Elucidating heterogeneity of IgA1 hinge-region O-glycosylation by use of MALDI-TOF/TOF mass spectrometry: Role of cysteine alkylation during sample processing

Journal of Proteomics

Volumn 92, Issue , 2013, Pages 299-312

  Franc, Vojtěch ^a   Řehulka, Pavel ^b   Raus, Martin ^a   Stulík, Jiří ^b   Novak, Jan ^c   Renfrow, Matthew B ^d   Šebela, Marek ^a  

^a PALACKÝ UNIVERSITY   (Czech Republic)

^b UNIVERSITY OF DEFENCE   (Czech Republic)

^c University of Alabama at Birmingham   (United States)

^d UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

Glycopeptide; Human immunoglobulin A1 (IgA1); IgA nephropathy; Mass spectrometry; Microgradient separation; O glycosylation

Indexed keywords

CYSTEINE; GALACTOSE; GLYCAN; IMMUNOGLOBULIN A1; N ACETYLGALACTOSAMINE; N ACETYLGLUCOSAMINE; PARAPROTEIN;

ALKYLATION; ARTICLE; BINDING SITE; GLYCOSYLATION; HINGE REGION; HUMAN; ISOMER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN POLYMORPHISM; REVERSED PHASE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY; TIME OF FLIGHT MASS SPECTROMETRY;

ACETONITRILE; ACN; CAM; CARBAMIDOMETHYLATION; CHCA; DITHIOTHREITOL; DTT; ECD; ELECTRON CAPTURE DISSOCIATION; ELECTRON TRANSFER DISSOCIATION; ETD; FEP; FLUORINATED ETHYLENE PROPYLENE; FOURIER TRANSFORM ION CYCLOTRON RESONANCE; FT-ICR; GLYCOPEPTIDE; HINGE REGION; HR; HUMAN IMMUNOGLOBULIN A1 (IGA1); IAM; IGA; IGA NEPHROPATHY; IGAN; IMMUNOGLOBULIN A; IODOACETAMIDE; LC; LIQUID CHROMATOGRAPHY; MALDI-TOF; MASS SPECTROMETRY; MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT; MICROGRADIENT SEPARATION; MS; MS/MS; O-GLYCOSYLATION; PAM; POST-SOURCE DECAY; PROPIONAMIDATION; PSD; REVERSED-PHASE LIQUID CHROMATOGRAPHY; RPLC; TANDEM MASS SPECTROMETRY; TFA; TRIFLUOROACETIC ACID; Α-CYANO-4-HYDROXYCINNAMIC ACID;

ALKYLATION; CYSTEINE; GLOMERULONEPHRITIS, IGA; GLYCOSYLATION; HUMANS; IMMUNOGLOBULIN A; SPECIMEN HANDLING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84886950647     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2013.07.013     Document Type: Article

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