Generation of T cell receptor-retrogenic mice: Improved retroviral-mediated stem cell gene transfer

Nature Protocols

Volumn 8, Issue 10, 2013, Pages 1837-1840

  Bettini, Matthew L ^a   Bettini, Maria ^a   Nakayama, Maki ^b   Guy, Clifford S ^a   Vignali, Dario A A ^a  

^a ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

^b UNIVERSITY OF COLORADO DENVER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; FLUOROURACIL; INTERLEUKIN 3; INTERLEUKIN 6; PHOSPHATE BUFFERED SALINE; RETROVIRUS VECTOR; STEM CELL FACTOR;

APOPTOSIS; ARTICLE; BONE MARROW CELL; BONE MARROW TRANSPLANTATION; CELL FUNCTION; CLINICAL PROTOCOL; EXPERIMENTAL DESIGN; INCUBATION TEMPERATURE; INCUBATION TIME; MOUSE; NONHUMAN; PRIORITY JOURNAL; STEM CELL; SUPERNATANT; T CELL RECEPTOR RETROGENIC MOUSE; THAWING; TISSUE CULTURE; TRANSGENIC MOUSE; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; BONE MARROW TRANSPLANTATION; GENE TRANSFER TECHNIQUES; MICE; RECEPTORS, ANTIGEN, T-CELL; RETROVIRIDAE; TIME FACTORS;

EID: 84885017111     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.111     Document Type: Article

References (4)

1. Bettini, M.L., Bettini, M. & Vignali, D.A. T-cell receptor retrogenic mice: A rapid, flexible alternative to T-cell receptor transgenic mice. Immunology 136, 265-272 (2012
2. Holst, J. et al. Generation of T-cell receptor retrogenic mice. Nat. Protoc. 1, 406-417 (2006
3. Guy, C.S. et al. Distinct TCR signaling pathways drive proliferation and cytokine production in T cells. Nat. Immunol. 14, 262-270 (2013
4. Nakayama, M. et al. Germline TRAV5D-4 T-cell receptor sequence targets a primary insulin peptide of NOD mice. Diabetes 61, 857-865 (2012