Isolation of mitochondria from mammalian tissues and cultured cells

Cell Biology, Four-Volume Set

Volumn 2, Issue , 2006, Pages 69-77

  Fernández Vizarra, Erika ^a   Fernández Silva, Patricio ^a   Enríquez, José A ^a  

^a UNIVERSITY OF ZARAGOZA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84884858773     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-012164730-8/50082-4     Document Type: Chapter

References (23)

1. Andreu A.L., Arbos M.A., Perez-Martos A., Lopez-Perez M.J., Asin J., Lopez N., Montoya J., Schwartz S. Reduced mitochondrial DNA transcription in senescent rat heart. Biochem. Biophys. Res. Commun. 1998, 252:577-581.
2. Baudhuin P. Isolation of rat liver peroxisomes. Methods Enzymol. 1974, 31:356-368.
3. Birch-Machin M.A., Turnbull D.M. Assaying mitochondrial respiratory complex activity in mitochondria isolated from human cells and tissues. Methods Cell Biol. 2001, 65:97-117.
4. Diez-Sanchez C., Ruiz-Pesini E., Lapena A.C., Montoya J., Perez-Martos A., Enriquez J.A., Lopez-Perez M.J. Mitochondrial DNA content of human spermatozoa. Biol. Reprod. 2003, 68:180-185.
5. Enríquez J.A., Attardi G. Analysis of Aminoacylation of Human Mitochondrial tRNAs. Methods Enzymol. 1996, 264:183-196.
6. Enríquez J.A., Fernández-Silva P., Garrido-Pérez N., López-Pérez M.J., Pérez-Martos A., Montoya J. Direct regulation of mitochondrial RNA synthesis by thyroid hormone. Mol. Cell Biol. 1999, 19:657-670.
7. Enriquez J.A., Lopez-Perez M.J., Montoya J. Saturation of the processing of newly synthesized rRNA in isolated brain mitochondria. FEBS Lett. 1991, 280:32-36.
8. Enriquez J.A., Perez-Martos A., Lopez-Perez M.J., Montoya J. In organello RNA synthesis system from mammalian liver and brain. Methods Enzymol. 1996, 264:50-57.
9. Enriquez J.A., Sanchez-Prieto J., Muino Blanco M.T., Hernandez-Yago J., Lopez-Perez M.J. Rat brain synaptosomes prepared by phase partition. J. Neurochem. 1990, 55:1841-1849.
10. Fernandez-Vizarra E., Lopez-Perez M.J., Enriquez J.A. Isolation of biogenetically compenent mitochondria from mammalian tissues and cultured cells. Methods 2002, 26:292-297.
11. Gaines G.L. In organello RNA synthesis system from HeLa cells. Methods Enzymol. 1996, 264:43-49.
12. Lopez-Perez M.J. Preparation of synaptosomes and mitochondria from mammalian brain. Methods Enzymol. 1994, 228:403-411.
13. McKee E.E., Grier B.L., Thompson G.S., McCourt J.D. Isolation and incubation conditions to study heart mitochondrial protein synthesis. Am. J. Physiol. 1990, 258:E492-E502.
14. Micol V., Fernandez-Silva P., Attardi G. Functional analysis of in vivo and in organello footprinting of HeLa cell mitochondrial DNA in relationship to ATP and ethidium bromide effects on transcription. J. Biol. Chem. 1997, 272:18896-18904.
15. Morré D.J. Isolation of Golgi apparatus. Methods Enzymol. 1971, 31:130-148.
16. Olafson R.W., Drummond G.I., Lee J.F. Studies on 2',3'-cyclic nucleotide-3'-phosphohydrolase from brain. Can. J. Biochem. 1969, 47:961-966.
17. Palloti F., Lenaz G. Isolation and subfractionation of mitochondria from animal cells and tissue culture lines. Methods Cell Biol. 2001, 65:1-35.
18. Rickwood D., Wilson M.T., Darley-Usmar V.M. Isolation and characteristics of intact mitochondria. Mitochondria: A Practical Approach 1987, 1-16. IRL Press, Oxford. V.M. Darley-Usmar, D. Rickwood, M.T. Wilson (Eds.).
19. Srere P.A. Citrate synthase. Methods Enzymol. 1969, 13:3-11.
20. Trouet A. Isolation of modified liver lysosomes. Methods Enzymol. 1974, 31:323-329.
21. Trounce I.A., Kim Y.L., Jun A.S., Wallace D.C. Assessment of mitochondrial oxidative phosphorylation in patient muscle biopsies, lymphoblasts, and transmitochondrial cell lines. Methods Enzymol. 1996, 264:484-509.
22. Wharton D.C., Tzagoloff A. Cytochrome oxidase from beef heart mitochondria. Methods Enzymol. 1967, 10:245-250.
23. Whittaker V.P. Thirty years of synaptosome research. J. Neurocytol. 1993, 22:735-742.