Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells

PLoS ONE

Volumn 8, Issue 10, 2013, Pages

  Stagge, Franziska ^a,b   Mitronova, Gyuzel Y ^a   Belov, Vladimir N ^a   Wurm, Christian A ^a,b   Jakobs, Stefan ^a,b,c  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)   (Germany)

^c UNIVERSITY MEDICAL CENTER GÖTTINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CLIP PROTEIN; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; HALO TAG PROTEIN; HYBRID PROTEIN; RHODAMINE; SNAP PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL BUDDING; CELL LABELING; CELL VIABILITY; ELECTROPORATION; ISOMER; MICROSCOPY; NONHUMAN; PROTEIN EXPRESSION; PROTON NUCLEAR MAGNETIC RESONANCE; RELIABILITY; SACCHAROMYCES CEREVISIAE; YEAST CELL;

CELL SURVIVAL; COLOR; ELECTROPORATION; FLUORESCEIN; ISOMERISM; MICROSCOPY; RECOMBINANT FUSION PROTEINS; RHODAMINES; SACCHAROMYCETALES; STAINING AND LABELING;

EID: 84886440251     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0078745     Document Type: Article

References (29)

1. Fernández-Suárez M, Ting AY, (2008) Fluorescent probes for super-resolution imaging in living cells. Nat Rev Mol Cell Biol 9: 929-943. doi:10.1038/nrm2531. PubMed: 19002208.
2. Hinner MJ, Johnsson K, (2010) How to obtain labeled proteins and what to do with them. Curr Opin Biotechnol 21: 766-776. doi:10.1016/j.copbio.2010.09.011. PubMed: 21030243.
3. Keppler A, Gendreizig S, Gronemeyer T, Pick H, Vogel H, et al. (2003) A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol 21: 86-89. PubMed: 12469133.
4. Gautier A, Juillerat A, Heinis C, Corrêa IR Jr., Kindermann M, et al. (2008) An engineered protein tag for multiprotein labeling in living cells. Chem Biol 15: 128-136. doi:10.1016/j.chembiol.2008.01.007. PubMed: 18291317.
5. Los GV, Encell LP, McDougall MG, Hartzell DD, Karassina N, et al. (2008) HaloTag: a novel protein labeling technology for cell imaging and protein analysis. ACS Chem Biol 3: 373-382. doi:10.1021/cb800025k. PubMed: 18533659.
6. Hein B, Willig KI, Wurm CA, Westphal V, Jakobs S, et al. (2010) Stimulated emission depletion nanoscopy of living cells using SNAP-tag fusion proteins. Biophys J 98: 158-163. doi:10.1016/j.bpj.2009.12.847. PubMed: 20074516.
7. Muster B, Kohl W, Wittig I, Strecker V, Joos F, et al. (2010) Respiratory chain complexes in dynamic mitochondria display a patchy distribution in life cells. PLOS ONE 5: e11910. doi:10.1371/journal.pone.0011910. PubMed: 20689601.
8. Srikun D, Albers AE, Nam CI, Iavarone AT, Chang CJ, (2010) Organelle-targetable fluorescent probes for imaging hydrogen peroxide in living cells via SNAP-Tag protein labeling. J Am Chem Soc 132: 4455-4465. doi:10.1021/ja100117u. PubMed: 20201528.
9. Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425: 686-691. doi:10.1038/nature02026. PubMed: 14562095.
10. Scherrer R, Louden L, Gerhardt P, (1974) Porosity of the yeast cell wall and membrane. J Bacteriol 118: 534-540. PubMed: 4597447.
11. Paumi CM, Chuk M, Snider J, Stagljar I, Michaelis S, (2009) ABC transporters in Saccharomyces cerevisiae and their interactors: new technology advances the biology of the ABCC (MRP) subfamily. Microbiol Mol Biol Rev 73: 577-593. doi:10.1128/MMBR.00020-09. PubMed: 19946134.
12. McMurray MA, Thorner J, (2008) Septin stability and recycling during dynamic structural transitions in cell division and development. Curr Biol 18: 1203-1208. doi:10.1016/j.cub.2008.07.020. PubMed: 18701287.
