Live imaging of transgenic mice expressing FRET biosensors

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS

Volumn , Issue , 2013, Pages 125-128

  Kamioka, Yuji ^a   Sumiyama, Kenta ^b   Mizuno, Rei ^a   Matsuda, Michiyuki ^a  

^a KYOTO UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF GENETICS   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE IMAGING; FLUORESCENT PROTEIN; FUNCTIONAL BIOSENSORS; GENETIC BACKGROUNDS; RESONANCE ENERGY TRANSFER; SIGNAL-TO-BACKGROUND RATIO; SPATIO-TEMPORAL DYNAMICS; TWO-PHOTON EXCITATION MICROSCOPIES;

DIAGNOSIS; ENERGY TRANSFER; FLUORESCENCE; MAMMALS; PLANTS (BOTANY); SIGNAL TRANSDUCTION;

BIOSENSORS;

BENZAMIDE DERIVATIVE; BENZOIC ACID DERIVATIVE; BUCLADESINE; CYCLIC AMP DEPENDENT PROTEIN KINASE; DIPHENYLAMINE; ETALOCIB; FLUORESCENT DYE; MITOGEN ACTIVATED PROTEIN KINASE; N (2,3 DIHYDROXYPROPOXY) 3,4 DIFLUORO 2 (2 FLUORO 4 IODOANILINO)BENZAMIDE; THEOPHYLLINE;

ANALOGS AND DERIVATIVES; ANIMAL; CELL MOTION; CHEMISTRY; DRUG EFFECTS; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC PROCEDURES; GENETICS; GRANULOCYTE; METABOLISM; MOUSE; PATHOLOGY; PLASMID; SIGNAL TRANSDUCTION; SKIN; SMALL INTESTINE; TIME LAPSE IMAGING; TRANSGENIC MOUSE;

ANIMALS; BENZAMIDES; BENZOATES; BIOSENSING TECHNIQUES; BUCLADESINE; CELL MOVEMENT; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DIPHENYLAMINE; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; GRANULOCYTES; INTESTINE, SMALL; MICE; MICE, TRANSGENIC; MICROSCOPY, FLUORESCENCE; PLASMIDS; SIGNAL TRANSDUCTION; SKIN; THEOPHYLLINE; TIME-LAPSE IMAGING;

EID: 84886515625     PISSN: 1557170X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/EMBC.2013.6609453     Document Type: Conference Paper

References (20)

