The lymphocyte transformation test for Borrelia detects active lyme borreliosis and verifies effective antibiotic treatment

Open Neurology Journal

Volumn 6, Issue SPEC.ISS.1, 2012, Pages 104-112

  von Baehr, Volker ^a   Doebis, Cornelia ^a   Volk, Hans Dieter ^b   von Baehr, Rüdiger ^a  

^a Institute for Medical Diagnostics   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Borrelia serology; Borreliosis; Diagnostics; Immune response; Lymphocyte transformation test; T cells

Indexed keywords

ANTIBIOTIC AGENT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M;

ADULT; AGED; ARTICLE; BORRELIA; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPSTEIN BARR VIRUS INFECTION; ERYTHEMA CHRONICUM MIGRANS; FEMALE; FOLLOW UP; HUMAN; IMMUNOBLOTTING; LYME DISEASE; LYMPHOCYTE TRANSFORMATION TEST; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; REACTIVE ARTHRITIS; RHEUMATOID ARTHRITIS; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; VALIDATION PROCESS;

EID: 84884324500     PISSN: None     EISSN: 1874205X     Source Type: Journal    
DOI: 10.2174/1874205X01206010104     Document Type: Article

References (35)

1. Steere AC. Lyme disease. N Engl J Med 2001;345:115-125.
2. Tewald F, Braun R. Durchführung und Interpretation serologischer Tests bei Verdacht auf Borrelieninfektion. Clin Lab 1998; 44: 897-902.
3. Dejmkova H, Hulinska D, Tegzova D, Pavelka K, Gatterova J, Vavrik P. Seronegative Lyme arthritis caused bei Borrelia garinii. Clin Rheumatol 2002; 21: 330-334.
4. Aguero-Rosenfeld ME, Wang G, Schwartz I, Wormser GP. Diagnosis of Lyme borreliosis. Clin Microbiol Rev 2005; 18: 484-509.
5. Wilske B, Fingerle V. Lyme-Borreliose Diagnostik. Mikrobiologe 2005; 15: 209-220.
6. Kalish RA, McHugh G,Granquist J, Ruthazer R, Steere AC. Persistence of immunoglobulin M or G antibody responses to Borrelia burgdorferi 10-20 years after active Lyme disease. Clin Infect Dis 2001; 33: 780-785.
7. Schütt C. Lymphozytentransformationstest (LTT). In:Friemel H, Ed. Immunologische Arbeitsmethoden. Stuttgart: G. Fischer Verlag Jena 1991; pp. 349-355.
8. Sigal LH, Moffat CM, Steere AC, Dwyer JM. Cellular immune findings in Lyme disease. Yale J Biol Med 1984; 57: 595-598.
9. Sigal LH, Steere AC, Freeman DH, Dwyer JM. Proliferative responses of mononuclear cells in Lyme disease. Arthritis Rheum 1986; 29: 761-769.
10. Dattwyler RJ, Volkman DJ, Luft BJ, Halperin JJ, Thomas J, Golightly MG. Seronegative Lyme disease. N Engl J Med 1988; 319: 1441-1446.
11. Dressler F, Yoshinari NH, Steere AC. The T-cell proliferative assay in the diagnosis of Lyme disease. Ann Intern Med 1991; 115: 533-539.
12. Krause A, Brade V, Schoerner C, Solbach W, Kalden JR, Bur-mester GRT cellproliferation induced by Borrelia burgdorferi in patients with Lyme borreliosis. Arthritis Rheum 1991; 34: 393-402.
13. Schempp C, Owsianoski M, Lange R, Gollick H. Comparison of Borrelia burgdorferi ultrasonicate and whole B. burgdorferi cells as a stimulus for T-cell proliferation and GM-CSF secretion in vitro. Zentralbl Bakteriol 1993; 279: 417-425.
14. Schempp C, Bocklage H, Owsianowski M, Lange R, Orfanos CE, Gollnick H. In vivo und in vitro Nachweis einer Borrelieninfektion bei einer morpheaähnlichen Hautveränderung mit negativer Borrelienserologie Hautarzt 1993; 44: 14-18.
15. Buechner SA, Lautenschläger S, Itin P, Bircher A, Erb P. Lymphoproliferative responses to Borrelia burgdorferi in patients with erythema migrans, acrodermatitis chronica atrophicans, lym-phadenosis benigna cutis, and morphea. Arch Dermatol 1995; 131: 673-677.
16. Breier F, Klade H, Stanek G, Poitschek C, Kirnbauer R, Dorda W, Aberer E. Lymphoproliferative responses to Borrelia burgdorferi in circumscribed scleroderma. Br J Dermatol 1996; 134: 285-291.
17. Rutkowski S, Busch DH, Huppertz HI. Lymphocyte proliferation assay in response to Borrelia burgdorferi in patients with Lyme ar-thritis:analysis of lymphocyte subsets. Rheumatol Int 1997; 17: 151-158.
