Two continuous coupled assays for ornithine-δ-aminotransferase

Analytical Biochemistry

Volumn 440, Issue 2, 2013, Pages 145-149

  Juncosa, Jose I ^a   Lee, Hyunbeom ^a   Silverman, Richard B ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Amplex Red; Continuous assay; Coupled assay; D1 Pyrroline 5 carboxylate reductase 1; L Glutamate oxidase; Ornithine d aminotransferase

Indexed keywords

CARBOXYLATION; MOLECULES; NUCLEOTIDES;

AMINOTRANSFERASE; AMPLEX RED; CONTINUOUS ASSAY; D1-PYRROLINE-5-CARBOXYLATE REDUCTASE 1; L-GLUTAMATE OXIDASE;

AMINO ACIDS;

GLUTAMIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; ORNITHINE; ORNITHINE OXOACID AMINOTRANSFERASE; PYRROLINE 5 CARBOXYLATE REDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; CATALYSIS; CONTINUOUS COUPLED ASSAY; ENZYME ASSAY; ENZYME METABOLISM; FLUORESCENCE; HUMAN; PRIORITY JOURNAL;

AMPLEX RED; CONTINUOUS ASSAY; COUPLED ASSAY; L-GLUTAMATE OXIDASE; ORNITHINE-Δ-AMINOTRANSFERASE; Δ(1)-PYRROLINE-5-CARBOXYLATE REDUCTASE 1;

BIOCATALYSIS; ENZYME ASSAYS; GLUTAMIC ACID; HUMANS; ORNITHINE; ORNITHINE-OXO-ACID TRANSAMINASE; PYRROLINE CARBOXYLATE REDUCTASES;

EID: 84884918424     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.05.025     Document Type: Article

References (13)

1. N. Seiler, Ornithine aminotransferase, a potential target for the treatment of hyperammonemias, Curr. Drug Targets 1 (2000) 119-153.
2. I. Sorri, M.G. Brigell, M. Malyusz, E. Mahlamaki, C. de Meynard, R. Kalviainen, Is reduced ornithine-d-aminotransferase activity the cause of vigabatrinassociated visual field defects?, Epilepsy Res 92 (2010) 48-53.
3. T. Ohura, E. Kominami, N. Katunuma, A new sensitive and convenient assay of ornithine aminotransferase, J. Nutr. Sci. Vitaminol. 29 (1983) 123-128.
4. H.J. Strecker, Purification and properties of rat liver ornithine d-transaminase, J. Biol. Chem. 240 (1965) 1225-1230.
5. H.R. Kim, H.W. Rho, J.W. Park, B.H. Park, J.S. Kim, M.W. Lee, Assay of ornithine aminotransferase with ninhydrin, Anal. Biochem. 223 (1994) 205-207. (Pubitemid 124007178)
6. Z. Meng, Z. Lou, Z. Liu, M. Li, X. Zhao, M. Bartlam, Z. Rao, Crystal structure of human pyrroline-5-carboxylate reductase, J. Mol. Biol. 359 (2006) 1364-1377. (Pubitemid 43903503)
7. I.M.A. da Rocha, V.A. Vitorello, J.S. Silva, S.L. Ferreira-Silva, R.A. Viegas, E.N. Silva, J.A.G. Silveira, Exogenous ornithine is an effective precursor and the dornithine amino transferase pathway contributes to proline accumulation under high N recycling in salt-stressed cashew leaves, J. Plant Physiol. 169 (2012) 41-49.
8. J. R. M. Lampinen, A. Perala, H. Oranen, R-R. Harinen, Microplate based pathlength correction method for photometric DNA quantification assay, Application Notes [online], 2012, http://www.thermoscientific.de/eThermo/CMA/ PDFs/Various/File-60220.pdf.
9. L. Rover Jr., J.C.B. Fernandes, G. de Oliveira Neto, L.T. Kubota, E. Katekawa, S.H.P. Serrano, Study of NADH stability using ultraviolet-visible spectrophotometric analysis and factorial design, Anal. Biochem. 260 (1998) 50-55. (Pubitemid 28285827)
10. K. Kiianitsa, J.A. Solinger, W.-D. Heyer, NADH-coupled microplate photometric assay for kinetic studies of ATP-hydrolyzing enzymes with low and high specific activities, Anal. Biochem. 321 (2003) 266-271. (Pubitemid 37162750)
11. T. Ohura, E. Kominami, K. Tada, N. Katunuma, Crystallization and properties of human-liver ornithine aminotransferase, J. Biochem. 92 (1982) 1785-1792. (Pubitemid 13067721)
12. J.I. Juncosa Jr., A.P. Groves, G. Xia, R.B. Silverman, Probing the steric requirements of the c-aminobutyric acid aminotransferase active site with fluorinated analogues of vigabatrin, Bioorg. Med. Chem. 21 (2013) 903-911.
13. J. Arima, M. Ashiuchi, K. Inagaki, H. Kusakabe, T. Yagi, L-Glutamate oxidase, U.S. patent 7,109,008, filed April 19, 2001, and issued September 19, 2006.