Optimized method for identification of the proteomes secreted by cardiac cells

Methods in Molecular Biology

Volumn 1005, Issue , 2013, Pages 225-235

  Stastna, Miroslava ^a,b   Van Eyk, Jennifer E ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b INSTITUTE OF ANALYTICAL CHEMISTRY   (Czech Republic)

Author keywords

Cardiac cells; Conditioned medium; Proteomics; Reversed phase liquid chromatography; Secreted proteomes; Tandem mass spectrometry

Indexed keywords

PROTEOME; TRYPSIN; PEPTIDE FRAGMENT;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL COUNTING; CELL CULTURE; CELL VIABILITY; CONTROLLED STUDY; HEART MUSCLE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN SECRETION; RAT; REVERSED PHASE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY; ANIMAL; CHEMISTRY; CULTURE MEDIUM; CYTOLOGY; METABOLISM; METHODOLOGY; NEWBORN; PEPTIDE MAPPING; PRIMARY CELL CULTURE; PROTEIN DEGRADATION; SECRETION (PROCESS);

ANIMALS; ANIMALS, NEWBORN; CHROMATOGRAPHY, REVERSE-PHASE; CULTURE MEDIA, CONDITIONED; MYOCYTES, CARDIAC; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PRIMARY CELL CULTURE; PROTEOLYSIS; PROTEOME; RATS; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84886920989     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-386-2_18     Document Type: Article

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