Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae

Diagnostic Microbiology and Infectious Disease

Volumn 77, Issue 3, 2013, Pages 227-230

  Lee, Wonmok ^a,b   Chung, Hae Sun ^a,c   Lee, Yangsoon ^a,d   Yong, Dongeun ^a   Jeong, Seok Hoon ^a   Lee, Kyungwon ^a   Chong, Yunsop ^a  

^a Yonsei University College of Medicine   (South Korea)

^b Keimyung University School of Medicine   (South Korea)

^c Ewha Womans University School of Medicine   (South Korea)

^d National Health Insurance Corporation   (South Korea)

Author keywords

Acinetobacter spp; Carbapenemase; MALDI TOF MS; OXA; SIM 1

Indexed keywords

CARBAPENEMASE; IMP 6 PROTEIN; KPC 1 PROTEIN; NDM 1 PROTEIN; OXA 23 PROTEIN; OXA 51 PROTEIN; PROTEIN SIM 1; UNCLASSIFIED DRUG; VIM 2 PROTEIN;

ACINETOBACTER; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOASSAY; CONTROLLED STUDY; ENZYME ANALYSIS; HYDROLYSIS; INTERMETHOD COMPARISON; KLEBSIELLA PNEUMONIAE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

ACINETOBACTER SPP; CARBAPENEMASE; MALDI-TOF MS; OXA; SIM-1;

ACINETOBACTER; BACTERIOLOGICAL TECHNIQUES; BETA-LACTAMASES; HUMANS; KLEBSIELLA PNEUMONIAE; PSEUDOMONAS AERUGINOSA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

EID: 84885370695     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2013.07.005     Document Type: Article

References (24)

1. Bae I.K., Jeong S.H., Lee K. Carbapenem-resistant Acinetobacter baumannii. Ann Clin Microbiol 2012, 15:1-8.
2. Bernabeu S., Poirel L., Nordmann P. Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae. Diagn Microbiol Infect Dis 2012, 74:88-90.
3. Burckhardt I., Zimmermann S. Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours. J Clin Microbiol 2011, 49:3321-3324.
4. Carvalhaes C.G., Cayo R., Assis D.M., Martins E.R., Juliano L., Juliano M.A., Gales A.C. Detection of SPM-1-producing Pseudomonas aeruginosa and CHDL-producing Acinetobacter baumannii using liquid chromatography mass spectrometry (LCMS) and MALDI-TOF MS. J Clin Microbiol 2013, 51:287-290.
5. Carvalhaes C.G., Picao R.C., Nicoletti A.G., Xavier D.E., Gales A.C. Cloverleaf test (modified Hodge test) for detecting carbapenemase production in Klebsiella pneumoniae: be aware of false positive results. J Antimicrob Chemother 2010, 65:249-251.
6. Castanheira M., Deshpande L.M., Mathai D., Bell J.M., Jones R.N., Mendes R.E. Early dissemination of NDM-1- and OXA-181-producing Enterobacteriaceae in Indian hospitals: report from the SENTRY antimicrobial surveillance program, 2006-2007. Antimicrob Agents Chemother 2011, 55:1274-1278.
7. OXA-51-like gene with an upstream ISAba1 in carbapenem-resistant Acinetobacter baumannii isolates in Taiwan. Antimicrob Agents Chemother 2010, 54:4575-4581.
8. Performance standards for antimicrobial susceptibility testing. 22nd Informational supplement (M100-S22) 2012, Clinical and Laboratory Standards Institute (CLSI), CLSI, Wayne, PA.
9. Dortet L., Poirel L., Nordmann P. Rapid detection of carbapenemase-producing Pseudomonas spp. J Clin Microbiol 2012, 50:3773-3776.
10. Dortet L., Poirel L., Nordmann P. Rapid identification of carbapenemase types using a biochemical test in Enterobacteriaceae and Pseudomonas. Antimicrob Agents Chemother 2012, 56:6437-6440.
11. Girlich D., Poirel L., Nordmann P. Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae. J Clin Microbiol 2012, 50:477-479.
12. Kempf M., Bakour S., Flaudrops C., Berrazeg M., Brunel J.M., Drissi M., et al. Rapid detection of carbapenem resistance in Acinetobacter baumannii using matrix-assisted laser desorption ionization-time of flight mass spectrometry. PloS One 2012, 7:e31676.
13. Kim M., Kwon M.J., Chung H.S., Lee Y., Yong D., Jeong S.H., et al. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of aerobic bacteria in a clinical microbiology laboratory. Ann Clin Microbiol 2012, 15:60-66.
14. SIM-1-carrying Acinetobacter spp. from 2003 to 2008 in the hospital where it was initially discovered. Microb Drug Resist 2013, 19:37-41.
15. Lee K., Kim C.K., Yong D., Jeong S.H., Yum J.H., Seo Y.H., et al. Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. J Microbiol Methods 2010, 83:149-152.
16. Lee K., Kim M.N., Choi T.Y., Cho S.E., Lee S., Whang D.H., et al. Wide dissemination of OXA-type carbapenemases in clinical Acinetobacter spp. isolates from South Korea. Int J Antimicrob Agents 2009, 33:520-524.
17. SIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob Agents Chemother 2005, 49:4485-4491.
18. Lee Y., Lee J., Jeong S.H., Lee J., Bae I.K., Lee K. Carbapenem-non-susceptible Acinetobacter baumannii of sequence type 92 or its single-locus variants with a G428T substitution in zone 2 of the rpoB gene. J Antimicrob Chemother 2011, 66:66-72.
19. Nordmann P., Poirel L. Strategies for identification of carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother 2012, 68:487-489.
20. Nordmann P., Poirel L., Dortet L. Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 2012, 18:1503-1507.
21. Roh K.H., Kim S., Kim C.K., Yum J.H., Kim M.S., Yong D., et al. New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea. Diagn Microbiol Infect Dis 2010, 66:343-348.
22. Sparbier K., Schubert S., Weller U., Boogen C., Kostrzewa M. Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against β-lactam antibiotics. J Clin Microbiol 2012, 50:927-937.
23. Villegas M.V., Lolans K., Correa A., Suarez C.J., Lopez J.A., Vallejo M., et al. First detection of the plasmid-mediated class A carbapenemase KPC-2 in clinical isolates of Klebsiella pneumoniae from South America. Antimicrob Agents Chemother 2006, 50:2880-2882.
24. Yong D., Park R., Yum J.H., Lee K., Choi E.C., Chong Y. Further modification of the Hodge test to screen AmpC beta-lactamase (CMY-1)-producing strains of Escherichia coli and Klebsiella pneumoniae. J Microbiol Methods 2002, 51:407-410.