Optimization, validation, and application of a real-time PCR protocol for quantification of viable bacterial cells in municipal sewage sludge and biosolids using reporter genes and Escherichia coli

Journal of Industrial Microbiology and Biotechnology

Volumn 40, Issue 11, 2013, Pages 1251-1261

  Van Frankenhuyzen, Jessica K ^a   Trevors, Jack T ^a   Flemming, Cecily A ^b   Lee, Hung ^a   Habash, Marc B ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b ONTARIO MINISTRY OF THE ENVIRONMENT   (Canada)

Author keywords

Biosolids; Escherichia coli; Propidium monoazide; Real time PCR; Reporter gene; Viable pathogen

Indexed keywords

AGRICULTURAL LAND; ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; BIOSOLID; CELL CULTURE; CELL DENSITY; CLINICAL PROTOCOL; DNA EXTRACTION; ESCHERICHIA COLI; GOVERNMENT REGULATION; MICROORGANISM DETECTION; NONHUMAN; PLATE COUNT; PROCESS OPTIMIZATION; PSEUDOMONAS; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; RHIZOBIUM RADIOBACTER; SLUDGE; WASTE WATER;

AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS SP.;

AGROBACTERIUM TUMEFACIENS; AZIDES; COLORING AGENTS; DNA, BACTERIAL; ESCHERICHIA COLI; EXTRACELLULAR SPACE; GENES, REPORTER; GENOME, BACTERIAL; MICROBIAL VIABILITY; PROPIDIUM; PSEUDOMONAS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SEWAGE;

EID: 84886289164     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-013-1319-x     Document Type: Article

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