Synthesis of [18F]Fluorocholine ([18F]FCH)

Radiochemical Syntheses: Radiopharmaceuticals for Positron Emission Tomography

Volumn 1, Issue , 2012, Pages 61-68

  Kryza, David ^a  

^a UNIVERSITÉ LYON 1   (France)

Author keywords

QC procedures for 18F fluorocholine; Radiochemical identity, a Dionex 20 GP 50 gradient pump; Synthesis of 18F fluorocholine ( 18F FCH)

Indexed keywords

EID: 84886174621     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781118140345.ch7     Document Type: Chapter

References (31)

1. Hara T. 18F-Fluorocholine: a new oncologic PET tracer. J Nucl Med 2001;42(12):1815-1817.
2. Hara T, Kosaka N, Kondo T, Kishi H, Kobori O. Imaging of brain tumor, lung cancer, esophagus cancer, colon cancer, prostate cancer, and bladder cancer with [C-11]choline. J Nucl Med 1997; 38 Suppl:250P [abstract].
3. Hara T, Kosaka N, Kishi H. PET imaging of prostate cancer using carbon-11-choline. J Nucl Med 1998;39:990-995.
4. DeGrado TR, Baldwin SW, Wang S, Orr MD, Liao RP, et al. Synthesis and evaluation of (18)Flabeled choline analogs as oncologic PET 336 tracers. J Nucl Med 2001;42(12):1805-1814.
5. DeGrado TR, Coleman RE, Wang S, et al. Synthesis and evaluation of 18F-labeled choline as an oncologic tracer for positron emission tomography: initial findings in prostate cancer. Cancer Res 2001;61:110-117.
6. Kryza D, Tadino V, Filannino MA, Villeret G, Lemoucheux L. Nucl Med Biol 2008;35(2): 255-260.
7. GE Healthcare, USA.
8. From the website of GE Healthcare. Available at: http://www.gehealthcare.com/usen/fun_img/radiopharmacy/products/tracerlabmx.html
9. QMA-light Sep-Pak cartridges were purchased from Waters (part no. WAT023525) and conditioned with 10 mL ethanol, 10 ml water, 10 mL 0.5 M sodium bicarbonate, and a further 10 mL water before use.
10. Potassium carbonate was obtained from Sigma Aldrich (part no. 209619) and used as received. Sterile water (part no. 3120495) was purchased from Lavoisier and used as received.
11. Kryptofix-[2.2.2] was purchased from ABX (part no.800.1000) and used as received. Anhydrous acetonitrile (part no. 49,444-5) was purchased from Sigma-Aldrich and used as received.
12. Dibromomethane was obtained from Sigma Aldrich (part no. 239992) and used as received.
13. NReshearch, Switzerland.
14. Sep-Pak Plus silica cartridges were purchased from Waters (part no. WAT020520) and used as received.
15. Dimethylaminoethanol was obtained from Sigma Aldrich (part no. 391253) and used as received.
16. Sep-Pak Plus tC18 cartridges were purchased from Waters (part no. WAT036810) and used as received.
17. Accell™ plus CM Cartridges were purchased fromWaters (part no.WAT020550) and conditioned with 10 mL of 0.5M HCl.
18. Ethanol was purchased from Sigma Aldrich (part no. 32294) and used as received.
19. Sodium chloride 0.9% was purchased from Lavoisier (part no. 305-784-5) and used as received.
20. Dionex, Germany.
21. Bioscan, Inc., USA.
22. Analytical HPLC column: IonPac CS12A 4 × 250 mm column was purchased from Dionex (part no. P/N 44001) and guard column 4 × 50 mm was purchased from Dionex (part no. P/N 44002); mobile phase, 9 mM sulphuric acid; flow rate = 1.2 mL/min; analytical column was equilibrated with mobile phase for 30-60 min before use.
23. 199F]Fluorocholine was purchased from ABX (part no. 6130.0010) and used as received.
24. Varian, Inc., USA.
25. Analytical GC Column: Rtx® -200 was purchased from Restek (part no. 15024).
26. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH).
27. Merck chemicals, Germany (part no. 109584).
28. Mock BH, Winkle W, Vavrek MT. A color spot test for the detection of Kryptofix 2.2.2 in [18F] FDG preparations. Nucl Med Biol 1997;24:193-195.
29. Dose calibrator: Capintec CRC®-15R, Capintec, Inc., USA.
30. Charles River Laboratories, France.
31. Biomerieux Laboratories, France.