Characterization of human, mouse, and rat cultures of enteric glial cells and their effect on intestinal epithelial cells

Neurogastroenterology and Motility

Volumn 25, Issue 11, 2013, Pages e755-e764

  Soret, R ^a,b,c,d   Coquenlorge, S ^a,b,c,d   Cossais, F ^a,b,c,d   Meurette, G ^a,b,c   Rolli Derkinderen, M ^a,b,c,d   Neunlist, M ^a,b,c,d  

^a INSERM   (France)

^b UNIVERSITÉ DE NANTES   (France)

^c HÔTEL DIEU   (France)

^d Ctr de Rech en Nutrition Humaine   (France)

Author keywords

Calcium signaling; Enteric glial cells; Enteric nervous system; Intestinal epithelial cells

Indexed keywords

ADENOSINE TRIPHOSPHATE; GLIAL FIBRILLARY ACIDIC PROTEIN; PROTEIN S100B; SMOOTH MUSCLE ACTIN; TRANSCRIPTION FACTOR SOX10;

ADULT; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CALCIUM CELL LEVEL; CELL ISOLATION; CELL PROLIFERATION; CELL SPREADING; FEMALE; GLIA CELL; GLIAL CELL CULTURE; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; INTESTINE EPITHELIUM CELL; MALE; MICRODISSECTION; MOUSE; MYENTERIC PLEXUS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; TISSUE CHARACTERIZATION;

CALCIUM SIGNALING; ENTERIC GLIAL CELLS; ENTERIC NERVOUS SYSTEM; INTESTINAL EPITHELIAL CELLS;

ADENOSINE TRIPHOSPHATE; ADULT; AGED; AGED, 80 AND OVER; ANIMALS; CALCIUM; CELL CULTURE TECHNIQUES; EPITHELIAL CELLS; FEMALE; HUMANS; INTESTINAL MUCOSA; MALE; MICE; MICE, INBRED C57BL; MIDDLE AGED; MYENTERIC PLEXUS; NEUROGLIA; RATS; RATS, SPRAGUE-DAWLEY; YOUNG ADULT;

EID: 84884988853     PISSN: 13501925     EISSN: 13652982     Source Type: Journal    
DOI: 10.1111/nmo.12200     Document Type: Article

References (33)

