Aspartic acid 397 in subunit B of the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae forms part of a sodium-binding site, is involved in cation selectivity, and affects cation-binding site cooperativity

Journal of Biological Chemistry

Volumn 288, Issue 43, 2013, Pages 31241-31249

  Shea, Michael E ^a   Juárez, Oscar ^a   Cho, Jonathan ^a   Barquera, Blanca ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID RESIDUES; ASPARTIC ACIDS; CATION SELECTIVITY; ELECTRON-TRANSFER STEP; MUTANT ENZYMES; OXIDOREDUCTASES; PATHOGENIC BACTERIUM; VIBRIO CHOLERAE;

BINDING SITES; CELL MEMBRANES; CYTOLOGY; ENZYMES; METAL IONS; POSITIVE IONS; PUMPS;

AMINO ACIDS;

ARGININE; ASPARTIC ACID; CATION; CYSTEINE; GLUTAMIC ACID; LYSINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); SERINE; SODIUM ION; UBIQUINONE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BINDING SITE; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME PURIFICATION; MUTAGENESIS; MUTANT; NONHUMAN; PRIORITY JOURNAL; STEADY STATE; VIBRIO CHOLERAE;

BACTERIA; BACTERIAL METABOLISM; BIOENERGETICS-ELECTRON TRANSFER COMPLEX; REDOX; SODIUM TRANSPORT;

AMINO ACID SUBSTITUTION; ASPARTIC ACID; BACTERIAL PROTEINS; BINDING SITES; ELECTRON TRANSPORT; ION TRANSPORT; MUTATION, MISSENSE; QUINONE REDUCTASES; SODIUM; VIBRIO CHOLERAE;

EID: 84886680239     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.510776     Document Type: Article

References (19)

1. +-translocating NADH:quinone oxidoreductase from Vibrio cholerae.. Biochemistry 41, 3781-3789
2. +-translocating NADH-quinone reductase from the marine Vibrio alginolyticus.. Biochim. Biophys. Acta 1505, 37-44
3. +- translocating NADH: quinone oxidoreductase: progress achieved and prospects of investigations.. Biochemistry 70, 143-149
4. + inhibition.. Eur. J. Biochem. 249, 770-776
5. +-coupled oxidative phosphorylation in Vibrio alginolyticus cells. Biochim. Biophys. Acta 850, 458-465
6. Dashper, S. G., Brownfield, L., Slakeski, N, Zilm, P. S., Rogers, A. H., and Reynolds, E. C. (2001) Sodium ion-driven serine/threonine transport in Porphyromonas gingivalis. J. Bacteriol. 183, 4142-4148
7. + motive force. J Bacteriol. 181, 1927-1930
8. +-pumping NADH:quinone oxidoreductase from Vibrio cholerae by PhoA-green fluorescent protein fusion analysis.. J. Bacteriol. 188, 8343-8351
9. +-translocating NADH:ubiquinone oxidoreductase catalytic cycle resolved by the ultrafast freeze-quench approach. J. Biol. Chem. 284, 5533-5538
10. +-pumping NADH:quinone oxidoreductase from Vibrio cholerae.. J. Biol. Chem. 284, 8963-8972
11. +-pumping NADH:quinone oxidoreductase from Vibrio cholerae.. Proc. Natl. Acad. Sci. U. S. A. 107, 12505-12510
12. +-pumping NADH:quinone oxidoreductase from Vibrio cholerae.. Biochemistry 51, 4072-4077
13. +-NQR) from Vibrio cholerae involved in sodium translocation.. Biochemistry 48, 9516-9524
14. +-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae.. Biochemistry 43, 12322-12330
15. Roitel, O., Sergienko, E., and Branlant, G. (1999) Dimers generated from tetrameric phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus are inactive but exhibit cooperativity in NAD binding.. Biochemistry 38, 16084-16091
16. +-pumping NADH:quinone oxidoreductase from Vibrio cholerae.. J. Biol. Chem. 286, 26383-26390
17. +-pumping NADH:quinone oxidoreductase from Vibrio cholerae.. Biochemistry 52, 3085-3093
18. + bioenergetics in Acetobacterium woodii. Biochim. Biophys. Acta 1787, 691-696
19. Gouaux, E., and Mackinnon, R. (2005) Principles of selective ion transport in channels and pumps. Science 310, 1461-1465