Downregulation of KPNA2 in non-small-cell lung cancer is associated with Oct4 expression

Journal of Translational Medicine

Volumn 11, Issue 1, 2013, Pages

  Li, Xiao Lei ^a,b   Jia, Lan Ling ^a   Shi, Mu Mu ^a,b   Li, Xin ^a,c   Li, Zhong Hua ^d   Li, Hui Feng ^e   Wang, En Hua ^a   Jia, Xin Shan ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

^b Daqing Branch of Haerbin Medical University   (China)

^c SHENYANG PHARMACEUTICAL UNIVERSITY   (China)

^d Daqing People's Hospital   (China)

^e Daqing Oilfield General Hospital   (China)

Author keywords

KPNA2; Non small cell lung cancer; Nucleocytoplasmic transport; Oct4

Indexed keywords

IMPORTIN ALPHA1; KARYOPHERIN; KPNA2; MESSENGER RNA; NUCLEOPROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CANCER CELL CULTURE; CANCER GROWTH; CANCER PROGNOSIS; CANCER STAGING; CANCER TISSUE; CELL NUCLEUS; CELL PROLIFERATION; CLINICAL FEATURE; CONTROLLED STUDY; DOWN REGULATION; FEMALE; GENE SILENCING; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; LUNG NON SMALL CELL CANCER; LUNG PARENCHYMA; MAJOR CLINICAL STUDY; MALE; OVERALL SURVIVAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE DIFFERENTIATION; WESTERN BLOTTING;

ALPHA KARYOPHERINS; CARCINOMA, NON-SMALL-CELL LUNG; CELL LINE, TUMOR; CELL PROLIFERATION; DOWN-REGULATION; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; KAPLAN-MEIER ESTIMATE; LUNG NEOPLASMS; MALE; MIDDLE AGED; OCTAMER TRANSCRIPTION FACTOR-3; PROTEIN BINDING;

EID: 84884754337     PISSN: None     EISSN: 14795876     Source Type: Journal    
DOI: 10.1186/1479-5876-11-232     Document Type: Article

References (34)

