Proteomic and metabolomic responses to connexin43 silencing in primary hepatocyte cultures

Archives of Toxicology

Volumn 87, Issue 5, 2013, Pages 883-894

  Vinken, Mathieu ^a   Maes, Michaël ^a   Cavill, Rachel ^b,c   Valkenborg, Dirk ^d,e,f   Ellis, James K ^b   Decrock, Elke ^g   Leybaert, Luc ^g   Staes, An ^h,i   Gevaert, Kris ^h,i   Oliveira, André G ^j   Menezes, Gustavo B ^j   Cogliati, Bruno ^k   Dagli, Maria Lúcia Zaidan ^k   Ebbels, Timothy M D ^b   Witters, Erwin ^d,e   Keun, Hector C ^b   Vanhaecke, Tamara ^a   Rogiers, Vera ^a  

^a FACULTY OF MEDICINE AND PHARMACY   (Belgium)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c MAASTRICHT UNIVERSITY   (Netherlands)

^d FLEMISH INSTITUTE FOR TECHNOLOGICAL RESEARCH VITO   (Belgium)

^e UNIVERSITY OF ANTWERP   (Belgium)

^f HASSELT UNIVERSITY   (Belgium)

^g GHENT UNIVERSITY   (Belgium)

^h DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

ⁱ GHENT UNIVERSITY   (Belgium)

^j FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^k UNIVERSITY OF SÃO PAULO   (Brazil)

more..

Author keywords

Connexin43; Metabolomics; Primary hepatocyte; Proteomics

Indexed keywords

CHAPERONIN 60; CONNEXIN 32; CONNEXIN 43; CYTOCHROME P450 2C; CYTOCHROME P450 2C70; GLUCOSE REGULATED PROTEIN; GLUCOSE REGULATED PROTEIN 75; GLUTATHIONE TRANSFERASE; GLYCINE; PROTEOME; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; THIOSULFATE SULFURTRANSFERASE; UNCLASSIFIED DRUG; BIOLOGICAL MARKER; SMALL INTERFERING RNA;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL MEMBRANE; CYTOPLASM; GENE EXPRESSION; GENE SILENCING; IMMUNOBLOTTING; LIVER CELL CULTURE; METABOLOME; METABOLOMICS; MITOCHONDRION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIMARY CELL CULTURE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; PROTON NUCLEAR MAGNETIC RESONANCE; RAT; RNA INTERFERENCE; TREATMENT OUTCOME; WESTERN BLOTTING; ANIMAL; CELL CULTURE; GENETICS; LIVER CELL; LIVER MITOCHONDRION; MALE; METABOLISM; NUCLEAR MAGNETIC RESONANCE; OUTBRED STRAIN; SIGNAL TRANSDUCTION; SPRAGUE DAWLEY RAT; TANDEM MASS SPECTROMETRY;

ANIMALS; ANIMALS, OUTBRED STRAINS; BIOLOGICAL MARKERS; CELLS, CULTURED; CONNEXIN 43; GENE SILENCING; HEPATOCYTES; MALE; METABOLOMICS; MITOCHONDRIA, LIVER; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEOMICS; RATS; RATS, SPRAGUE-DAWLEY; RNA INTERFERENCE; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; TANDEM MASS SPECTROMETRY;

EID: 84886914881     PISSN: 03405761     EISSN: 14320738     Source Type: Journal    
DOI: 10.1007/s00204-012-0994-0     Document Type: Article

References (30)

