Light sheet microscopy of cleared mouse brains: Aberrations effects caused by refractive index mismatch

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 8804, Issue , 2013, Pages

  Silvestri, Ludovico ^a   Sacconi, Leonardo ^a,b   Pavone, Francesco S ^a,b,c,d  

^a UNIVERSITY OF FLORENCE   (Italy)

^b ISTITUTO NAZIONALE DI OTTICA   (Italy)

^c UNIVERSITY OF FLORENCE   (Italy)

^d International Center of Computational Neurophotonics (ICON)   (Italy)

Author keywords

aberration correction; brain imaging; fluorescence microscopy; light sheet microscopy; optical clearing

Indexed keywords

ABERRATION CORRECTION; BRAIN IMAGING; LIGHT-SHEET MICROSCOPIES; OPTICAL CLEARING; OUT-OF-FOCUS CONTRIBUTIONS; REFRACTIVE INDEX MATCHING; REFRACTIVE INDEX MISMATCH; THREE DIMENSIONAL IMAGING;

ABERRATIONS; FLUORESCENCE MICROSCOPY; NEUROIMAGING; OPTICAL TRANSFER FUNCTION; REFRACTIVE INDEX;

MAMMALS;

EID: 84884397004     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.2032488     Document Type: Conference Paper

References (8)

1. P. J. Keller, and H. U. Dodt, "Light sheet microscopy of living or cleared specimens, " Curr Opin Neurobiol, 22(1), 138-43 (2012)
2. J. Huisken, and D. Y. Stainier, "Selective plane illumination microscopy techniques in developmental biology, " Development, 136(12), 1963-75 (2009)
3. K. Becker, N. Jahrling, S. Saghafi et al., "Chemical clearing and dehydration of GFP expressing mouse brains, " PLoS One, 7(3), e33916 (2012)
4. H. U. Dodt, U. Leischner, A. Schierloh et al., "Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain, " Nat Methods, 4(4), 331-6 (2007)
5. L. Silvestri, A. Bria, L. Sacconi et al., "Confocal light sheet microscopy: micron-scale neuroanatomy of the entire mouse brain, " Opt Express, 20(18), 20582-98 (2012)
6. M. J. Booth, M. A. A. Neil, and T. Wilson, "Aberration correction for confocal imaging in refractive-indexmismatched media, " Journal of Microscopy-Oxford, 192, 90-98 (1998)
7. E. Baumgart, and U. Kubitscheck, "Scanned light sheet microscopy with confocal slit detection, " Opt Express, 20(19), 21805-14 (2012)
8. F. O. Fahrbach, and A. Rohrbach, "Propagation stability of self-reconstructing Bessel beams enables contrastenhanced imaging in thick media, " Nat Commun, 3, 632 (2012).