Metabolic labeling and membrane fractionation for comparative proteomic analysis of Arabidopsis thaliana suspension cell cultures

Journal of Visualized Experiments

Volumn , Issue 79, 2013, Pages

  Szymanski, Witold G ^a   Kierszniowska, Sylwia ^a   Schulze, Waltraud X ^a,b  

^a Signaling Proteomics   (Germany)

^b UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

Arabidopsis; Arabidopsis thaliana; Cellular structures; Detergent resistant membranes (DRM); Genetically modified; Intracellular signaling peptides and proteins; Isotope labeling; Issue 79; Mass spectrometry; Membrane lipids; Membrane microdomains; Membrane proteins; Metabolic labeling; Plants; Plasma membrane fractionation; Proteomics; Quantitative proteomics; Stable isotope labeling; Suspension cell cultures; Two phase system

Indexed keywords

DETERGENT; NITRIC ACID DERIVATIVE; NITROGEN; POTASSIUM DERIVATIVE; POTASSIUM NITRATE; VEGETABLE PROTEIN; NITRATE;

ARABIDOPSIS; CELL FRACTIONATION; CELL MEMBRANE; CHEMISTRY; CULTURE TECHNIQUE; CYTOLOGY; METABOLISM; PROCEDURES; PROTEOMICS; ARTICLE; METHODOLOGY;

ARABIDOPSIS; CELL CULTURE TECHNIQUES; CELL FRACTIONATION; CELL MEMBRANE; DETERGENTS; MEMBRANE MICRODOMAINS; NITRATES; NITROGEN; PLANT PROTEINS; POTASSIUM COMPOUNDS; PROTEOMICS;

EID: 84886840624     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50535     Document Type: Article

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