An optimized technique for rapid genome modifications of Bacillus subtilis

Journal of Microbiological Methods

Volumn 95, Issue 3, 2013, Pages 350-352

  Kumpfmüller, Jana ^a   Kabisch, Johannes ^a   Schweder, Thomas ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Bacillus subtilis; Gene library; Natural competence; Six sites; Transformation efficiency; Wild type strain

Indexed keywords

BETA RECOMBINASE; RECOMBINASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; BACTERIAL GENOME; BACTERIUM COMPETENCE; CONTROLLED STUDY; CRE LOX SYSTEM; DNA STRUCTURE; GENE CASSETTE; GENE MUTATION; GENETIC ANALYSIS; GENETIC ENGINEERING; GENETIC MARKER; GENETIC TRANSFORMATION; GENETIC VARIABILITY; MARKER GENE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION;

BACILLUS SUBTILIS; BASE PAIRS; BP; CAA; CASAMINO ACIDS; CFU; COLONY FORMING UNITS; GENE LIBRARY; NATURAL COMPETENCE; OD; OPTICAL DENSITY; PCR; POLYMERASE CHAIN REACTION; SIX SITES; SIX-SITE-SPECTINOMYCIN RESISTANCE GENE; SSS; TM; TRANSFORMATION EFFICIENCY; TRANSFORMATION MEDIUM; WILD TYPE STRAIN;

BACILLUS SUBTILIS; GENE KNOCK-IN TECHNIQUES; GENE KNOCKOUT TECHNIQUES; GENETICS, MICROBIAL; TRANSFORMATION, BACTERIAL;

EID: 84886814795     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.10.003     Document Type: Article

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