1. Takeda J, Seino S, Bell GI. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Res 1992, 20:4613-4620. 10.1093/nar/20.17.4613, 334192, 1408763.
2. Boiani M, Scholer HR. Regulatory networks in embryo-derived pluripotent stem cells. Nat Rev Mol Cell Biol 2005, 6:872-884. 10.1038/nrm1744, 16227977.
3. Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 2000, 24:372-376. 10.1038/74199, 10742100.
4. Chen YC, Hsu HS, Chen YW, Tsai TH, How CK, Wang CY, Hung SC, Chang YL, Tsai ML, Lee YY, Ku HH, Chiou SH. Oct-4 expression maintained cancer stem-like properties in lung cancer derived CD133-positive cells. PLoS One 2008, 3:e2637. 10.1371/journal.pone.0002637, 2440807, 18612434.
5. Huang PZ, Lu CL, Li BK, Hong J, Huang L, Wang L, Zhang Y, Yuan YF. OCT4 expression in hepatocellular carcinoma and its clinical significance. Ai Zheng 2010, 29:105-109.
6. Al-Marzoqee FY, Khoder G, Al-Awadhi H, John R, Beg A, Vincze A, Branicki F, Karam SM. Upregulation and inhibition of the nuclear translocation of Oct4 during multistep gastric carcinogenesis. Int J Oncology 2012, 41:1733-1743.
7. Ge N, Lin HX, Xiao XS, Guo L, Xu HM, Wang X, Jin T, Cai XY, Liang Y, Hu WH, Kang TB. Prognostic significance of Oct4 and Sox2 expression in hypopharyngeal squamous cell carcinoma. J Transl Med 2010, 8:94. 10.1186/1479-5876-8-94, 2958910, 20937145.
8. Ponti D, Costa A, Zaffaroni N, Pratesi G, Petrangolini G, Coradini D, Pilotti S, Pierotti MA, Daidone MG. Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Cancer Res 2005, 65:5506-5511. 10.1158/0008-5472.CAN-05-0626, 15994920.
9. Wang X, Dai J. Concise review: isoforms of OCT4 contribute to the confusing diversity in stem cell biology. Stem Cells 2010, 28:885-893. 2962909, 20333750.
10. Yasuhara N, Oka M, Yoneda Y. The role of the nuclear transport system in cell differentiation. Semin Cell Dev Biol 2009, 20:590-599. 10.1016/j.semcdb.2009.05.003, 19465141.
11. Jordan BA, Kreutz MR. Nucleocytoplasmic protein shuttling: the direct route in synapse-to-nucleus signaling. Trends Neurosci 2009, 32:392-401. 10.1016/j.tins.2009.04.001, 19524307.
12. Kau TR, Way JC, Silver PA. Nuclear transport and cancer: from mechanism to intervention. Nat Rev Cancer 2004, 4:106-117. 10.1038/nrc1274, 14732865.
13. Leung SW, Harreman MT, Hodel MR, Hodel AE, Corbett AH. Dissection of the karyopherin alpha nuclear localization signal (NLS)-binding groove: functional requirements for NLS binding. J Biol Chem 2003, 278:41947-41953. 10.1074/jbc.M307162200, 12917403.
14. Lange A, Mills RE, Lange CJ, Stewart M, Devine SE, Corbett AH. Classical nuclear localization signals: definition, function, and interaction with importin alpha. J Biol Chem 2007, 282:5101-5105.
15. Yasuhara N, Shibazaki N, Tanaka S, Nagai M, Kamikawa Y, Oe S, Asally M, Kamachi Y, Kondoh H, Yoneda Y. Triggering neural differentiation of ES cells by subtype switching of importin-alpha. Nat Cell Biol 2007, 9:72-79. 10.1038/ncb1521, 17159997.
16. Li X, Sun L, Jin Y. Identification of karyopherin-alpha 2 as an Oct4 associated protein. J Genet Genomics 2008, 35:723-728. 10.1016/S1673-8527(08)60227-1, 19103427.
17. Wang CI, Wang CL, Wang CW, Chen CD, Wu CC, Liang Y, Tsai YH, Chang YS, Yu JS, Yu CJ. Importin subunit alpha-2 is identified as a potential biomarker for non-small cell lung cancer by integration of the cancer cell secretome and tissue transcriptome. Int J Cancer 2011, 128:2364-2372. 10.1002/ijc.25568, 20658535.
18. Liang J, Wan M, Zhang Y, Gu P, Xin H, Jung SY, Qin J, Wong J, Cooney AJ, Liu D, Zhou SY. Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells. Nat Cell Biol 2008, 10:731-739. 10.1038/ncb1736, 18454139.
19. Tai MH, Chang CC, Kiupel M, Webster JD, Olson LK, Trosko JE. Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis. Carcinogenesis 2005, 26:495-502.
20. Kim JB, Sebastiano V, Wu GM, Arauzo-Bravo MJ, Sasse P, Gentile L, Ko K, Ruau D, Ehrich M, Van den BD, et al. Oct4-induced pluripotency in adult neural stem cells. Cell 2009, 136:411-419. 10.1016/j.cell.2009.01.023, 19203577.
21. Ezeh UI, Turek PJ, Reijo RA, Clark AT. Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma. Cancer 2005, 104:2255-2265. 10.1002/cncr.21432, 16228988.
22. Monk M, Holding C. Human embryonic genes re-expressed in cancer cells. Oncogene 2001, 20:8085-8091. 10.1038/sj.onc.1205088, 11781821.
23. Wang PX, Branch DR, Bali M, Schultz GA, Goss PE, Jin T. The POU homeodomain protein OCT3 as a potential transcriptional activator for fibroblast growth factor-4 (FGF-4) in human breast cancer cells. Biochem J 2003, 375:199-205. 10.1042/BJ20030579, 1223663, 12841847.
24. Gidekel S, Pizov G, Bergman Y, Pikarsky E. Oct-3/4 is a dose-dependent oncogenic fate determinant. Cancer Cell 2003, 4:361-370. 10.1016/S1535-6108(03)00270-8, 14667503.
25. Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S. Octamer 4 small interfering rna results in cancer stem cell-like cell apoptosis. Cancer Res 2008, 68:6533-6540. 10.1158/0008-5472.CAN-07-6642, 18701476.
26. Pan G, Qin B, Liu N, Scholer HR, Pei D. Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablation. J Biol Chem 2004, 279:37013-37020. 10.1074/jbc.M405117200, 15218026.
27. Köhler M, Speck C, Christiansen M, Bischoff FR, Prehn S, Haller H, Görlich D, Hartmann E. Evidence for distinct substrate specificities of importin-α family members in nuclear protein import. Mol Cell Biol 1999, 19:7782-7791. 84838, 10523667.
28. Yoneda Y. Nucleocytoplasmic protein traffic and its significance to cell function. Genes Cells 2000, 5:777-787. 10.1046/j.1365-2443.2000.00366.x, 11029654.
29. Goldfarb DS, Corbett AH, Mason DA, Harreman MT, Adam SA. Importin-α: a multipurpose nuclear-transport receptor. Trends Cell Biol 2004, 14:505-514. 10.1016/j.tcb.2004.07.016, 15350979.
30. Mortezavi A, Hermanns T, Seifert HH, Baumgartner M, Provenzano M, Sulser T, Burger M, Montani M, Ikenberg K, Hofstaedter F, Hartmann A, Jaggi R, Moch H, Kritiansen G, Wild PJ. KPNA2 expression is an independent adverse predictor of biochemical recurrence after radical prostatectomy. Clin Cancer Res 2011, 17:1111-1121. 10.1158/1078-0432.CCR-10-0081, 21220479.
31. Sakai M, Sohda M, Miyazaki T, Suzuki S, Sano A, Tanaka N, Inose T, Nakajima M, Kato H, Kuwano H. Significance of karyopherin-{alpha} 2 (KPNA2) expression in esophageal squamous cell carcinoma. Anticancer Res 2010, 30:851-856.
32. Dahl E, Kristiansen G, Gottlob K, Klaman I, Ebner E, Hinzmann B, Hermann K, Pilarsky C, Durst M, Klinkhammer-Schalke M, Blaszyk H, Knuechel R, Hartmann A, Rosenthal A, Wild PJ. Molecular profiling of laser-microdissected matched tumor and normal breast tissue identifies karyopherin alpha2 as a potential novel prognostic marker in breast cancer. Clin Cancer Res 2006, 12:3950-3960. 10.1158/1078-0432.CCR-05-2090, 16818692.
33. Gousias K, Becker AJ, Simon M, Niehusmann P. Nuclear karyopherin a2: a novel biomarker for infiltrative astrocytomas. J Neurooncol 2012, 109:545-553. 10.1007/s11060-012-0924-2, 22772608.
34. Noetzel E, Rose M, Bornemann J, Gajewski M, Knüchel R, Dahl E. Nuclear transport receptor karyopherin-a2 promotes malignant breast cancer phenotypes in vitro. Oncogene 2011, 31:2101-2114.