1. Boengler K, Ruiz-Meana M, Gent S, Ungefug E, Soetkamp D, Miro-Casas E, Cabestrero A, Fernandez-Sanz C, Semenzato M, Di Lisa F, Rohrbach S, Garcia-Dorado D, Heusch G, Schulz R (2012) Mitochondrial connexin 43 impacts on respiratory complex I activity and mitochondrial oxygen consumption. J Cell Mol Med 16:1649-1655
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
3. Decrock E, Vinken M, De Vuyst E, Krysko DV, D'Herde K, Vanhaecke T, Vandenabeele P, Rogiers V, Leybaert L (2009) Connexin-related signaling in cell death: to live or let die Cell Death Differ 16:524-536
4. Decrock E, Vinken M, Bol M, D'Herde K, Rogiers V, Vandenabeele P, Krysko DV, Bultynck G, Leybaert L (2011) Calcium and connexin-based intercellular communication, a deadly catch? Cell Calcium 50:310-321
5. Dieterle F, Ross A, Schlotterbeck G, Senn H (2006) Probabilistic quotient normalization as robust method to account for dilution of complex biological mixtures: application in 1H NMR metabonomics. Anal Chem 78:4281-4290
6. Elaut G, Henkens T, Papeleu P, Snykers S, Vinken M, Vanhaecke T, Rogiers V (2006) Molecular mechanisms underlying the dedifferentiation process of isolated hepatocytes and their cultures. Curr Drug Metab 7:629-660
7. Ellis JK, Athersuch TJ, Cavill R, Radford R, Slattery C, Jennings P, McMorrow T, Ryan MP, Ebbels TM, Keun HC (2011) Metabolic response to low-level toxicant exposure in a novel renal tubule epithelial cell system. Mol BioSyst 7:247-257
8. Goubaeva F, Mikami M, Giardina S, Ding B, Abe J, Yang J (2007) Cardiac mitochondrial connexin43 regulates apoptosis. Biochem Biophys Res Commun 352:97-103
9. Hewitt NJ, Lechon MJ, Houston JB, Hallifax D, Brown HS, Maurel P, Kenna JG, Gustavsson L, Lohmann C, Skonberg C, Guillouzo A, Tuschl G, Li AP, LeCluyse E, Groothuis GM, Hengstler JG (2007) Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies. Drug Metab Rev 39:159-234
10. Kersey PJ, Duarte J, Williams A, Karavidopoulou Y, Birney E, Apweiler R (2004) The International Protein Index: an integrated database for proteomics experiments. Proteomics 4:1985-1988
11. Klee P, Allagnat F, Pontes H, Cederroth M, Charollais A, Caille D, Britan A, Haefliger JA, Meda P (2011) Connexins protect mouse pancreatic b cells against apoptosis. J Clin Invest 121:4870-4879
12. Kocher T, Pichler P, Schutzbier M, Stingl C, Kaul A, Teucher N, Hasenfuss G, Penninger JM, Mechtler K (2009) High precision quantitative proteomics using iTRAQ on an LTQ Orbitrap: a new mass spectrometric method combining the benefits of all. J Proteome Res 8:4743-4752
13. Kon K, Kim JS, Jaeschke H, Lemasters JJ (2004) Mitochondrial permeability transition in acetaminophen-induced necrosis and apoptosis of cultured mouse hepatocytes. Hepatology 40:1170-1179
14. Luntz SP, Unnebrink K, Seibert-Grafe M, Bunzendahl H, Kraus TW, Buchler MW, Klar E, Schemmer P (2005) HEGPOL: randomized, placebo controlled, multicenter, double-blind clinical trial to investigate hepatoprotective effects of glycine in the postoperative phase of liver transplantation [ISRCTN69350312]. BMC Surg 5:18
15. Menger MD, Vollmar B (2007) Pathomechanisms of ischemiareperfusion injury as the basis for novel preventive strategies: is it time for the introduction of pleiotropic compoundš Transplant Proc 39:485-488
16. Miro-Casas E, Ruiz-Meana M, Agullo E, Stahlhofen S, Rodríguez- Sinovas A, Cabestrero A, Jorge I, Torre I, Vazquez J, Boengler K, Schulz R, Heusch G, Garcia-Dorado D (2009) Connexin43 in cardiomyocyte mitochondria contributes to mitochondrial potassium uptake. Cardiovasc Res 83:747-756
17. Olk S, Turchinovich A, Grzendowski M, Stuhler K, Meyer HE, Zoidl G, Dermietzel R (2010) Proteomic analysis of astroglial connexin43 silencing uncovers a cytoskeletal platform involved in process formation and migration. Glia 58:494-505
18. Papeleu P, Vanhaecke T, Henkens T, Elaut G, Vinken M, Snykers S, Rogiers V (2006) Isolation of rat hepatocytes. Methods Mol Biol 320:229-237
19. Raza H (2011) Dual localization of glutathione S-Transferase in the cytosol and mitochondria: implications in oxidative stress, toxicity and disease. FEBS J 278:4243-4251
20. Rottlaender D, Boengler K, Wolny M, Schwaiger A, Motloch LJ, Ovize M, Schulz R, Heusch G, Hoppe UC (2012) Glycogen synthase kinase 3b transfers cytoprotective signaling through connexin 43 onto mitochondrial ATP-sensitive K? channels. Proc Natl Acad Sci USA 109:E242-E251
21. Vandenbroucke RE, De Geest BG, Bonne S, Vinken M, Vanhaecke T, Heimberg H, Wagner E, Rogiers V, De Smedt SC, Demeester J, Sanders NN (2008) Prolonged gene silencing in hepatoma cells and primary hepatocytes after small interfering RNA delivery with biodegradable poly(beta-Amino esters). J Gene Med 10:783-794
22. Vinken M, Vanhaecke T, Papeleu P, Snykers S, Henkens T, Rogiers V (2006a) Connexins and their channels in cell growth and cell death. Cell Signal 18:592-600
23. Vinken M, Papeleu P, Snykers S, De Rop E, Henkens T, Chipman JK, Rogiers V, Vanhaecke T (2006b) Involvement of cell junctions in hepatocyte culture functionality. Crit Rev Toxicol 36:299-318
24. Vinken M, Henkens T, De Rop E, Fraczek J, Vanhaecke T, Rogiers V (2008) Biology and pathobiology of gap junctional channels in hepatocytes. Hepatology 47:1077-1088
25. Vinken M, Decrock E, De Vuyst E, Ponsaerts R, D'hondt C, Bultynck G, Ceelen L, Vanhaecke T, Leybaert L, Rogiers V (2011a) Connexins: sensors and regulators of cell cycling. Biochim Biophys Acta 1815:13-25
26. Vinken M, Decrock E, Doktorova T, Ramboer E, De Vuyst E, Vanhaecke T, Leybaert L, Rogiers V (2011b) Characterization of spontaneous cell death in monolayer cultures of primary hepatocytes. Arch Toxicol 85:1589-1596
27. Vinken M, Buyl K, Vanhaecke T, Rogiers V (2012a) Effects of RNA interference-mediated suppression of connexin43 production on the expression of differentiation markers in cultures of adult primary rat hepatocytes. ALTEX Proc 1:155-164
28. Vinken M, Decrock E, Vanhaecke T, Leybaert L, Rogiers V (2012b) Connexin43 signaling contributes to spontaneous apoptosis in cultures of primary hepatocytes. Toxicol Sci 125:175-186
29. Yamaji S, Droggiti A, Lu SC, Martinez-Chantar ML, Warner A, Varela-Rey M (2011) S-Adenosylmethionine regulates connexins subtypes expressed by hepatocytes. Eur J Cell Biol 90:312-322
30. Yang YH, Dudoit S, Luu P, Lin DM, Peng V, Ngai J, Speed TP (2002) Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Res 30:e15