1. E. A. Jares-Erijman and T. M. Jovin, "Imaging molecular interactions in living cells by FRET microscopy, " Curr Opin Chem Biol, vol. 10, pp. 409-16, Oct 2006.
2. L. Hodgson, O. Pertz, and K. M. Hahn, "Design and optimization of genetically encoded fluorescent biosensors: GTPase biosensors, " Methods Cell Biol, vol. 85, pp. 63-81, 2008.
3. L. Hodgson, O. Pertz, and K. M. Hahn, "Design and optimization of genetically encoded fluorescent biosensors: GTPase biosensors, " Methods Cell Biol, vol. 85, pp. 63-81, 2008.
4. C. Depry and J. Zhang, "Visualization of kinase activity with FRET-based activity biosensors, " Curr Protoc Mol Biol, vol. Chapter 18, p. Unit 18 15, Jul 2010.
5. E. Kiyokawa, K. Aoki, T. Nakamura, and M. Matsuda, "Spatiotemporal Regulation of Small GTPases as Revealed by PIEErobes Based on the Principle of Forster Resonance Energy Transfer (FRET): Implications for Signaling and Pharmacology, " Annual Review of Pharmacology and Toxicology, Vol 51, 2011, vol. 51, pp. 337-358, 2011.
6. K. Aoki, E. Kiyokawa, T. Nakamura, and M. Matsuda, "Visualization of growth signal transduction cascades in living cells with genetically encoded probes based on Forster resonance energy transfer, " Philosophical Transactions of the Royal Society B-Biological Sciences, vol. 363, pp. 2143-2151, Jun 27 2008.
7. K. Aoki, N. Komatsu, E. Hirata, Y. Kamioka, and M. Matsuda, "Stable expression of FRET biosensors: a new light in cancer research, " Cancer Sci, vol. 103, pp. 614-9, Apr 2012.
8. D. Calebiro, V. O. Nikolaev, M. C. Gagliani, T. de Filippis, C. Dees, C. Tacchetti, L. Persani, and M. J. Lohse, "Persistent cAMP-Signals Triggered by Internalized G-Protein-Coupled Receptors, " Plos Biology, vol. 7, Aug 2009.
9. M. Hara, V. Bindokas, J. P. Lopez, K. Kaihara, L. R. Landa Jr., M. Harbeck, and M. W. Roe, "Imaging endoplasmic reticulum calcium with a fluorescent biosensor in transgenic mice, " Am J Physiol Cell Physiol, vol. 287, pp. C932-8, Oct 2004.
10. E. Isotani, G. Zhi, K. S. Lau, J. Huang, Y. Mizuno, A. Persechini, R. Geguchadze, K. E. Kamm, and J. T. Stull, "Real-time evaluation of myosin light chain kinase activation in smooth muscle tissues from a transgenic calmodulin-biosensor mouse, " Proc Natl Acad Sci U S A, vol. 101, pp. 6279-84, Apr 20 2004.
11. V. O. Nikolaev, M. Bunemann, E. Schmitteckert, M. J. Lohse, and S. Engelhardt, "Cyclic AMP imaging in adult cardiac myocytes reveals far-reaching beta1-adrenergic but locally confined beta2-adrenergic receptor-mediated signaling, " Circ Res, vol. 99, pp. 1084-91, Nov 10 2006.
12. S. D. Atkin, S. Patel, A. Kocharyan, L. A. Holtzclaw, S. H. Weerth, V. Schram, J. Pickel, and J. T. Russell, "Transgenic mice expressing a cameleon fluorescent Ca2+ indicator in astrocytes and Schwann cells allow study of glial cell Ca2+ signals in situ and in vivo, " J Neurosci Methods, vol. 181, pp. 212-26, Jul 30 2009.
13. Y. Yamaguchi, N. Shinotsuka, K. Nonomura, K. Takemoto, K. Kuida, H. Yosida, and M. Miura, "Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure, " J Cell Biol, vol. 195, pp. 1047-60, Dec 12 2011.
14. Y. Kamioka, K. Sumiyama, R. Mizuno, Y. Sakai, E. Hirata, E. Kiyokawa, and M. Matsuda, "Live imaging of protein kinase activities in transgenic mice expressing FRET biosensors, " Cell Struct Funct, vol. 37, pp. 65-73, 2012.
15. K. Sumiyama, K. Kawakami, and K. Yagita, "A simple and highly efficient transgenesis method in mice with the Tol2 transposon system and cytoplasmic microinjection, " Genomics, vol. 95, pp. 306-11, May 2010.
16. N. Komatsu, K. Aoki, M. Yamada, H. Yukinaga, Y. Fujita, Y. Kamioka, and M. Matsuda, "Development of an optimized backbone of FRET biosensors for kinases and GTPases, " Mol Biol Cell, vol. 22, pp. 4647-56, Dec 2011.
17. C. D. Harvey, A. G. Ehrhardt, C. Cellurale, H. Zhong, R. Yasuda, R. J. Davis, and K. Svoboda, "A genetically encoded fluorescent sensor of ERK activity, " Proc Natl Acad Sci U S A, vol. 105, pp. 19264-9, Dec 9 2008.
18. M. E. Bianchi, "DAMPs, PAMPs and alarmins: all we need to know about danger, " Journal of Leukocyte Biology, vol. 81, pp. 1-5, Jan 2007.
19. A. M. Piccinini and K. S. Midwood, "DAMPening Inflammation by Modulating TLR Signalling, " Mediators of Inflammation, 2010.
20. R. K. Sivamani, C. E. Pullar, C. G. Manabat-Hidalgo, D. M. Rocke, R. C. Carlsen, D. G. Greenhalgh, and R. R. Isseroff, "Stress-mediated increases in systemic and local epinephrine impair skin wound healing: potential new indication for beta blockers, " PLoS Med, vol. 6, p. e12, Jan 13 2009.