Comparison of gene expression profiles in primary and immortalized human pterygium fibroblast cells

Experimental Cell Research

Volumn 319, Issue 18, 2013, Pages 2781-2789

  Hou, Aihua ^a   Voorhoeve, P Mathijs ^b,c   Lan, Wanwen ^a   Tin, Minqi ^a   Tong, Louis ^a,b,d,e  

^a SINGAPORE EYE RESEARCH INSTITUTE   (Singapore)

^b DUKE NUS MEDICAL SCHOOL   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d SINGAPORE NATIONAL EYE CENTRE   (Singapore)

^e NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Eye; Immortalization; Pterygium fibroblast

Indexed keywords

CLAUDIN 1; COLLAGEN TYPE 6; GREEN FLUORESCENT PROTEIN; HISTONE H2B; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE 2; PROTEIN P16; PROTEIN P53; SHORT HAIRPIN RNA; TELOMERASE REVERSE TRANSCRIPTASE;

ARTICLE; CELL AGING; CELL CYCLE M PHASE; CELL DIVISION; CELL IMMORTALIZATION; CELL NUCLEUS; CELL STRUCTURE; COMPARATIVE STUDY; CYTOPLASM; DOWN REGULATION; EMBRYO; EXPRESSION VECTOR; FIBROBLAST; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; MITOSIS; PRIMARY CELL CULTURE; PRIORITY JOURNAL; PTERYGIUM; SENESCENCE; UPREGULATION;

EID: 84886286727     PISSN: 00144827     EISSN: 10902422     Source Type: Journal    
DOI: 10.1016/j.yexcr.2013.08.022     Document Type: Article

References (37)

