Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus

Toxicon

Volumn 76, Issue , 2013, Pages 132-142

  Bechlars, Silke ^a   Wüstenhagen, Doreen A ^b   Drägert, Katja ^a,c   Dieckmann, Ralf ^a   Strauch, Eckhard ^a   Kubick, Stefan ^b  

^a FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

^b FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^c UNIVERSITY HOSPITAL ZURICH   (Switzerland)

Author keywords

Cell free protein synthesis; Invitro translation; Thermostable direct hemolysin (TDH); Vibrio parahaemolyticus

Indexed keywords

GENOMIC DNA; HEMOLYSIN; THERMOSTABLE DIRECT HEMOLYSIN; UNCLASSIFIED DRUG;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; GENE AMPLIFICATION; HEMOLYSIS; ISOLATION AND PURIFICATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MULTICENTER STUDY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; REGULATORY SEQUENCE; VIBRIO PARAHAEMOLYTICUS; WESTERN BLOTTING;

EUKARYOTA; PROKARYOTA; VIBRIO PARAHAEMOLYTICUS;

CELL-FREE PROTEIN SYNTHESIS; IN VITRO TRANSLATION; THERMOSTABLE DIRECT HEMOLYSIN (TDH); VIBRIO PARAHAEMOLYTICUS;

BACTERIAL TOXINS; CELL-FREE SYSTEM; CLONING, MOLECULAR; ESCHERICHIA COLI K12; HEMOLYSIN PROTEINS; HEMOLYSIS; POLYMERASE CHAIN REACTION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; VIBRIO PARAHAEMOLYTICUS; VIRULENCE FACTORS;

EID: 84885360196     PISSN: 00410101     EISSN: 18793150     Source Type: Journal    
DOI: 10.1016/j.toxicon.2013.09.012     Document Type: Article

References (33)

