Chromatofocusing of peptides and proteins using linear pH gradients formed on strong ion-exchange adsorbents

Biotechnology and Bioengineering

Volumn 87, Issue 3, 2004, Pages 376-387

  Kang, Xuezhen ^a,b   Frey, Douglas D ^a  

^a UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

^b GENZYME CORPORATION   (United States)

Author keywords

Chromatofocusing; Ion exchange; Peptides; Proteins

Indexed keywords

CHROMATOGRAPHY; ION EXCHANGE; PH EFFECTS; PROTEINS; SEPARATION;

CHROMATOFOCUSING; PEPTIDES;

BIOTECHNOLOGY;

ADSORBENT; AMPHOLYTE; PEPTIDE; PROTEIN;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; CATION EXCHANGE; CHROMATOFOCUSING; CONCENTRATION RESPONSE; ION EXCHANGE; PH MEASUREMENT; TITRIMETRY;

ADSORPTION; CHROMATOGRAPHY, ION EXCHANGE; COMPUTER SIMULATION; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; HYDROGEN-ION CONCENTRATION; ION EXCHANGE RESINS; LINEAR MODELS; MODELS, CHEMICAL; PEPTIDES; PROTEIN BINDING; PROTEINS;

EID: 4143134324     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20122     Document Type: Article

References (30)

1. Alpert AJ. 1991. Ion-exchange high-performance liquid chromatography of peptides. In: Mant CT, Hodges RS, editors. HPLC of peptides and proteins: separation, analysis, and conformation, Boca Raton, FL: CRC Press. p 187-194.
2. Alpert AJ, Andrews PC. 1988. Cation-exchange chromatography of peptides on poly(2-sulfoethyl aspartamide)-silica. J Chromatogr 443:85-96.
3. Amersham Pharmacia Biotech. 1981. Chromatofocusing with Polybuffer and PBE. Uppsala, Sweden: Amersham.
4. Bates RC, Kang X, Frey DD. 2000. High performance chromatofocusing using linear and concave pH gradients formed with simple buffer mixtures. I. Effect of buffer composition on the gradient shape. J Chromatogr A 890:25-36.
5. Bjellqvist B, Hughes GJ, Pasquali C, Paquet N, Ravier F, Sanchez JC, Frutiger S, Hochstrasser D. 1993. The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. Electrophoresis 14:1023-1031.
6. Croft LR. 1980. Handbook of protein sequence analysis: a compilation of amino acid sequences of proteins with an introduction to the methodology. New York: Wiley.
7. Dizdaroglu M. 1985. Weak anion-exchange high-performance liquid chromatography of peptides. J Chromatogr 334:49-69.
8. Espinosa S, Bosch E, Roses M. 2000. Retention of ionizable compounds in HPLC. 5. pH scales and the retention of acids and bases with acetronitrile-water mobile phases. Anal Chem 72:5193-5200.
9. Fermo I, De Vecchi E, Diomede L, Paroni R. 1990. Serum amino acid analysis with pre-column derivatization: comparison of the o-phthaldialdehyde and N,N-diethyl-2,4-dinitro-5-fluoroaniline methods. J Chromatogr 534:23-36.
10. Frey DD, Barnes A, Strong JC. 1995. Numerical studies of multicomponent chromatography using pH gradients. AlChe J 41:1171-1183.
11. Frey DD, Narahari CR, Butler CD. 2002. General local-equilibrium chromatographic theory for eluents containing adsorbing buffers. AlChe J 48:561-571.
12. Gill SC, von Hippel PH. 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 182:319-326.
13. Gulati D, Bongers J, Burman S. 1999. RP-HPLC tryptic mapping of IgG1 proteins with post-column fluorescence derivatization. J Pharm Biomed Anal 21:887-893.
14. Isobe T, Takayasu T, Takai N, Okuyama T. 1982. High-performance liquid chromatography of peptides on a macroreticular cation-exchange resin: application to peptide mapping of Bence-Jones proteins. Anal Biochem 122:417-425.
15. Kang X. 2002. Novel methods of high-performance chromatofocusing. Ph.D. Dissertation, University of Maryland Baltimore County.
16. Kang X, Frey DD. 2002. Chromatofocusing using micropellicular column packings with computer-aided design of the elution buffer composition. Anal Chem 74:1038-1045.
17. Kang X, Frey DD. 2003. High-performance cation-exchange chromatofocusing of proteins. J Chromatogr A 991:117-128.
18. Liu H, Cho B, Strong R, Krull IS, Cohen S, Chan KC, Issaq HJ. 1999. Derivatization of peptides and small proteins for improved identification and detection in capillary zone electrophoresis. Anal Chim Acta 400:181-209.
19. Mant CT, Hodges RS. 1985. Separation of peptides by strong cation-exchange high-performance liquid chromatography. J Chromatogr 327:147-155.
20. Mant CT, Hodges RS. 1991. HPLC of peptides and proteins: separation, analysis and conformation. Boca Raton, FL: CRC Press.
21. Mantabe T. 1999. Capillary electrophoresis of proteins for proteomic studies. Electrophoresis 20:3116-3121.
22. Mazzeo JR, Martineau JA, Krull IS. 1993. Peptide mapping using EOF-Driven capillary isoelectric focusing. Anal Biochem 208:323-329.
23. Mohan D, Lee CS. 2002. Extension of separation range in capillary isoelectric focusing for resolving highly basic biomolecules. J Chromatogr A 979:271-276.
24. Schroeder WA, Robertson B. 1965. An improved gradient for ion exchange chromatography of peptides on Dowex-1. Anal Chem 37:1583-1585.
25. Shen Y, Berger SJ, Anderson GA, Smith RD. 2000. High-efficiency capillary isoelectric focusing of peptides. Anal Chem 72:2154-2159.
26. Sluyterman LAA, Elgersma O. 1978. Chromatofocusing: isoelectric focusing on ion-exchange columns. I. General principles. J Chromatogr 150:17-30.
27. Sluyterman LAA, Wijdenes J. 1978. Chromatofocusing: isoelectric focusing on ion-exchange columns. II. Experimental verification. J Chromatogr 150:31-44.
28. Stryer L. 1975. Biochemistry. San Francisco: W.H. Freeman.
29. Takahashi N, Takahashi Y, Putnam FW. 1983. Two-dimensional high-performance liquid chromatography and chemical modification in the strategy of sequence analysis: complete amino acid sequence of the lambda light chain of human immunoglobulin. J Chromatogr 266:511-522.
30. Wall RA. 1970. Separation of peptides on macroreticular ion exchangers. Anal Biochem 35:203-208.