18. Zoschke DC, Skemp AA, Defosse DL. Lymphoproliferative responses to Borrelia burgdorferi in Lyme disease. Ann Intern Med 1991; 114: 285-289.
19. Roessner K, Fikrig E, Russel JQ, Cooper SM, Flavell RA, Budd RC. Prominent T lymphocyte response to Borrelia burgdorferi from peripheral blood of unexposed donors. Eur J Immunol 1994; 24: 320-324.
20. Huppertz HI, Mösbauer S, Busch DH, Karch H. Lymphoprolifera-tive responses to Borrelia burgdorferi in the diagnosis of Lyme arthritis in children and adolescents. Eur J Pediatr 1996; 155: 297-302.
21. Breier F, Klade H, Stanek G. Lymphoproliferationstest bei kutanen Manifestationen der Lyme-Borreliose. Wien Med Wochenschr 1995; 27: 170-173.
22. Boyum A. Isolation of mononuclear cells and granulocytes from human blood. Scand J Cin Lab Invest 1968; (Suppl 97):77.
23. von Baehr V, Mayer W, Liebenthal C, von Baehr R, Bieger W, Volk HD. Improving the in vitro antigen specific T cell proliferation assay: the use of interferon-alpha to elicit antigen specific stimulation and decrease bystander proliferation. J Immunol Methods 2001; 251: 63-71.
24. Horowitz HH, Pavia CS, Bittker S, et al. Sustained cellular immune response to Borrelia burgdorferi: Lack of correlation with clinical presentation and serology. Clin Diagn Lab Immunol 1994; 1: 373-378.
25. Pohl-Koppe A, Kaunicnik A, Wilske B. Characterization of the cellular and humoral immune response to outer protein C and outer protein 17 in children with early disseminated Lyme borreliosis. Med Microbiol Immunol 2001; 189: 193-200.
26. Vaz A, Glickstein L, Field JA, et al. Cellular and humoral immune responses to Borrelia burgdorferi antigens in patients with culture-positive early Lyme disease. Infect Immun 2001; 69: 7437-7444.
27. Priem S, Burmester GR, Kamstadt T, Wolbart K, Rittig MG, Krause A. Detection of Borrelia burgdorferi by PCR in synovial membrane, but not synovial fluid from patients with persisting Lyme arthritis after antibiotic therapy. Ann Rheum Dis 1998; 57: 118-121.
28. Phillips SE, Mattman LH, Hulinska D, Moayad H. A proposal for the reliable culture of Borrelia burgdorferi from patients with chronic Lyme disease, even from those previously aggressively treated. Infektion 1998; 26: 364-367.
29. Oksi J, Marjamaki M, Nikoskelainen J, Viljanen MK. Borrelia burgdorferi detected by culture and PCR in clinical relapse of disseminated Lyme borreliosis. Ann Med 1999; 31: 225-232.
30. Honegr K, Hulinska D, Beran J, Dostal V, Havlasova J, Cermakova Z. Long term and repeated electron microskopy and PCR detection of Borrelia burgdorferi sensu lato after an antibiotic treatment. Cent Eur J Public Health 2004; 12: 6-11.
31. Valentine-Thon E, Ilsemann K, Sandkamp M. A novel lymphocyte transformation test (LTT-MELISA) for Lyme borreliosis. Diagn Microbiol Infect Dis 2007; 57: 27-34.
32. Connell TG, Rangaka MX, Curtis N, Wilkinson RJ. Quan-tiFERON-TB Gold: state of the art for the diagnosis of tuberculosis infection? Expert Rev Mol Diagn 2006; 6: 663-677.
33. Forsberg P, Ernerudh J, Ekerfelt C, Roberg M, Vrethem M, Bergström S. The outer surface proteins, of Lyme disease borrelia spirochetes stimulate T cells to secrete interferon-gamma (IFN-gamma): diagnostic and pathogenic implications. Clin Exp Immunol 1995; 101: 453-460.
34. Ekerfelt C, Forsberg P, Svenvic M, Roberg M, Bergström S, Ernerudh J. Asymptomatic borrelia-seropositive individuals display the same incidence of Borrelia-specific interferon-gamma (IFN-gamma)-secreting cells as patients with clinical Borrelia infection. Clin Exp Immunol 1999; 115: 498-502.
35. Ekerfelt C, Masreliez C, Svenvik M, Ernerudh J, Roberg M, Fors-berg P. Antibodies and T-cell reactivity to Borrelia burgdorferi in an asymptomatic population: a study of healthy blood donors in an inland town district in the south-east of Sweden. Scand J Infect Dis 2001; 33: 806-808.