Development of a high speed laser scanning confocal microscope with an acquisition rate up to 200 frames per second

Optics Express

Volumn 21, Issue 20, 2013, Pages 23611-23618

  Choi, S ^a   Kim, P ^b   Boutilier, R ^a   Kim, M Y ^a   Lee, Y J ^a   Lee, H ^a  

^a Kyungpook National University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFIERS (ELECTRONIC); ELECTROPHYSIOLOGY; FREQUENCY BANDS; SCANNING;

BI-DIRECTIONAL SCANNING; BIOLOGICAL PHENOMENA; FRAME ACQUISITION; FRAMES PER SECONDS; LASER SCANNING CONFOCAL MICROSCOPY; OPTICAL SECTIONING; PHOTO MULTIPLIER TUBE; SPATIAL RESOLUTION;

SPEED;

ANIMAL; ARTICLE; CONFOCAL MICROSCOPY; EQUIPMENT; HISTOLOGY; MOUSE; SKIN; TIME;

ANIMALS; MICE; MICROSCOPY, CONFOCAL; SKIN; TIME FACTORS;

EID: 84885459934     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.21.023611     Document Type: Article

References (10)

1. B. James, "Foundations of confocal scanned imaging in light microscopy," in Handbook of Biological Confocal Microscopy, Shinya Inoue, ed. (Plenum Press, 1990).
2. M. Minsky, "Microscopy apparatus," U.S. Patent 3013467 (1961).
3. C. M. Brown, R. B. Dalal, B. Hebert, M. A. Digman, A. R. Horwitz, and E. Gratton, "Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope," J. Microsc. 229(1), 78-91 (2008).
4. P. D. J. Moens, E. Gratton, and I. L. Salvemini, "Fluorescence Correlation Spectroscopy, Raster Image Correlation Spectroscopy, and Number and Brightness on a Commercial Confocal Laser Scanning Microscope with Analog Detectors (Nikon C1)," Microsc Res Techniq. 74, 377-388 (2011).
5. S. H. Choi, W. H. Kim, Y. J. Lee, W. J. Lee, J. D. Yang, J. W. Shim, J. W. Kim, and H. Lee, "Visualization of Epidermis and Dermal Cells in ex vivo Human Skin Using the Confocal and Two-photon Microscopy," j. Opt. Soc. Korea (South) 15, 61-67 (2011).
6. J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, "In vivo Cell Tracking With Video Rate Multimodality Laser Scanning Microscopy," IEEE J. Sel. Top. Quant 14, 10-18 (2008).
7. S. M. Choi, W. H. Kim, D. Côté, C. W. Park, and H. Lee, "Blood cell assisted in vivo Particle Image Velocimetry using the confocal laser scanning microscope," Opt. Express 19(5), 4357-4368 (2011).
8. T. Tanaami, S. Otsuki, N. Tomosada, Y. Kosugi, M. Shimizu, and H. Ishida, "High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks," Appl. Opt. 41, 4704-4708 (2002).
9. E. Wang, C. M. Babbey, and K. W. Dunn, "Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems," J. Microsc. 218(Pt 2), 148-159 (2005).
10. M. C. Adams, W. C. Salmon, S. L. Gupton, C. S. Cohan, T. Wittmann, N. Prigozhina, and C. M. Waterman-Storer, "A high-speed multispectral spinning-disk confocal microscope system for fluorescent speckle microscopy of living cells," Methods 29(1), 29-41 (2003).