Modulating heterologous protein production in yeast: The applicability of truncated auxotrophic markers

Applied Microbiology and Biotechnology

Volumn 97, Issue 9, 2013, Pages 3939-3948

  Seresht, Ali Kazemi ^a,b   Nørgaard, Per ^a   Palmqvist, Eva Akke ^a   Andersen, Asser Sloth ^a   Olsson, Lisbeth ^b  

^a NOVO NORDISK A S   (Denmark)

^b CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

Author keywords

High cell density cultivation; Human insulin; LEU2d; Plasmid copy number; Promoter truncation; URA3d

Indexed keywords

BIOCHEMISTRY; DNA; GENES; INSULIN; PRODUCTIVITY; PROTEINS;

COPY NUMBER; HIGH-CELL-DENSITY CULTIVATIONS; HUMAN INSULINS; LEU2D; PROMOTER TRUNCATION; URA3D;

YEAST;

AMINO ACID; FUNGAL PROTEIN; HETEROLOGOUS PROTEIN; INSULIN DERIVATIVE; MARKER; PYRIMIDINE; UNCLASSIFIED DRUG; INSULIN; SACCHAROMYCES CEREVISIAE PROTEIN; URA3 PROTEIN, S CEREVISIAE;

BIOMARKER; CHEMOSTAT; ENZYME ACTIVITY; PROTEIN; RECOMBINATION; YEAST;

ARTICLE; AUXOTROPHY; BIOSYNTHESIS; CELL DENSITY; CHEMOSTAT; DNA EXTRACTION; ENZYME METABOLISM; EVOLUTIONARY ADAPTATION; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE DOSAGE; GENE EXPRESSION SYSTEM; GROWTH RATE; MARKER GENE; NONHUMAN; OPEN READING FRAME; PRECURSOR; PROMOTER REGION; RECOMBINANT GENE; SACCHAROMYCES CEREVISIAE; WILD TYPE; GENETICS; METABOLISM; PLASMID;

SACCHAROMYCES CEREVISIAE;

INSULIN; PLASMIDS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84885897049     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4263-1     Document Type: Article

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