Mutagenesis study of the 2Fe-2S center and the FAD binding site of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae

Biochemistry

Volumn 43, Issue 38, 2004, Pages 12322-12330

  Barquera, Blanca ^a,c   Nilges, Mark J ^b   Morgan, Joel E ^a   Ramirez Silva, Leticia ^a,d   Zhou, Weidong ^a   Gennis, Robert B ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b Illinois EPR Research Center   (United States)

^c RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^d UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTRONS; ENZYMES; MUTAGENESIS; OXIDATION;

FLAVIN BINDING DOMAIN; PATHOGENIC BACTERIA;

BACTERIA;

ANION; ARGININE; CYSTEINE; DITHIONITE; FERRICYANIDE; FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE; IRON SULFUR PROTEIN; MUTANT PROTEIN; QUERCETIN; RADICAL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); RIBOFLAVIN; SEMIQUINONE; SERINE; SODIUM ION; TYROSINE; UBIQUINONE;

AEROBIC METABOLISM; AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CONTROLLED STUDY; COVALENT BOND; ELECTROCHEMISTRY; ELECTRON SPIN RESONANCE; ELECTRON TRANSPORT; ENZYME SUBUNIT; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS; SODIUM TRANSPORT; STEADY STATE; SURFACE CHARGE; VIBRIO CHOLERAE;

AMINO ACID SEQUENCE; BINDING SITES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTRON TRANSPORT COMPLEX I; FLAVIN-ADENINE DINUCLEOTIDE; IRON; LIGANDS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; SEQUENCE ALIGNMENT; SODIUM; SPECTRUM ANALYSIS; SULFUR; VIBRIO CHOLERAE;

NEGIBACTERIA; VIBRIO; VIBRIO CHOLERAE;

EID: 4644279888     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi048689y     Document Type: Article

References (27)

1. +-dependent amino acid transport in moderately halophilic bacteria, J. Bioenerg. Biomembr. 25, 385-391.
2. Dimroth, P. (1997) Primary sodium ion translocating enzymes, Biochim. Biophys. Acta 1318, 11-51.
3. +-translocating NADH-quinone reductase from the marine Vibrio alginolyticus, Biochim. Biophys. Acta 1505, 37-44.
4. +-dependent motility and modes of membrane energization in the marine alkalotolerant Vibrio alginolyticus, Biochim. Biophys. Acta 850, 449-457.
5. +-coupled oxidative phosphorylation in Vibrio alginolyticus cells, Biochim. Biophys. Acta 850, 458-465.
6. +-translocating NADH-quinone reductase in Haemophilus influenzae, FEBS Lett. 381, 174-176.
7. +-translocating NADH:ubiquinone oxidoreductase from Vibrio harveyi, Biochemistry 38, 16246-16252.
8. + pump from Vibrio cholerae with covalently attached FMN, FEBS Lett. 492, 45-49.
9. +-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus, FEBS Lett. 356, 333-338.
10. +-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli, FEBS Lett. 356, 330-332.
11. +-translocating NADH-quinone reductase from the marine Vibrio alginolyticus, FEBS Lett. 422, 240-242.
12. Hayashi, M., and Unemoto, T. (1986) FAD and FMN flavoproteins participate in the sodium-transport respiratory chain NADH:quinone reductase of a marine bacterium, FEBS Lett. 202, 327-329.
13. + pump, Biochemistry 35, 6233-6242.
14. +-translocating NADH-quinone reductase from Vibrio alginolyticus, FEBS Lett. 23723, 1-4.
15. +-translocating NADH-quinone reductase from Vibrio alginolyticus, FEBS Lett. 24493, 1-4.
16. Rich, P. R., Meunier, B., and Ward, F. B. (1995) Predicted structure and possible ion motive mechanism of the sodium-linked NADH-ubiquinone oxidoreductase of Vibrio alginolyticus, FEBS Lett. 375, 5-10.
17. Corpet, F. (1988) Multiple sequence alignment with hierarchical clustering, Nucleic Acids Res. 16, 10881-10890.
18. Smith, R. F., Wiese, B. A., Wojzynski, M. K., Davison, D. B., and Worley, K. C. (1996) BCM search launcher- an integrated interface to molecular biology data base search and analysis services available on the World Wide Web, Genome Res. 6, 454-462.
19. Nishida, H., Inaka, K., and Miki, K. (1995) Specific arrangement of three amino acid residues for flavin-binding barrel structures in NADH-cytochrome b5 reductase and the other flavin-dependent reductases, FEBS Lett. 361, 97-100.
20. +-motive NADH: Quinone oxidoreductase in Vibrio alginolyticus, FEBS Lett. 409, 475-477.
21. +-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species, Eur. J. Biochem. 269, 1287-1292.
22. +-translocating NADH:quinone oxidoreductase from Vibrio cholerae, J. Am. Chem. Soc. 125, 265-275.
23. +-motive NADH:quinone oxidoreductase from Vibrio harveyi, Biochim. Biophys. Acta 1556, 113-120.
24. +-translocating NADH:quinone oxidoreductase from Vibrio cholerae, Biochemistry 41, 3781-3789.
25. +-pumping NADH:quinone oxidoreductase from Vibrio cholerae, Proc. Natl. Acad. Sci. U.S.A. 99, 10322-10324.
26. + is translocated at NADH: quinone oxidoreductase segment in the respiratory chain of Vibrio alginolylicus, J. Biol. Chem. 259, 7785-7790.
27. Aliverti, A., Hagen, W. R., and Zanetti, G. (1995) Direct electrochemistry and EPR spectroscopy of spinach ferredoxin mutants with modified electron transfer properties, FEBS Lett. 368, 220-224.