13. Chidley C, Haruki H, Pedersen MG, Muller E, Johnsson K, (2011) A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis. Nat Chem Biol 7: 375-383. doi:10.1038/nchembio.557. PubMed: 21499265.
14. Keppler A, Pick H, Arrivoli C, Vogel H, Johnsson K, (2004) Labeling of fusion proteins with synthetic fluorophores in live cells. Proc Natl Acad Sci U S A 101: 9955-9959. doi:10.1073/pnas.0401923101. PubMed: 15226507.
15. Jakobs S, Andresen M, Wurm CA, (2008) FlAsHMiller LW, eds., protein labeling. Weinheim: Wiley-VCH Verlag. Probes and tags to Study biomolecular function pp. pp. 73-88.
16. Wurm CA, Suppanz IE, Stoldt S, Jakobs S, (2010) Rapid FlAsH labelling in the budding yeast Saccharomyces cerevisiae. J Microsc 240: 6-13. doi:10.1111/j.1365-2818.2010.03378.x. PubMed: 21050208.
17. Griffin BA, Adams SR, Tsien RY, (1998) Specific covalent labeling of recombinant protein molecules inside live cells. Science 281: 269-272. doi:10.1126/science.281.5374.269. PubMed: 9657724.
18. Lukinavičius G, Umezawa K, Olivier N, Honigmann A, Yang G, et al. (2013) A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nat Chem 5: 132-139. doi:10.1038/nchem.1546. PubMed: 23344448.
19. Jones SA, Shim SH, He J, Zhuang X, (2011) Fast, three-dimensional super-resolution imaging of live cells. Nat Methods 8: 499-505. doi:10.1038/nmeth.1605. PubMed: 21552254.
20. Klein T, Löschberger A, Proppert S, Wolter S, van de Linde S, et al. (2011) Live-cell dSTORM with SNAP-tag fusion proteins. Nat Methods 8: 7-9. doi:10.1038/nmeth0111-7b. PubMed: 21191367.
21. Pellett PA, Sun X, Gould TJ, Rothman JE, Xu MQ, et al. (2011) Two-color STED microscopy in living cells Biomed Opt Express 2: 2364-2371. PubMed: 21833373.
22. Wilmes S, Staufenbiel M, Lisse D, Richter CP, Beutel O, et al. (2012) Triple-color super-resolution imaging of live cells: resolving submicroscopic receptor organization in the plasma membrane. Angew Chem Int Ed Engl 51: 4868-4871. doi:10.1002/anie.201200853. PubMed: 22488831.
23. Testa I, Wurm CA, Medda R, Rothermel E, von Middendorf C, et al. (2010) Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength. Biophys J 99: 2686-2694. doi:10.1016/j.bpj.2010.08.012. PubMed: 20959110.
24. Hell SW, (2009) Far-Field Optical Nanoscopy. In: Gräslund A, Rigler R, Widengren J, eds. Single molecule spectroscopy in chemistry, physics and biology. Berlin: Springer Verlag. pp. pp. 298-365.
25. Walther TC, Brickner JH, Aguilar PS, Bernales S, Pantoja C, et al. (2006) Eisosomes mark static sites of endocytosis. Nature 439: 998-1003. doi:10.1038/nature04472. PubMed: 16496001.
26. Neumann D, Bückers J, Kastrup L, Hell SW, Jakobs S, (2010) Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms. PMC Biophys 3: 4. doi:10.1186/1757-5036-3-4. PubMed: 20205711.
27. Westermann B, Neupert W, (2000) Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae. Yeast 16: 1421-1427. doi:10.1002/1097-0061(200011)16:15. PubMed: 11054823.
28. Andresen M, Schmitz-Salue R, Jakobs S, (2004) Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging. Mol Cell Biol 15: 5616-5622. doi:10.1091/mbc.E04-06-0454. PubMed: 15469986.
29. Sherman F, (2002) Getting started with yeast. In: Guthrie C, Fink GR, eds. Methods in enzymology guide to yeast genetics and molecular and cell biology- Part B London: Academic Press. vol. 350. pp.: 3-41.