Single-cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse escs

Cell Stem Cell

Volumn 13, Issue 1, 2013, Pages 23-29

  Faddah, Dina A ^a,b   Wang, Haoyi ^a   Cheng, Albert Wu ^a,b   Katz, Yarden ^a,b   Buganim, Yosef ^a   Jaenisch, Rudolf ^a,b  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA METHYLTRANSFERASE 3B; GREEN FLUORESCENT PROTEIN; KRUPPEL LIKE FACTOR 2; MESSENGER RNA; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2; BIOLOGICAL MARKER; HOMEODOMAIN PROTEIN; NANOG PROTEIN, MOUSE; PHOTOPROTEIN; RED FLUORESCENT PROTEIN;

3' UNTRANSLATED REGION; ALLELE; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL GROWTH; CHROMATIN ASSEMBLY AND DISASSEMBLY; EMBRYO; EMBRYONIC STEM CELL; GENE EXPRESSION; GENETIC CODE; GENETIC VARIABILITY; HETEROZYGOSITY; HOUSEKEEPING GENE; LOSS OF FUNCTION MUTATION; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SINGLE CELL ANALYSIS; START CODON; ANIMAL; C57BL MOUSE; CELL CULTURE; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENETICS; METABOLISM;

ANIMALS; BIOLOGICAL MARKERS; CELLS, CULTURED; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HOMEODOMAIN PROTEINS; IN SITU HYBRIDIZATION, FLUORESCENCE; LUMINESCENT PROTEINS; MICE; MICE, INBRED C57BL; PLURIPOTENT STEM CELLS; RNA, MESSENGER;

EID: 84884287890     PISSN: 19345909     EISSN: 18759777     Source Type: Journal    
DOI: 10.1016/j.stem.2013.04.019     Document Type: Article

References (22)

1. Buganim, Y., Faddah, D.A., Cheng, A.W., Itskovich, E., Markoulaki, S., Ganz, K., Klemm, S.L., van Oudenaarden, A., and Jaenisch, R. (2012). Single-cell expression analyses during cellular reprogramming reveal an early stochastic and a late hierarchic phase. Cell 150, 1209-1222.
2. Chambers, I., Colby, D., Robertson, M., Nichols, J., Lee, S., Tweedie, S., and Smith, A. (2003). Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113, 643-655.
3. Chambers, I., Silva, J., Colby, D., Nichols, J., Nijmeijer, B., Robertson, M., Vrana, J., Jones, K., Grotewold, L., and Smith, A. (2007). Nanog safeguards pluripotency and mediates germline development. Nature 450, 1230-1234.
4. Fidalgo, M., Faiola, F., Pereira, C.F., Ding, J., Saunders, A., Gingold, J., Schaniel, C., Lemischka, I.R., Silva, J.C., and Wang, J. (2012). Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming. Proc. Natl. Acad. Sci. USA 109, 16202-16207.
5. Filipczyk, A., Gkatzis, K., Fu, J., Hoppe, P., Lickert, H., Anastassiadis, K., and Schroeder, T. (2013). Biallelic expression of Nanog protein in mouse embryonic stem cells. Cell Stem Cell 13, this issue, 12-13.
6. Fischer, Y., Ganic, E., Ameri, J., Xian, X., Johannesson, M., and Semb, H. (2010). NANOG reporter cell lines generated by gene targeting in human embryonic stem cells. PLoS ONE 5, e12533.
7. Hatano, S.Y., Tada, M., Kimura, H., Yamaguchi, S., Kono, T., Nakano, T., Suemori, H., Nakatsuji, N., and Tada, T. (2005). Pluripotential competence of cells associated with Nanog activity. Mech. Dev. 122, 67-79.
8. Hayashi, K., Lopes, S.M., Tang, F., and Surani, M.A. (2008). Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states. Cell Stem Cell 3, 391-401.
9. Jaenisch, R., and Young, R. (2008). Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell 132, 567-582.
10. Kalmar, T., Lim, C., Hayward, P., Muñoz-Descalzo, S., Nichols, J., Garcia-Ojalvo, J., and Martinez Arias, A. (2009). Regulated fluctuations in nanog expression mediate cell fate decisions in embryonic stem cells. PLoS Biol. 7, e1000149.
11. Karwacki-Neisius, V., Göke, J., Osorno, R., Halbritter, F., Ng, J.H., Weiße, A.Y., Wong, F.C., Gagliardi, A., Mullin, N.P., Festuccia, N., et al. (2013). Reduced oct4 expression directs a robust pluripotent state with distinct signaling activity and increased enhancer occupancy by oct4 and nanog. Cell Stem Cell 12, 531-545.
12. MacArthur, B.D., Sevilla, A., Lenz, M., Müller, F.J., Schuldt, B.M., Schuppert, A.A., Ridden, S.J., Stumpf, P.S., Fidalgo, M., Ma'ayan, A., et al. (2012). Nanogdependent feedback loops regulate murine embryonic stem cell heterogeneity. Nat. Cell Biol. 14, 1139-1147.
13. Mitsui, K., Tokuzawa, Y., Itoh, H., Segawa, K., Murakami, M., Takahashi, K., Maruyama, M., Maeda, M., and Yamanaka, S. (2003). The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113, 631-642.
14. Miyanari, Y., and Torres-Padilla, M.E. (2012). Control of ground-state pluripotency by allelic regulation of Nanog. Nature 483, 470-473.
15. Navarro, P., Festuccia, N., Colby, D., Gagliardi, A., Mullin, N.P., Zhang, W., Karwacki-Neisius, V., Osorno, R., Kelly, D., Robertson, M., and Chambers, I. (2012). OCT4/SOX2-independent Nanog autorepression modulates heterogeneous Nanog gene expression in mouse ES cells. EMBO J. 31, 4547-4562.
16. Orkin, S.H., Wang, J., Kim, J., Chu, J., Rao, S., Theunissen, T.W., Shen, X., and Levasseur, D.N. (2008). The transcriptional network controlling pluripotency in ES cells. Cold Spring Harb. Symp. Quant. Biol. 73, 195-202.
17. Raj, A., van den Bogaard, P., Rifkin, S.A., van Oudenaarden, A., and Tyagi, S. (2008). Imaging individual mRNA molecules using multiple singly labeled probes. Nat. Methods 5, 877-879.
18. Silva, J., Barrandon, O., Nichols, J., Kawaguchi, J., Theunissen, T.W., and Smith, A. (2008). Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 6, e253.
19. Silva, J., Nichols, J., Theunissen, T.W., Guo, G., van Oosten, A.L., Barrandon, O., Wray, J., Yamanaka, S., Chambers, I., and Smith, A. (2009). Nanog is the gateway to the pluripotent ground state. Cell 138, 722-737.
20. Singh, A.M., Hamazaki, T., Hankowski, K.E., and Terada, N. (2007). A heterogeneous expression pattern for Nanog in embryonic stem cells. Stem Cells 25, 2534-2542.
21. Wray, J., Kalkan, T., and Smith, A.G. (2010). The ground state of pluripotency. Biochem. Soc. Trans. 38, 1027-1032.
22. Ying, Q.L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., and Smith, A. (2008). The ground state of embryonic stem cell self-renewal. Nature 453, 519-523.