUDY; FEMALE; GENE INSERTION SEQUENCE; HUMAN; INTERMETHOD COMPARISON; IS481 GENE; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PERTUSSIS; PRESCHOOL CHILD; REAL TIME POLYMERASE CHAIN REACTION; SCHOOL CHILD; SENSITIVITY ANALYSIS;

EID: 84887032325     PISSN: 20083289     EISSN: 20084447     Source Type: Journal    
DOI: None     Document Type: Article

References (44)

1. Tozzi AE, Celentan LP, Ciofi degli Atti ML, Salmaso S. Diagnosis and management of pertussis. CMAJ 2005; 172: 509-515.
2. Wood N, McIntyre P. Pertussis: review of epidemiology, diagnosis, management and prevention. Paediatr Respir Rev 2008; 9: 201-212.
3. World Health Organization (WHO) 2007. Laboratory manual for the diagnosis of whooping cough caused by Bordetella pertussis/Bordetella parapertussis.
4. Bidet P, Liguori S, deLauzanne A, Caro V, Lorrot M, Carol A et al. Real-time PCR measurement of persistence of Bordetella pertussis DNA in nasopharyngeal secretions during antibiotic treatment of young children with pertussis. J Clin Microbiol 2008; 46: 3636-3638.
5. de Melker HE, Conyn-van Spaendonck MA, Rumke HC, van Wijngaarden JK, Mooi FR, et al. Pertussis in The Netherlands: anoutbreak despite high levels of immunization with whole-cell vaccine. Emerg Infect Dis 1997; 3: 175-178.
6. Elomaa A, Advani A, Donnelly D, Antila M, Mertsola J, He Q, et al. Population dynamics of Bordetella pertussis in Finland and Sweden, neighbouring countries with different vaccination histories. Vaccine 2007; 25: 918-26.
7. Elomaa A, Advani, A, Donnelly D, Antila M, Mertsola J, Hallander H, et al. Strain variation among Bordetella pertussis isolates in Finland where the whole-cell pertussis vaccine has been used for 50 years. J Clin Microbiol 2005; 43: 3681-3687.
8. Poynten M, McIntyre P., Mooi FR, Heuvelman KJ, Gilbert GL. Temporal trends in circulating Bordetella pertussis strains in Australia. Epidemiol Infect 2004; 132: 185-193.
9. Weber C, Boursaux-Eude C, Coralie G, Caro V, Guiso N. Polymorphism of Bordetella pertussis isolates circulating for the last 10 years in France, where a single effective whole-cell vaccine has been used for more than 30 years. J Clin Microbiol 2001; 39: 4396-403.
10. Bass JW, Stephenson SR. The return of pertussis. Pediatr Infect Dis J 1987; 6: 141-144.
11. He Q, Mäkinen J, Berbers G, Mooi FR, Viljanen MK, Arvilommi H, et al. Bordetella pertussis protein pertactin induces type-specific antibodies: one possible explanation for the emergence of antigenic variants? J Infect Dis 2003; 187: 1200-1205.
12. Mooi FR, van Oirschot H, Heuvelman K, van der Heide HG, Gaastra W, Willems RJ. Polymorphism in the Bordetella pertussis virulence factors P.69/pertactin and pertussis toxin in The Netherlands: temporal trends and evidence for vaccine-driven evolution. Infect Immun 1998; 66: 670-675.
13. Ochman H, A. Wilson C. Evolution in bacteria: evidence for a universal substitution rate in cellular genomes. J Mol Evol 1987; 6: 74-86.
14. Willems RJ., Mooi FR. From whole cell to acellular vaccines. Rev Med Microbiol 1996; 7: 13-21.
15. Dragsted DM, Dohn B, Madsen J, Jensen JS. Comparison of culture and PCR for detection of Bordetella pertussis and Bordetella parapertussis under routine laboratory conditions. J Med Microbiol 2004; 53: 749-754.
16. Kösters K, Riffelmann M, von König CHW. Evaluation of a real-time PCR assay for detection of Bordetella pertussis and Bordetella. parapertussis in clinical samples. J Med Microbiol 2001; 50: 436-440.
17. Kösters K, Reischl U, Schmetz J, Riffelmann M, von König CHW. Real-Time Light Cycler PCR for Detection and Discrimination of Bordetella pertussis and Bordetella parapertussis. J Clin Microbiol 2002; 40: 1719-1722.
18. Loe elholz M J, Thompson CJ, Long KS, Gil-christ MJ. Comparison of PCR, culture, and direct fluorescentantibody testing for detection of Bordetella pertussis. J Clin Microbiol 1999; 37: 2872-2876.
19. Antila M, He Q, de Jong C, Aarts I, Verbakel H, Bruisten S et al. Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis. J Med Microbiol 2006; 55: 1043-1051.
20. Koidi C, Bozic M, Burmeister A, Hess M, Marth E, Kessler HH. Detection and Differentiation of Bordetella spp. by Real-Time PCR. J Clin Microbiol 2007; 45: 347-350.
21. Xu Y, Xu Y, Hou Q, Yang R, Zhang S. Triplex realtime PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis. A P M I S 2010; 118: 685-691.
