Characterization of zebra fish cardiac proteome using online ph gradient SCX-RP HPLC-MS/MS platform

Methods in Molecular Biology

Volumn 1005, Issue , 2013, Pages 119-127

  Zhang, Jiang ^a   Lanham, Kevin A ^b   Heideman, Warren ^b   Peterson, Richard E ^a   Li, Lingjun ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

2D HPLC; Cardiac; Mass spectrometry; PH gradient; Proteome; SCX RP; Zebrafish

Indexed keywords

PROTEOME; TRYPSIN;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICROWAVE RADIATION; NONHUMAN; PH; PH GRADIENT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEOMICS; SCX RP METHOD; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ZEBRA FISH; ANIMAL; CHEMISTRY; GENE EXPRESSION; GENETICS; HEART MUSCLE; ION EXCHANGE CHROMATOGRAPHY; METABOLISM; METHODOLOGY; PEPTIDE MAPPING; PROTEIN DEGRADATION; PROTON MOTIVE FORCE; REVERSED PHASE LIQUID CHROMATOGRAPHY;

ANIMALS; CHROMATOGRAPHY, ION EXCHANGE; CHROMATOGRAPHY, REVERSE-PHASE; GENE EXPRESSION; MICROWAVES; MYOCARDIUM; PEPTIDE MAPPING; PROTEOLYSIS; PROTEOME; PROTON-MOTIVE FORCE; TANDEM MASS SPECTROMETRY; TRYPSIN; ZEBRAFISH;

EID: 84886889794     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-386-2_10     Document Type: Article

References (16)

1. Bakkers J (2011) Zebra fish as a model to study cardiac development and human cardiac disease. Cardiovasc Res 91:279-288
2. Carney SA et al (2006) Understanding dioxin developmental toxicity using the zebra fish model. Wiley. Hoboken, New Jersey, USA p 7-18
3. Heideman W et al (2005) Zebra fish and cardiac toxicology. Cardiovasc Toxicol 5:203-214
4. Washburn MP, Wolters D, Yates JR (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol 19:242-247
5. Zhang J et al (2010) Characterization of the adult zebra fish cardiac proteome using online pH gradient strong cation exchange-RP 2D LC coupled with ESI MS/MS. J Sep Sci 33:1462-1471
6. Dowell JA et al (2008) Comparison of twodimensional fractionation techniques for shotgun proteomics. Anal Chem 80:6715-6723
7. Garcia MC (2005) The effect of the mobile phase additives on sensitivity in the analysis of peptides and proteins by high-performance liquid chromatography-electrospray mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 825:111-123
8. Sluyterman LAA, Wijdenes J (1978) Chromatofocusing-isoelectric-focusing on ionexchange columns. 2. Experimental verification. J Chromatogr 150:31-44
9. Sluyterman LAA, Wijdenes J (1981) Chromatofocusing. 4. Properties of an agarose polyethyleneimine ion-exchanger and its suitability for protein separations. J Chromatogr 206:441-447
10. Sluyterman LAA, Wijdenes J (1981) Chromatofocusing. 3. The properties of a DEAEagarose anion-exchanger and its suitability for protein separations. J Chromatogr 206:429-440
11. Kang XZ, Frey DD (2003) High-performance cation-exchange chromatofocusing of proteins. J Chromatogr A 991:117-128
12. Kang XZ, Frey DD (2004) Chromatofocusing of peptides and proteins using linear pH gradients formed on strong ion-exchange adsorbents. Biotechnol Bioeng 87:376-387
13. Pepaj M et al (2006) Fractionation and separation of human salivary proteins by pH -gradiention exchange and reversed phase chromatography coupled to mass spectrometry. J Sep Sci 29:519-528
14. Pepaj M et al (2006) Two-dimensional capillary liquid chromatography: pH Gradiention exchange and reversed phase chromatography for rapid separation of proteins. J Chromatogr A 1120:132-141
15. Shan L, Anderson DJ (2002) Gradient chromatofocusing. Versatile pH gradient separation of proteins in ion-exchange HPLC: characterization studies. Anal Chem 74:5641-5649
16. Wester field M (2000) The zebra fish book, A guide for the laboratory use of zebra fish (Danio rerio), 4th edn. University of Oregon Press, Eugene, OR