Detection and quantification of schistosome DNA in freshwater snails using either fluorescent probes in real-time PCR or oligochromatographic dipstick assays targeting the ribosomal intergenic spacer

Acta Tropica

Volumn 128, Issue 2, 2013, Pages 241-249

  Kane, Richard A ^a   Stothard, J Russell ^a   Rollinson, David ^a   Leclipteux, Thierry ^b   Evraerts, Jonathan ^b   Standley, Claire J ^a   Allan, Fiona ^a   Betson, Martha ^a   Kaba, Rehana ^c   Mertens, Pascal ^b   Laurent, Thierry ^b  

^a DEPARTMENT OF LIFE SCIENCES   (United Kingdom)

^b R and D Department ^*  (Belgium)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Locked nucleic acid (LNA) probes; Molecular epidemiology; Oligochromatography; Real time PCR; Ribosomal intergenic spacer; Schistosomiasis

Indexed keywords

GENOMIC DNA; LOCKED NUCLEIC ACID; RIBOSOMAL INTERGENIC SPACER; SPACER DNA; UNCLASSIFIED DRUG;

CHROMATOGRAPHY; DETECTION METHOD; DNA; EPIDEMIOLOGY; FLUORESCENCE; POLYMERASE CHAIN REACTION; SCHISTOSOMIASIS; SNAIL;

AMPLICON; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DNA DETERMINATION; DNA PROBE; FLUORESCENCE; GENE AMPLIFICATION; NONHUMAN; OLIGOCHROMATOGRAPHY; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SCHISTOSOMA; SCHISTOSOMA HEMATOBIUM; SCHISTOSOMA MANSONI; SCHISTOSOMIASIS; SENSITIVITY AND SPECIFICITY; SNAIL;

BASOMMATOPHORA; GASTROPODA; SCHISTOSOMA; SCHISTOSOMA HAEMATOBIUM; SCHISTOSOMA MANSONI;

LOCKED NUCLEIC ACID (LNA) PROBES; MOLECULAR EPIDEMIOLOGY; OLIGOCHROMATOGRAPHY; REAL-TIME PCR; RIBOSOMAL INTERGENIC SPACER; SCHISTOSOMIASIS;

ANIMALS; CHROMATOGRAPHY; DNA, HELMINTH; FLUORESCENT DYES; FRESH WATER; MOLECULAR DIAGNOSTIC TECHNIQUES; PARASITE LOAD; REAL-TIME POLYMERASE CHAIN REACTION; SCHISTOSOMA HAEMATOBIUM; SCHISTOSOMA MANSONI; SNAILS;

EID: 84885473925     PISSN: 0001706X     EISSN: 18736254     Source Type: Journal    
DOI: 10.1016/j.actatropica.2011.10.019     Document Type: Article

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