22. Parkhill J, Sebaihia M, Preston A, Murphy LD, Thomson N, Harris DE, et al. Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nat Genet 2003; 35: 32-40.
23. Register KB, Sanden GN. Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implication for IS481-based detection of Bordetella pertussis. J Clin Microbiol 2006; 44: 4577-4583.
24. Reischl U, Lehn N, Sanden GN, Loeffelholz MJ. Realtime PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii. J Clin Microbiol 2001; 39: 1963-1966.
25. Vincart B, deMendonça R, Rottiers S, Vermeulen F, Struelens MJ, Denis O. A specific real-time PCR assay for the detection of Bordetella pertussis. J Med Microbiol 2007; 56: 918-920.
26. Fry NK, Tzivra O, Li YT, McNiff A, Doshi N, Maple PA, et al. Laboratory diagnosis of pertussis infections: the role of PCR and serology. J Med Microbiol 2004; 53: 519-525.
27. Muyldermans G, Soetens O, Antoine M, Bruisten S, Vincart B, Doucet K, et al. External quality assessment for molecular detection of Bordetella pertussis in European laboratories. J Clin Microbiol 2005; 43: 30-35.
28. Furuya D, Yagihashi A, Endoh T, Uehara N, Fujii N, Chiba S, et al. Simultaneous amplification of Bordetella repeated insertion sequences and toxin promoter region gene by polymerase chain reaction. Immunopharmocol Immunotoxicol 1999; 21: 55-63.
29. Matthews RC, Golbang N, Bruck WM, Owen D, Bailey A, Weston V, et al. Semiquantitative polymerase chain reaction enzyme immunoassay for the diagnosis of pertussis. Eur J Clin Microbiol Infect Dis 1999; 18: 748-750.
30. Guthrie JL, Seah C, Brown S, Tang P, Jamieson F, Drews SJ. Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay. J Clin Microbiol 2008; 46: 3798-3799.
31. Probert WS, Ely J, Schrader K, Atwell J, Nossoff A, Kwan S. Identification and Evaluation of New Target Sequences for Specific Detection of Bordetella pertussis by Real-Time PCR. J Clin Microbiol 2008; 46: 3228-3231.
32. McGowan KL (2004). Bordetella cultures. In: H. D. Isenberg (ed.), Clinical microbiology procedures handbook, 2nd ed., vol. 1. American Society for Microbiology, Washington, D.C, p. 3.3.2.1-3.3.2.14.
33. th edition, Philadelphia: Lippincott Williams & Wilkins.
34. van der Zee A, Mooi F, Van Embden J, Musser J. Molecular evolution and host adaptation of Bordetella spp.: phylogenetic analysis using multilocus enzyme electrophoresis and typing with three insertion sequences. J Bacteriol 1997; 179: 6609-6617.
35. Mazengia E, Silva EA, Peppe JA, Timperi R, George H. Recovery of Bordetella holmesii from patients with pertussis-like symptoms: use of pulsed-field gel electrophoresis to characterize circulating strains. J Clin Microbiol 2000; 38: 2330-2333.
36. Yih WK, Silva EA, Ida J, Harrington N, Lett SM, George H. Bordetella holmesii-like organisms isolated from Massachusetts patients with pertussis-like symptoms. Emerg Infect Dis 1999; 5: 441-443.
37. Knorr L, Fox JD, Tilley PA, Ahmed-Bentley J. Evaluation of real-time PCR for diagnosis of Bordetella pertussis infection. BMC Infect Dis 2006; 6: 62-67.
38. Qin, X, Galanakis E, Martin ET, Englund JA. Multitarget PCR for diagnosis of pertussis and itsclinical implications. J Clin Microbiol 2007; 45: 506-511.
39. Farrell DJ, McKeon M, Daggard G, Loeffelholz MJ, Thompson CJ, Mukkur TKS. Rapid-cycle PCR method to detect Bordetella pertussis that fulfills all consensus recommendations for use of PCR in diagnosis of pertussis. J Clin Microbiol 2000; 38: 4499-4502.
40. Fry NK, Duncan J, Wagner K, Tzivra O, Doshi N, Litt DJ, et al. Role of PCR in the diagnosis of pertussis infection in infants: 5 years' experience of provision of a same-day real-time PCR service in England and Wales from 2002 to 2007. J Med Microbiol 2009; 58: 1023-1029.
41. Cimolai, N, Trombley C. O'Neill D. Diagnosis of whooping cough: a new era with rapid molecular diagnostics. Pediatr Emerg Care 1996; 12: 91-93.
42. Nakamura Y, Kamachi K, Toyoizumi-Ajisaka H, Otsuka N, Saito R, Tsuruoka J, et al. Marked difference between adults and children in Bordetella pertussis DNA load in nasopharyngeal swabs. Clin Microbiol Infect 2011; 17: 365-370.
43. Crowcroft N, Stein C, Duclos P, Birmingham M. How best to estimate the global burden of pertussis? Lancet Infect Dis 2003; 3: 413-418.
44. Forsyth K, Campins-Marti M, Caro J, Cherry JD, Greenberg D, Guiso N, et al. New pertussis vaccination strategies beyond infancy: recommendations by the global pertussis initiative. Clin Infect Dis 2004; 39: 1802-1809.