Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat

Neurogastroenterology and Motility

Volumn 13, Issue 1, 2001, Pages 95-106

  Ruhl A ^a,b   Trotter, J ^a   Stremmel, W ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

Cell lines; Enteric glia; Rat; Tissue culture

Indexed keywords

ALPHA ACTIN; COMPLEMENT COMPONENT C3 RECEPTOR; DESMIN; GLIAL FIBRILLARY ACIDIC PROTEIN; MESSENGER RNA; MUSCLE PROTEIN; PROTEIN C FOS; PROTEIN S 100; VIMENTIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL LINEAGE; CELL TRANSFORMATION; CELL TYPE; CONTROLLED STUDY; CYTOLYSIS; DOT HYBRIDIZATION; ENTERITIS; GENE TRANSFER; GLIA CELL; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; INTESTINE CELL; INTESTINE INNERVATION; MALE; MYENTERIC PLEXUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RECEPTOR UPREGULATION; SMOOTH MUSCLE FIBER;

ANIMALS; CELL LINE; CELL SEPARATION; ENTERIC NERVOUS SYSTEM; GENES, FOS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MYENTERIC PLEXUS; NERVE TISSUE PROTEINS; NEUROGLIA; RATS; RETROVIRIDAE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0035117178     PISSN: 13501925     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2982.2001.00246.x     Document Type: Article

References (58)

1. Auerbach's plexus of mammals and man: Electron microscopic identification of three different types of neuronal processes in myenteric ganglia of the large intestine from rhesus monkeys, guinea-pigs and man
2. The ultrastructure of Auerbach's plexus in the guinea-pig. II. Non-neuronal elements
3. The ultrastructure of Auerbach's plexus in the guinea-pig. I. Neuronal elements
4. Ultrastructure of the nerve plexuses of the mammalian intestine: The enteric glial cells
5. Enteric glia
6. Glial cells in the enteric nervous system contain glial fibrillary acidic protein
7. Evidence for the presence of S-100 protein in the glial component of the human enteric nervous system
8. Astrocyte-like glia in the peripheral nervous system: An immunohistochemical study of enteric glia
9. A surface protein expressed by avian myelinating and nonmyelinating Schwann cells but not by satellite or enteric glial cells
10. Molecular profile of reactive astrocytes - Implications for their role in neurologic disease
11. Major histocompatibility class II expression on the small intestinal nervous system in Crohn's disease
12. Enteroglial cells (EGC) are an integral part of the neuroimmune axis in the enteric nervous system (ENS)
13. Fulminant jejuno-ileitis following ablation of enteric glia in adult transgenic mice
14. Enteroglial cells from rat myenteric plexus express interleukin-1β and interleukin-6 mRNA
15. Immortalization of neural cells via retrovirus-mediated oncogene transduction
16. The use of cell lines in neurobiology
17. Establishment and characterization of multipotent neural cell lines using retrovirus vector-mediated oncogene transfer
18. Lines of murine oligodendroglial precursor cells immortalized by an activated neu tyrosine kinase show distinct degrees of interaction with axons in vitro and in vivo
19. Schwann cell growth factors
20. Schwann cell precursors and their development
21. Studies on cultured rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerve
22. A chimeric EGF-R-neu proto-oncogene allows EGF to regulate neu tyrosine kinase and cell transformation
23. Effect of calcium ions on S-100, a protein of the nervous system
24. Purification of rat Schwann cells from cultures of peripheral nerve: An immunoselective method using surfaces coated with anti-immunoglobulin antibodies
25. A surface antigenic marker for rat Schwann cells
26. Vault immunofluorescence in the brain: New insights regarding the origin of microglia
27. Axotomy of the rat facial nerve leads to increased CR3 complement receptor expression by activated microglial cells
28. Immunocytochemical localization of CR3 complement receptors with OX-42 in amoeboid microglia in postnatal rats
29. Early changes of macrophage-like immunoreactivity in the rat inferior olive after intraperitoneal 3-acetylpyridine injection
30. Immunocytochemical and electron-microscopic characterization of macrophage/microglia cells and expression of class II major histocompatibility complex in the pineal gland of the rat
31. Persistence of fluorogold following degeneration of labeled motor neurons is due to phagocytosis by microglia and macrophages
32. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3
33. Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies
34. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
35. Inflammatory bowel disease: Etiology and pathogenesis
36. The enteric nervous system in tissue culture. I. Cell types and their interactions in explants of the myenteric and submucous plexuses from guinea pig, rabbit and rat
37. The enteric nervous system in tissue culture. II. Ultrastructural studies of cell types and their relationships
38. Analysis of enteric neurons, glia and their interactions using explant cultures of the myenteric plexus
39. Neurons and glial cells of the enteric nervous system: Studies in tissue culture
40. Human newborn and adult myenteric plexus grows in different patterns
41. Isolation, identification, and culture of normal mouse colonic glia
42. Characterization of normal human brain cultures. Evidence for the outgrowth of leptomeningeal cells
43. Leukocytes of the intestinal muscularis: Their phenotype and isolation
44. Regulation by EGF is maintained in an overexpressed chimeric EGFR/neu receptor tyrosine kinase
45. In vitro and in vivo characterisation of glial cells immortalised with a temperature sensitive SV40 T antigen-containing retrovirus
46. The immortalized astroglial cell line RC7 is a new model system for the study of nerve growth factor (NGF) regulation: Stimulation by interleukin-1 beta and transforming growth factor-beta 1 is additive and affected differently by dibutyryl cyclic AMP
47. Isolation and characterization of conditionally immortalized astrocyte cell lines derived from adult human spinal cord
48. Activation of an EGFR/neu chimeric receptor: Early intracellular signals and cell proliferation responses
49. Activation of the neu tyrosine kinase induces the fos/jun transcription factor complex, the glucose transporter and ornithine decarboxylase
50. Downregulation of the early genomic growth factor response in neu oncogene-transformed cells
51. Constitutively activated neu oncoprotein tyrosine kinase interferes with growth factor-induced signals for gene activation
52. Stimulus-transcription coupling in the nervous system: Involvement of the inducible protooncogenes fos and jun
53. The c-fos protein interacts with c-jun/AP-1 to stimulate transcription of AP-1 responsive genes
54. The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites
55. The fos gene as "master switch"
56. Common DNA binding site for Fos protein complexes and transcription factor AP-1
57. Immediate-early gene expression in the inferior mesenteric ganglion and colonic myenteric plexus of the guinea pig
58. HER2 cytoplasmic domain generates normal mitogenic and transforming signals in a chimeric receptor