1. Chui J., Di Girolamo N., Wakefield D., Coroneo M.T. The pathogenesis of pterygium: current concepts and their therapeutic implications. Ocul. Surf. 2008, 6:24-43.
2. Di Girolamo N., Chui J., Coroneo M.T., Wakefield D. Pathogenesis of pterygia: role of cytokines, growth factors, and matrix metalloproteinases. Prog. Retin. Eye Res. 2004, 23:195-228.
3. Maini R., Collison D.J., Maidment J.M., Davies P.D., Wormstone I.M. Pterygial derived fibroblasts express functionally active histamine and epidermal growth factor receptors. Exp. Eye Res. 2002, 74:237-244.
4. Brenneisen P., Sies H., Scharffetter-Kochanek K. Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann. N Y Acad. Sci. 2002, 973:31-43.
5. Solomon A., Grueterich M., Li D.Q., Meller D., Lee S.B., Tseng S.C. Overexpression of Insulin-like growth factor-binding protein-2 in pterygium body fibroblasts. Invest. Ophthalmol. Vis. Sci. 2003, 44:573-580.
6. Nolan T.M., Di Girolamo N., Coroneo M.T., Wakefield D. Proliferative effects of heparin-binding epidermal growth factor-like growth factor on pterygium epithelial cells and fibroblasts. Invest. Ophthalmol. Vis. Sci. 2004, 45:110-113.
7. Riau A.K., Wong T.T., Beuerman R.W., Tong L. Calcium-binding S100 protein expression in pterygium. Mol. Vis. 2009, 15:335-342.
8. Kato N., Shimmura S., Kawakita T., Miyashita H., Ogawa Y., Yoshida S., Higa K., Okano H., Tsubota K. Beta-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium. Invest. Ophthalmol. Vis. Sci. 2007, 48:1511-1517.
9. Wong Y.W., Chew J., Yang H., Tan D.T., Beuerman R. Expression of insulin-like growth factor binding protein-3 in pterygium tissue. Br. J. Ophthalmol. 2006, 90:769-772.
10. Cox C.A., Amaral J., Salloum R., Guedez L., Reid T.W., Jaworski C., John-Aryankalayil M., Freedman K.A., Campos M.M., Martinez A., Becerra S.P., Carper D.A. Doxycycline's effect on ocular angiogenesis: an in vivo analysis. Ophthalmology 2010, 117:1782-1791.
11. Di Girolamo N., Wakefield D., Coroneo M.T. Doxycycline's and ocular angiogenesis. Ophthalmology 2011, 118:789-790. (author reply 790-781).
12. Di Girolamo N., Coroneo M.T., Wakefield D. UVB-elicited induction of MMP-1 expression in human ocular surface epithelial cells is mediated through the ERK1/2 MAPK-dependent pathway. Invest. Ophthalmol. Vis. Sci. 2003, 44:4705-4714.
13. Di Girolamo N., Wakefield D., Coroneo M.T. Differential expression of matrix metalloproteinases and their tissue inhibitors at the advancing pterygium head. Invest. Ophthalmol. Vis. Sci. 2000, 41:4142-4149.
14. Yang S.F., Lin C.Y., Yang P.Y., Chao S.C., Ye Y.Z., Hu D.N. Increased expression of gelatinase (MMP-2 and MMP-9) in pterygia and pterygium fibroblasts with disease progression and activation of protein kinase C. Invest. Ophthalmol. Vis. Sci. 2009, 50:4588-4596.
15. Tsai Y.Y., Chiang C.C., Yeh K.T., Lee H., Cheng Y.W. Effect of TIMP-1 and MMP in pterygium invasion. Invest. Ophthalmol. Vis. Sci. 2010, 51:3462-3467.
16. Di Girolamo N., Kumar R.K., Coroneo M.T., Wakefield D. UVB-mediated induction of interleukin-6 and -8 in pterygia and cultured human pterygium epithelial cells. Invest. Ophthalmol. Vis. Sci. 2002, 43:3430-3437.
17. Di Girolamo N., Wakefield D., Coroneo M.T. UVB-mediated induction of cytokines and growth factors in pterygium epithelial cells involves cell surface receptors and intracellular signaling. Invest. Ophthalmol. Vis. Sci. 2006, 47:2430-2437.
18. Di Girolamo N., Coroneo M., Wakefield D. Epidermal growth factor receptor signaling is partially responsible for the increased matrix metalloproteinase-1 expression in ocular epithelial cells after UVB radiation. Am. J. Pathol. 2005, 167:489-503.
19. Bai H., Teng Y., Wong L., Jhanji V., Pang C.P., Yam G.H. Proliferative and migratory aptitude in pterygium. Histochem. Cell Biol. 2010, 134:527-535.
20. Clement A., Steele M.P., Brody J.S., Riedel N. SV40T-immortalized lung alveolar epithelial cells display post-transcriptional regulation of proliferation-related genes. Exp. Cell Res. 1991, 196:198-205.
21. Landry J.J., Pyl P.T., Rausch T., Zichner T., Tekkedil M.M., Stutz A.M., Jauch A., Aiyar R.S., Pau G., Delhomme N., Gagneur J., Korbel J.O., Huber W., Steinmetz L.M. The Genomic and Transcriptomic Landscape of a HeLa Cell Line. G3 (Bethesda) 2013, 8:1213-1224.
22. Delpino M.V., Fossati C.A., Baldi P.C. Proinflammatory response of human osteoblastic cell lines and osteoblast-monocyte interaction upon infection with Brucella spp. Infect. Immun. 2009, 77:984-995.
23. Alvarez M.B., Childress P., Philip B.K., Gerard-O'Riley R., Hanlon M., Herbert B.S., Robling A.G., Pavalko F.M., Bidwell J.P. Immortalization and characterization of osteoblast cell lines generated from wild-type and Nmp4-null mouse bone marrow stromal cells using murine telomerase reverse transcriptase (mTERT). J. Cell. Physiol. 2012, 227:1873-1882.
24. Naviaux R.K., Costanzi E., Haas M., Verma I.M. The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. J. Virol. 1996, 70:5701-5705.
25. Voorhoeve P.M., le Sage C., Schrier M., Gillis A.J., Stoop H., Nagel R., Liu Y.P., van Duijse J., Drost J., Griekspoor A., Zlotorynski E., Yabuta N., De Vita G., Nojima H., Looijenga L.H., Agami R. A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Cell 2006, 124:1169-1181.
26. Voorhoeve P.M., Agami R. The tumor-suppressive functions of the human INK4A locus. Cancer Cell 2003, 4:311-319.
27. Di Girolamo N., Tedla N., Kumar R.K., McCluskey P., Lloyd A., Coroneo M.T., Wakefield D. Culture and characterisation of epithelial cells from human pterygia. Br. J. Ophthalmol. 1999, 83:1077-1082.
28. Ho V., Yeo S.Y., Kunasegaran K., De Silva D., Tarulli G.A., Voorhoeve P.M., Pietersen A.M. Expression analysis of rare cellular subsets: direct RT-PCR on limited cell numbers obtained by FACS or soft agar assays. BioTechniques 2013, 54:208-212.
29. Voorhoeve P.M., Agami R. Unraveling human tumor suppressor pathways: a tale of the INK4A locus. Cell Cycle 2004, 3:616-620.
30. Counter C.M., Hahn W.C., Wei W., Caddle S.D., Beijersbergen R.L., Lansdorp P.M., Sedivy J.M., Weinberg R.A. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc. Natl. Acad. Sci. USA 1998, 95:14723-14728.
31. Kubota K., Furuse M., Sasaki H., Sonoda N., Fujita K., Nagafuchi A., Tsukita S. Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions. Curr. Biol. 1999, 9:1035-1038.
32. Dhawan P., Singh A.B., Deane N.G., No Y., Shiou S.R., Schmidt C., Neff J., Washington M.K., Beauchamp R.D. Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer. J. Clin. Invest. 2005, 115:1765-1776.
33. Leotlela P.D., Wade M.S., Duray P.H., Rhode M.J., Brown H.F., Rosenthal D.T., Dissanayake S.K., Earley R., Indig F.E., Nickoloff B.J., Taub D.D., Kallioniemi O.P., Meltzer P., Morin P.J., Weeraratna A.T. Claudin-1 overexpression in melanoma is regulated by PKC and contributes to melanoma cell motility. Oncogene 2007, 26:3846-3856.
34. Dos Reis P.P., Bharadwaj R.R., Machado J., Macmillan C., Pintilie M., Sukhai M.A., Perez-Ordonez B., Gullane P., Irish J., Kamel-Reid S. Claudin 1 overexpression increases invasion and is associated with aggressive histological features in oral squamous cell carcinoma. Cancer 2008, 113:3169-3180.
35. Collighan R.J., Griffin M. Transglutaminase 2 cross-linking of matrix proteins: biological significance and medical applications. Amino Acids 2009, 36:659-670.
36. Mehta K., Kumar A., Kim H.I. Transglutaminase 2: a multi-tasking protein in the complex circuitry of inflammation and cancer. Biochem. Pharmacol. 2010, 80:1921-1929.
37. Zhang R.Z., Zou Y., Pan T.C., Markova D., Fertala A., Hu Y., Squarzoni S., Reed U.C., Marie S.K., Bonnemann C.G., Chu M.L. Recessive COL6A2 C-globular missense mutations in Ullrich congenital muscular dystrophy: role of the C2a splice variant. J. Biol. Chem. 2010, 285:10005-10015.