Live cell imaging of chemotactic dendritic cell migration in explanted mouse ear preparations

Methods in Molecular Biology

Volumn 1013, Issue , 2013, Pages 215-226

  Weber, Michele ^a   Sixt, Michael ^a  

^a INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA   (Austria)

Author keywords

Cell migration; Chemotaxis; Dendritic cells; Dermis; Imaging; Interstitium; Lymphatic vessels; Skin

Indexed keywords

CHEMOKINE; FLUORESCENT DYE;

ANIMAL; ARTICLE; C57BL MOUSE; CELL CULTURE; CELL TRACKING; CHEMOTAXIS; COCULTURE; CONFOCAL MICROSCOPY; DENDRITIC CELL; EAR; IMAGE PROCESSING; IMMUNOLOGY; METABOLISM; METHODOLOGY; MOUSE; SIGNAL TRANSDUCTION; TIME; TISSUE CULTURE TECHNIQUE;

ANIMALS; CELL TRACKING; CELLS, CULTURED; CHEMOKINES; CHEMOTAXIS; COCULTURE TECHNIQUES; DENDRITIC CELLS; EAR; FLUORESCENT DYES; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MICE, INBRED C57BL; MICROSCOPY, CONFOCAL; SIGNAL TRANSDUCTION; TIME FACTORS; TISSUE CULTURE TECHNIQUES;

EID: 84886859740     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-426-5_14     Document Type: Article

References (8)

1. Nourshargh S, Hordijk PL, Sixt M (2010) Breaching multiple barriers: leukocyte motility through venular walls and the interstitium. Nat Rev Mol Cell Biol 11:366-378
2. Randolph GJ, Angeli V, Swartz MA (2005) Dendritic-cell traf ficking to lymph nodes through lymphatic vessels. Nat Rev Immunol 5:617-628
3. Lammermann T, Bader BL, Monkley et al (2008) Rapid leukocyte migration by integrin-independent fl owing and squeezing. Nature 453:51-55
4. P fl icke H, Sixt M (2009) Preformed portals facilitate dendritic cell entry into afferent lymphatic vessels. J Exp Med 206:2925-2935
5. Lammermann T, Renkawitz J, Wu X et al (2009) Cdc42-dependent leading edge coordination is essential for interstitial dendritic cell migration. Blood 113: 5703-5710
6. Menges M, Rössner S, Voigtländer C et al (2002) Repetitive injections of dendritic cells matured with tumor necrosis factor alpha induce antigen-speci fic protection of mice from autoimmunity. J Exp Med 195:15-21
7. Ohl L, Mohaupt M, Czeloth N et al (2004) CCR7 governs skin dendritic cell migration under in fl ammatory and steady-state conditions. Immunity 21:279-288
8. Price AA, Cumberbatch M, Kimber I et al (1997) Alpha 6 integrins are required for Langerhans cell migration from the epidermis. J Exp Med 186:1725-1735