An aptamer-based trypsin reactor for on-line protein digestion with electrospray ionization tandem mass spectrometry

Analytical Biochemistry

Volumn 441, Issue 2, 2013, Pages 123-132

  Xiao, Peng ^a,b   Lv, Xuefei ^a   Wang, Shanshan ^c   Iqbal, Javed ^a   Qing, Hong ^a   Li, Qin ^a   Deng, Yulin ^a  

^a BEIJING INSTITUTE OF TECHNOLOGY   (China)

^b NATIONAL INSTITUTE OF METROLOGY   (China)

^c Shandong Analysis and Test Center   (China)

Author keywords

Aptamer; Digestion; Mass spectrometry identification; Protein; Trypsin

Indexed keywords

DISSOCIATION; DNA; ENZYME IMMOBILIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEINS; SILICA; SLUDGE DIGESTION;

APTAMERS; ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRIES; IMMOBILIZED TRYPSIN REACTORS; IN-SOLUTION DIGESTIONS; PROTEIN IDENTIFICATION; SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENTS; TRYPSIN;

ELECTROSPRAY IONIZATION;

APTAMER; SINGLE STRANDED DNA; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL MODIFICATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DISSOCIATION CONSTANT; ELECTROSPRAY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; HYDROLYSIS; IONIC STRENGTH; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN RENATURATION; REACTION TIME; REACTOR; TANDEM MASS SPECTROMETRY;

APTAMER; DIGESTION; MASS SPECTROMETRY IDENTIFICATION; PROTEIN; TRYPSIN;

ANIMALS; APTAMERS, NUCLEOTIDE; CATTLE; CHICKENS; CHROMATOGRAPHY, LIQUID; DNA, SINGLE-STRANDED; ENZYMES, IMMOBILIZED; PROTEINS; PROTEOLYSIS; PROTEOMICS; SILICON DIOXIDE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84884962201     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.06.012     Document Type: Article

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