1. Schemann M, Neunlist M. The human enteric nervous system. Neurogastroenterol Motil 2004; 16(Suppl. 1): 55-9.
2. Abdo H, Derkinderen P, Gomes P et al. Enteric glial cells protect neurons from oxidative stress in part via reduced glutathione. Faseb J 2011; 24: 1082-94.
3. Cirillo C, Sarnelli G, Turco F et al. Proinflammatory stimuli activates human-derived enteroglial cells and induces autocrine nitric oxide production. Neurogastroenterol Motil 2011; 23: e372-82.
4. Neunlist M, Aubert P, Bonnaud S et al. Enteric glia inhibit intestinal epithelial cell proliferation partly through a TGF-beta1-dependent pathway. Am J Physiol Gastrointest Liver Physiol 2007; 292: G231-41.
5. Neunlist M, Van Landeghem L, Bourreille A, Savidge T. Neuro-glial crosstalk in inflammatory bowel disease. J Intern Med 2008; 263: 577-83.
6. Savidge TC, Newman P, Pothoulakis C et al. Enteric glia regulate intestinal barrier function and inflammation via release of S-nitrosoglutathione. Gastroenterology 2007; 132: 1344-58.
7. Savidge TC, Sofroniew MV, Neunlist M. Starring roles for astroglia in barrier pathologies of gut and brain. Lab Invest 2007; 87: 731-6.
8. Gulbransen BD, Sharkey KA. Novel functional roles for enteric glia in the gastrointestinal tract. Nat Rev Gastroenterol Hepatol 2012; 9: 625-32.
9. Neunlist M, Van Landeghem L, Mahe MM, Derkinderen P, des Varannes SB, Rolli-Derkinderen M. The digestive neuronal-glial-epithelial unit: a new actor in gut health and disease. Nat Rev Gastroenterol Hepatol. 2013; 10: 90-100.
10. Aube AC, Cabarrocas J, Bauer J et al. Changes in enteric neurone phenotype and intestinal functions in a transgenic mouse model of enteric glia disruption. Gut 2006; 55: 630-7.
11. Bush TG, Savidge TC, Freeman TC et al. Fulminant jejuno-ileitis following ablation of enteric glia in adult transgenic mice. Cell 1998; 93: 189-201.
12. Cornet A, Savidge TC, Cabarrocas J et al. Enterocolitis induced by autoimmune targeting of enteric glial cells: a possible mechanism in Crohn's disease? Proc Natl Acad Sci USA 2001; 98: 13306-11.
13. Nasser Y, Fernandez E, Keenan CM et al. Role of enteric glia in intestinal physiology: effects of the gliotoxin fluorocitrate on motor and secretory function. Am J Physiol Gastrointest Liver Physiol 2006; 291: G912-27.
14. Bannerman PG, Mirsky R, Jessen KR. Establishment and properties of separate cultures of enteric neurons and enteric glia. Brain Res 1988; 440: 99-108.
15. Ruhl A, Trotter J, Stremmel W. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat. Neurogastroenterol Motil 2001; 13: 95-106.
16. Bernstein CN, Vidrich A. Isolation, identification, and culture of normal mouse colonic glia. Glia 1994; 12: 108-16.
17. Van Landeghem L, Chevalier J, Mahe MM et al. Enteric glia promote intestinal mucosal healing via activation of focal adhesion kinase and release of proEGF. Am J Physiol Gastrointest Liver Physiol 2011; 300: G976-87.
18. Van Landeghem L, Mahe MM, Teusan R et al. Regulation of intestinal epithelial cells transcriptome by enteric glial cells: impact on intestinal epithelial barrier functions. BMC Genomics 2009; 10: 507.
19. Turco F, Sarnelli G, Cirillo C et al. Enteroglial-derived S100B protein integrates bacteria-induced Toll-like receptor signalling in human enteric glial cells. Gut 2013 (Online First).
20. Cirillo C, Sarnelli G, Esposito G et al. Increased mucosal nitric oxide production in ulcerative colitis is mediated in part by the enteroglial-derived S100B protein. Neurogastroenterol Motil 2009; 21: 1209-e1112.
21. Villanacci V, Bassotti G, Nascimbeni R et al. Enteric nervous system abnormalities in inflammatory bowel diseases. Neurogastroenterol Motil 2008; 20: 1009-16.
22. Gomes P, Chevalier J, Boesmans W et al. ATP-dependent paracrine communication between enteric neurons and glia in a primary cell culture derived from embryonic mice. Neurogastroenterol Motil 2009; 21: 870-e862.
23. Gulbransen BD, Bains JS, Sharkey KA. Enteric glia are targets of the sympathetic innervation of the myenteric plexus in the guinea pig distal colon. J Neurosci 2010; 30: 6801-9.
24. Gulbransen BD, Sharkey KA. Purinergic neuron-to-glia signaling in the enteric nervous system. Gastroenterology 2009; 136: 1349-58.
25. Ruhl A, Franzke S, Collins SM, Stremmel W. Interleukin-6 expression and regulation in rat enteric glial cells. Am J Physiol Gastrointest Liver Physiol 2001; 280: G1163-71.
26. Boesmans W, Cirillo C, Van den Abbeel V et al. Neurotransmitters involved in fast excitatory neurotransmission directly activate enteric glial cells. Neurogastroenterol Motil 2013; 25: e151-60.
27. Koliatsos VE, Price DL. Axotomy as an experimental model of neuronal injury and cell death. Brain Pathol 1996; 6: 447-65.
28. Guidry C. Isolation and characterization of porcine Muller cells. Myofibroblastic dedifferentiation in culture. Invest Ophthalmol Vis Sci 1996; 37: 740-52.
29. Hoff S, Zeller F, von Weyhern CW et al. Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8/9/10 antibody. J Comp Neurol 2008; 509: 356-71.
30. von Boyen G, Steinkamp M. The role of enteric glia in gut inflammation. Neuron Glia Biol 2010; 6: 231-6.
31. Boesmans W, Vanden Berghe P, Pachnis V. Glial heterogeneity in the enteric nervous system. Neurogastroenterol Motil 2012; 24: 36.
32. Galligan JJ, Bertrand PP. ATP mediates fast synaptic potentials in enteric neurons. J Neurosci 1994; 14: 7563-71.
33. Monro RL, Bornstein JC, Bertrand PP. Synaptic transmission from the submucosal plexus to the myenteric plexus in Guinea-pig ileum. Neurogastroenterol Motil 2008; 20: 1165-73.