1. Anonymous Risk Assessment of Vibrio parahaemolyticus in Seafood. World Health Organization - Food and Agriculture Organization of the United Nations 2011, ISBN 978-92-5-106874-8. http://www.fao.org/docrep/014/i2225e/i2225e00.pdf.
2. Baba K., Yamasaki S., Nishibuchi M., Takeda Y. Examination by site-directed mutagenesis of the amino acid residues of the thermostable direct hemolysin of Vibrio parahaemolyticus required for its hemolytic activity. Microb. Pathog. 1992, 12:279-287.
3. Broedel A.K., Raymond J.A., Duman J.G., Bier F.F., Kubick S. Functional evaluation of candidate ice structuring proteins using cell-free expression systems. J. Biotechnol. 2013, 163:301-310.
4. Carlson E.D., Gan R., Hodgman C.E., Jewett M.C. Cell-free protein synthesis: applications come of age. Biotechnol. Adv. 2012, 30:1185-1194.
5. Fabbri A., Falzano L., Frank C., Donelli G., Matarrese P., Raimondi F., Fasano A., Fiorentini C. Vibrio parahaemolyticus thermostable direct hemolysin modulates cytoskeletal organization and calcium homeostasis in intestinal cultured cells. Infect. Immun. 1999, 67:1139-1148.
6. Fuenzalida L., Armijo L., Zabala B., Hernandez C., Rioseco M.L., Riquelme C., Espejo R.T. Vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of Chile. Int. J. Food Microbiol. 2007, 117:270-275.
7. Fukui T., Shiraki K., Hamada D., Hara K., Miyata T., Fujiwara S., Mayanagi K., Yanagihara K., Iida T., Fukusaki E., Imanaka T., Honda T., Yanagihara I. Thermostable direct hemolysin of Vibrio parahaemolyticus is a bacterial reversible amyloid toxin. Biochemistry 2005, 44:9825-9832.
8. Hamada D., Higurashi T., Mayanagi K., Miyata T., Fukui T., Iida T., Honda T., Yanagihara I. Tetrameric structure of thermostable direct hemolysin from Vibrio parahaemolyticus revealed by ultracentrifugation, small-angle x-ray scattering and electron microscopy. J. Mol. Biol. 2007, 365:187-195.
9. Honda T., Ni Y.X., Miwatani T. Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin. Infect Immun. 1988, 56:961-965.
10. Iida T., Yamamoto K. Cloning and expression of two genes encoding highly homologous hemolysis from a Kanagawa-phenomenon-positive Vibrio parahaemolyticus T4750 strain. Gene 1990, 93:9-15.
11. Kim D.M., Kigawa T., Choi C.Y., Yokoyama S. A highly efficient cell-free, protein synthesis system from Escherichia coli. Eur. J. Biochem. 1996, 239:881-886.
12. Kim D.M., Swartz J.R. Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts of Escherichia coli. Biotechnol. Bioeng. 2004, 85:122-129.
13. Merk H., Meschkat D., Stiege W. Expression-PCR: from gene pools to purified proteins within 1 day. Cell-free Protein Expression 2003, 15-23. Springer, Berlin Heidelberg New York. J.R. Swartz (Ed.).
14. Martinez-Urtaza J., Simental L., Velasco D., DePaola A., Ishibashi M., Nakaguchi Y., Nishibuchi M., Carrera-Flores D., Rey-Alvarez C., Pousa A. Pandemic Vibrio parahaemolyticus O3:K6, Europe. Emerg. Infect Dis. 2005, 11:1319-1320.
15. Nair G.B., Ramamurthy T., Bhattacharya S.K., Dutta B., Takeda Y., Sack D.A. Global dissemination of Vibrio parahaemolyticus serotype O3:K6 and its serovariants. Clin. Microbiol. Rev. 2007, 20:39-48.
16. Nishibuchi M., Kaper J.B. Nucleotide sequence of the thermostable direct hemolysin gene of Vibrio parahaemolyticus. J. Bacteriol. 1985, 162:558-564.
17. Nishibuchi M., Kaper J.B. Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect Immun. 1995, 63:2093-2099.
18. Nishibuchi M., Kumagai K., Kaper J.B. Contribution of the tdh 1 gene of Kanagawa phenomenon-positive Vibrio parahaemolyticus to production of extracellular thermostable direct hemolysin. Microb. Pathog. 1991, 11:453-460.
19. Nishibuchi M., Shirai M., Yamasaki S., Terai A., Baba K., Kumagai K., Takeda Y. Dynamics of the thermostable direct hemolysin gene (tdh gene) of Vibrio species. Jpn J. Med. Sci. Biol. 1989, 42:195-196.
20. Okuda J., Nishibuchi M. Manifestation of the Kanagawa phenomenon, the virulence-associated phenotype, of Vibrio parahaemolyticus depends on a particular single base change in the promoter of the thermostable direct haemolysin gene. Mol. Microbiol. 1998, 30:499-511.
21. Orth J.H.C., Schorch B., Boundry S., Ffrench-Constant R., Kubick S., Aktories K. Cell-free synthesis and characterization of a novel cytotoxic pierisin-like protein from the cabbage butterfly Pieris rapae. Toxicon 2011, 57(Issue 2):199-207.
22. Ottaviani D., Leoni F., Rocchegiani E., Santarelli S., Canonico C., Masini L., Ditrani V., Carraturo A. First clinical report of pandemic Vibrio parahaemolyticus O3:K6 infection in Italy. J. Clin. Microbiol. 2008, 46:2144-2145.
23. Powell A., Baker-Austin C., Wagley S., Bayley A., Hartnell R. Isolation of Pandemic Vibrio parahaemolyticus from UK Water and Shellfish Produce. Microb. Ecol. 2013, 64:924-927.
24. Quilici M.L., Robert-Pillot A., Picart J., Fournier J.M. Pandemic Vibrio parahaemolyticus O3:K6 spread, France. Emerg. Infect Dis. 2005, 11:1148-1149.
25. Sambrook J., Russell D.W. Molecular Cloning: a Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. third ed.
26. Tang G.Q., Iida T., Yamamoto K., Honda T. A mutant toxin of Vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes. Infect. Immun. 1994, 62:3299-3304.
27. Tang G., Iida T., Yamamoto K., Honda T. Analysis of functional domains of Vibrio parahaemolyticus thermostable direct hemolysin using monoclonal antibodies. FEMS Microbiol. Lett. 1997, 150:289-296.
28. Taniguchi H., Ohta H., Ogawa M., Mizuguchi Y. Cloning and expression in Escherichia coli of Vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes. J. Bacteriol. 1985, 162:510-515.
29. Tsunasawa S., Sugihara A., Masaki T., Sakiyama F., Takeda Y., Miwatani T., Narita K. Amino acid sequence of thermostable direct hemolysin produced by Vibrio parahaemolyticus. J. Biochem. 1987, 101:111-121.
30. Yanagihara I., Nakahira K., Yamane T., Kaieda S., Mayanagi K., Hamada D., Fukui T., Ohnishi K., Kajiyama S., Shimizu T., Sato M., Ikegami T., Ikeguchi M., Honda T., Hashimoto H. Structure and functional characterization of Vibrio parahaemolyticus thermostable direct hemolysin. J. Biol. Chem. 2010, 285:16267-16274.
31. Yoh M. Characterization of thermostable direct hemolysins encoded by four representative tdh genes of Vibrio parahaemolyticus. Microb. Pathog. 1991, 10:165-172.
32. Yoh M., Tang G., Iida T., Morinaga N., Noda M., Honda T. Phosphorylation of a 25kDa Protein is induced by thermostable direct hemolysin of Vibrio parahaemolyticus. Int. J. Biochem. Cell. Bid. 1996, 28(12):1365-1369.
33. Zhao Y., Tang X., Zhan W. Cloning, expressing, and hemolysis of tdh, trh and tlh genes of Vibrio parahaemolyticus. J. Ocean Univ. China 2011, 10:275-279.