Isolation of pulmonary Artery smooth muscle cells from neonatal mice

Journal of Visualized Experiments

Volumn , Issue 80, 2013, Pages

  Lee, Keng Jin ^a   Czech, Lyubov ^a   Waypa, Gregory B ^a   Farrow, Kathryn N ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Basic protocol; Cardiovascular abnormalities; CGMP; Development; Hypertension; Immunostaining; Issue 80; Muscle; Phosphodiesterases; Pulmonary; Pulmonary hypertension; Smooth; Vascular; Vascular smooth muscle

Indexed keywords

DESMIN; MYOSIN;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; CULTURE MEDIUM; CYTOLOGY; METHODOLOGY; MOUSE; MUSCLE CONTRACTION; MUSCLE RELAXATION; NEWBORN; PULMONARY ARTERY; VASCULAR SMOOTH MUSCLE;

ANIMALS; ANIMALS, NEWBORN; CATTLE; CULTURE MEDIA; CYTOLOGICAL TECHNIQUES; DESMIN; MICE; MUSCLE CONTRACTION; MUSCLE RELAXATION; MUSCLE, SMOOTH, VASCULAR; MYOSINS; PULMONARY ARTERY;

EID: 84886836936     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50889     Document Type: Article

References (27)

1. Levin, D.L., Rudolph, A.M., Heymann, M.A., & Phibbs, R.H. Morphological development of the pulmonary vascular bed in fetal lambs. Circulation. 53, 144-151 (1976).
2. Walsh-Sukys, M.C., et al. Persistent pulmonary hypertension of the newborn in the era before nitric oxide: practice variation and outcomes. Pediatrics. 105, 14-20 (2000).
3. Khemani, E., et al. Pulmonary artery hypertension in formerly premature infants with bronchopulmonary dysplasia: clinical features and outcomes in the surfactant era. Pediatrics. 120, 1260-1269 (2007).
4. Jobe, A.H. & Bancalari, E. Bronchopulmonary dysplasia. Am. J. Respir. Crit. Care Med. 163, 1723-1729 (2001).
5. Mourani, P.M., Sontag, M.K., Younoszai, A., Ivy, D.D., & Abman, S.H. Clinical utility of echocardiography for the diagnosis and management of pulmonary vascular disease in young children with chronic lung disease. Pediatrics. 121, 317-325 (2008).
6. Check, J., et al. Fetal growth restriction and pulmonary hypertension in premature infants with bronchopulmonary dysplasia. J. Perinatol. doi:10.1038/jp.2012.164 (2013).
7. Farrow, K.N., et al. Hyperoxia increases phosphodiesterase 5 expression and activity in ovine fetal pulmonary artery smooth muscle cells. Circ. Res. 102, 226-233 (2008).
8. Aschner, J.L., et al. Endothelial nitric oxide synthase gene transfer enhances dilation of newborn piglet pulmonary arteries. Am. J. Physiol. 277, H371-379 (1999).
9. Cornfield, D.N., Stevens, T., McMurtry, I.F., Abman, S.H., & Rodman, D.M. Acute hypoxia increases cytosolic calcium in fetal pulmonary artery smooth muscle cells. Am. J. Physiol. 265, L53-56 (1993).
10. Bailly, K., Ridley, A.J., Hall, S.M., & Haworth, S.G. RhoA activation by hypoxia in pulmonary arterial smooth muscle cells is age and site specific. Circ. Res. 94, 1383-1391, doi:10.1161/01.RES.0000128405.83582.2e (2004).
11. Black, S.M., DeVol, J.M., & Wedgwood, S. Regulation of fibroblast growth factor-2 expression in pulmonary arterial smooth muscle cells involves increased reactive oxygen species generation. Am. J. Physiol. Cell Physiol. 294, C345-354, doi:10.1152/ajpcell.00216.2007 (2008).
12. Cogolludo, A., Moreno, L., Lodi, F., Tamargo, J., & Perez-Vizcaino, F. Postnatal maturational shift from PKCzeta and voltage-gated K+ channels to RhoA/Rho kinase in pulmonary vasoconstriction. Cardiovasc. Res. 66, 84-93, doi:10.1016/j.cardiores.2004.12.019 (2005).
