Expression analysis of rare cellular subsets: Direct RT-PCR on limited cell numbers obtained by FACS or soft agar assays

BioTechniques

Volumn 54, Issue 4, 2013, Pages 208-211

  Ho, Victor ^a   Yeo, Shi Yun ^b   Kunasegaran, Kamini ^a   De Silva, Duvini ^a   Tarulli, Gerard A ^a   Voorhoeve, P Mathijs ^b,c   Pietersen, Alexandra M ^a,b,c  

^a DIVISION OF MEDICAL ONCOLOGY   (Singapore)

^b DUKE NUS MEDICAL SCHOOL   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

FACS; Gene expression profiling; RT QPCR; Soft agar assay

Indexed keywords

ALPHA6 INTEGRIN; CD24 ANTIGEN; CYTOKERATIN 8; GENOMIC DNA; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MESSENGER RNA; PROTEIN P53; SHORT HAIRPIN RNA;

ARTICLE; BREAST EPITHELIUM; CELL ISOLATION; FLUORESCENCE ACTIVATED CELL SORTING; FREEZE THAWING; GENE EXPRESSION; HUMAN; HUMAN CELL; LIMIT OF QUANTITATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AGAR; ANIMALS; BREAST; BREAST NEOPLASMS; CELL COUNT; CELLS, CULTURED; EPITHELIAL CELLS; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; HUMANS; MICE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

MAMMALIA;

EID: 84876738246     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114019     Document Type: Article

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