May Salivary Gland Secretory Proteins from Hematophagous Leeches (Hirudo verbana) Reach Pharmacologically Relevant Concentrations in the Vertebrate Host?

PLoS ONE

Volumn 8, Issue 9, 2013, Pages

  Lemke, Sarah ^a   Müller, Christian ^a   Lipke, Elisabeth ^a   Uhl, Gabriele ^a   Hildebrandt, Jan Peter ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

SALIVA PROTEIN; SECRETORY PROTEIN;

ANIMAL TISSUE; ARTICLE; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; ENZYME LOCALIZATION; FEEDING; FEMALE; HIRUDO VERBANA; HISTOLOGY; HOST; HUMAN; LEECH; MOUSE; NONHUMAN; PLASMA VOLUME; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN TARGETING; SALIVARY GLAND; VERTEBRATE;

ANIMALS; DENSITOMETRY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; LEECHES; LIVER; MICE; MICROTOMY; MODELS, ANATOMIC; MUSCLE, SKELETAL; REFERENCE STANDARDS; SALIVARY GLANDS; SALIVARY PROTEINS AND PEPTIDES; SWINE; TISSUE FIXATION;

EID: 84884273322     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0073809     Document Type: Article

References (28)

1. Baskova IP, Zavalova LL, (2001) Proteinase inhibitors from the medicinal leech Hirudo medicinalis. Biochemistry (Mosc.) 66: 703-714.
2. Hildebrandt J-P, Lemke S, (2011) Small bite, large impact - saliva and salivary molecules in the medical leech, Hirudo medicinalis. Naturwissenschaften 98: 995-1008.
3. Sawyer RT (1986) Leech Biology and Behaviour 2: Feeding Biology, Ecology and Systematics. Oxford: Oxford University Press.
4. Whitaker IS, Izadi D, Oliver DW, Monteath G, Butler PE, (2004) Hirudo medicinalis and the plastic surgeon. Br J Plast Surg 57: 348-353.
5. Pilcher H, (2004) Medicinal leeches: stuck on you. Nature 432: 10-11.
6. Hach W, Scholz S, Hach-Wunderle V, (2005) Die Therapie mit Blutegeln in der Medizin und Gefäßmedizin. Gefäßchirurgie 10: 195-202.
7. Riede F, Koenen W, Goerdt S, Ehmke H, Faulhaber J, (2010) Medicinal leeches for the treatment of venous congestion and hematoma after plastic reconstructive surgery. J Dtsch Dermatol Ges 8: 881-888.
8. Yanes O, Villanueva J, Querol E, Aviles FX, (2005) Functional screening of serine protease inhibitors in the medical leech Hirudo medicinalis monitored by intensity fading MALDI-TOF MS. Mol Cell Proteomics 4: 1602-1613.
9. Min GS, Sarkar IN, Siddall ME, (2010) Salivary transcriptome of the North American medicinal leech, Macrobdella decora. J Parasitol 96: 1211-1221.
10. Ascenzi P, Amiconi G, Coletta M, Lupidi G, Menegatti E, et al. (1992) Binding of hirudin to human alpha, beta and gamma-thrombin. A comparative kinetic and thermodynamic study. J Mol Biol 225: 177-184.
11. Rigbi M, Orevi M, Eldor A, (1996) Platelet aggregation and coagulation inhibitors in leech saliva and their roles in leech therapy. Semin Thromb Hemost 22: 273-278.
12. Baskova IP, Zavalova LL, Basanova AV, Moshkovskii SA, Zgoda VG, (2004) Protein profiling of the medicinal leech salivary gland secretion by proteomic analytical methods. Biochemistry (Mosc) 69: 770-775.
13. Baskova IP, Zavalova LL, Kostrjukova ES, Titova GA, Lazarev VN, et al. (2007) Proteomic analysis methods for characterization of proteins from the salivary gland secretions of the medicinal leech during different seasons. Biochemistry (Mosc) 72: 219-225.
14. Haycraft JB, (1884) On the action of a secretion obtained from the medicinal leech on the coagulation of the blood. Proc R Soc Lond B 36: 478-487.
15. Nowak G, Schrör K, (2007) Hirudin - the long and stony way from an anticoagulant peptide in the saliva of medical leech to a recombinant drug and beyond. A historical piece. Thromb Haemost 98: 116-119.
16. Greinacher A, Warkentin TE, (2008) The direct thrombin inhibitor hirudin. Thromb Haemost 99: 819-29.
17. Zaidi SM, Jameel SS, Zaman F, Jilani S, Sultana A, et al. (2011) A systematic overview of the medical importance of sanguivorous leeches. Altern Med Rev 16: 59-65.
18. Gabe M (1968) Techniques histologiques. Paris: Masson and Cie.
19. Bradford MM, (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
20. Blum H, Beier H, Gross HJ, (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99.
21. Mann KH (1962) Leeches (Hirudinea). Oxford: Pergamon Press.
22. Lent CM, Fliegner KH, Freedman E, Dickinson MH, (1988) Ingestive behaviour and physiology of the medicinal leech. J Exp Biol 137: 513-527.
23. Oppler P (1904). Feinere Anatomie der im Kopf- und Halsteil von Hirudo vorkommenden Drüsen. PhD thesis, University of Bern.
24. Damas D, (1974) Etude histologique et histochemique des glandes salivaires de la sangsue medicinale, Hirudo medicinalis (Hirudinee, Gnathobdelle). Arch Zool Exp Gen 115: 279-292.
25. Damas D, (1972) Durcissement de la cuticule des machoires chez Hirudo medicinalis (Annelide, Hirudinee). Aboutissant aux structures dentaires etude histochemique et ultrastructurale. Arch Zool Exp Gen 113: 401-421.
26. Marshall CG, Lent CM, (1988) Excitability and secretory activity in the salivary gland cells of jawed leeches (Hirudinea: Gnathobdellida). J Exp Biol 137: 89-105.
27. Stone SR, Hofsteenge J, (1991) Basis of the reduced affinity of beta T- and gamma T-thrombin for hirudin. Biochemistry 30: 3950-3955.
28. Ascenzi P, Amiconi G, Bode W, Bolognesi M, Coletta M, et al. (1995) Proteinase inhibitors from the European medicinal leech Hirudo medicinalis: structural, functional and biomedical aspects. Mol Aspects Med 16: 215-313.