13. Wedgwood, S., et al. Hydrogen peroxide regulates extracellular superoxide dismutase activity and expression in neonatal pulmonary hypertension. Antiox. Redox Signal. 15, 1497-1506, doi:10.1089/ars.2010.3630 (2011).
14. Farrow, K.N., et al. Mitochondrial oxidant stress increases PDE5 activity in persistent pulmonary hypertension of the newborn. Respir. Physiol. Neurobiol. 174, 272-281 (2010).
15. Chester, M., et al. Cinaciguat, a soluble guanylate cyclase activator, augments cGMP after oxidative stress and causes pulmonary vasodilation in neonatal pulmonary hypertension. Am. J. Physiol. Lung Cell Mol. Physiol. 301, L755-764, doi:10.1152/ajplung.00138.2010 (2011).
16. Konduri, G.G., Bakhutashvili, I., Eis, A., & Gauthier, K.M. Impaired voltage gated potassium channel responses in a fetal lamb model of persistent pulmonary hypertension of the newborn. Pediatr. Res. 66, 289-294, doi:10.1203/PDR.0b013e3181b1bc89 (2009).
17. Olschewski, A., et al. Contribution of the K(Ca) channel to membrane potential and O2 sensitivity is decreased in an ovine PPHN model. Am. J. Physiol. Lung Cell Mol. Physiol. 283, L1103-1109, doi:10.1152/ajplung.00100.2002 (2002).
18. Aslam, M., et al. Bone marrow stromal cells attenuate lung injury in a murine model of neonatal chronic lung disease. Am. J. Respir. Crit. Care Med. 180, 1122-1130 (2009).
19. Balasubramaniam, V., et al. Bone marrow-derived angiogenic cells restore lung alveolar and vascular structure after neonatal hyperoxia in infant mice. Am. J. Physiol. Lung Cell Mol. Physiol. 298, L315-323 (2010).
20. de Visser, Y.P., et al. Phosphodiesterase-4 inhibition attenuates pulmonary inflammation in neonatal lung injury. Eur. Respir. J. 31, 633-644 (2008).
21. de Visser, Y.P., et al. Sildenafil attenuates pulmonary inflammation and fibrin deposition, mortality and right ventricular hypertrophy in neonatal hyperoxic lung injury. Respir. Res. 10, 30 (2009).
22. Ladha, F., et al. Sildenafil improves alveolar growth and pulmonary hypertension in hyperoxia-induced lung injury. Am. J. Respir. Crit. Care Med. 172, 750-756 (2005).
23. Farrow, K.N., et al. Brief hyperoxia increases mitochondrial oxidation and increases phosphodiesterase 5 activity in fetal pulmonary artery smooth muscle cells. Antioxid. Redox Signal. 17, 460-470, doi:10.1089/ars.2011.4184 (2012).
24. Waypa, G.B., et al. Hypoxia triggers subcellular compartmental redox signaling in vascular smooth muscle cells. Circ. Res. 106, 526-535 (2010).
25. Waypa, G.B., et al. Superoxide Generated at Mitochondrial Complex III Triggers Acute Responses to Hypoxia in the Pulmonary Circulation. Am. J. Respir. Crit. Care Med. 187, 424-432, doi:10.1164/rccm.201207-1294OC (2013).
26. Marshall, C., Mamary, A.J., Verhoeven, A.J., & Marshall, B.E. Pulmonary artery NADPH-oxidase is activated in hypoxic pulmonary vasoconstriction. Am. J. Respir. Cell Mol. Biol. 15, 633-644 (1996).
27. Farrow, K.N., et al. Superoxide Dismutase and Inhaled Nitric Oxide Normalize Phosphodiesterase 5 Expression and Activity in Neonatal Lambs with Persistent Pulmonary Hypertension. Am. J. Physiol. Lung Cell Mol. Physiol. 299, L109-116 (2010).