Comparison of computerized image analysis with traditional semiquantitative scoring of Perls' Prussian blue stained hepatic iron deposition

Toxicologic Pathology

Volumn 41, Issue 7, 2013, Pages 992-1000

  Hall, A Peter ^a   Davies, Wendy ^a   Stamp, Katie ^a   Clamp, Isabel ^a   Bigley, Alison ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

hemosiderin; image analysis; iron; Perls' Prussian Blue; PPB

Indexed keywords

FERRIC FERROCYANIDE; IRON;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; COMPARATIVE STUDY; FEMALE; HISTOCHEMISTRY; IMAGE ANALYSIS; MALE; NONHUMAN; PATHOLOGIST; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; SCORING SYSTEM;

HEMOSIDERIN; IMAGE ANALYSIS.; IRON; PERLS' PRUSSIAN BLUE; PPB;

ANIMALS; FEMALE; FERROCYANIDES; HISTOCYTOCHEMISTRY; IMAGE PROCESSING, COMPUTER-ASSISTED; IRON; LIVER; MALE; ORGAN SIZE; RATS; RATS, WISTAR; STATISTICS, NONPARAMETRIC;

EID: 84884241906     PISSN: 01926233     EISSN: 15331601     Source Type: Journal    
DOI: 10.1177/0192623313476576     Document Type: Article

References (16)

1. Brissot P., Bourel M., Herry D., Verger J. P., Messner M., Beaumont C., Regnouard F., Ferrand B., Simon M.. Assessment of liver iron content in 271 patients: A reevaluation of direct and indirect methods. Gastroenterology. 1981 ; 80: 557-65
2. Brown P. J., Whitbread T. J., Burden F. A., Bailey M., Carley K., Daniell L., Jones C., Bell N. J.. Haemosiderin deposition in Donkey (Equus asinus) livers: Comparison of quantitative histochemistry for iron and liver iron content. Res Vet Sci. 2011 ; 90: 284-87
3. Brunt E. M., Olynyk J. K., Britton R. S., Janney C. G., Di Bisceglie A. M., Bacon B. R.. Histological evaluation of iron in liver biopsies: Relationship to HFE mutations. Am J Gastroenterol. 2000 ; 95: 1788-93
4. Chua-anusorn W., Macey D. J., Webb J., de la Motte Hall P., St. Pierre T. G.. Effects of prolonged iron loading in the rat using both parenteral and dietary routes. Biometals. 1999 ; 12: 103-13
5. Churukian C. J., Techniques in neuropathology. In Theory and Practice of Histological Techniques. Bancroft J. D. Gamble M., ed. Edinburgh, UK: Churchill Livingstone ; 2008: 1-725.
6. Deugnier Y. M., Turlin B.. Pathology of hepatic iron overload. Semin Liver Dis. 2011 ; 31: 260-71
7. Deugnier Y. M., Turlin B., Powell L. W., Summers K. M., Moirand R., Fletcher L., Loreal O., Brissot P., Halliday J. W.. Differentiation between heterozygotes and homozygotes in genetic hemochromatosis by means of a histological hepatic iron index: A study of 192 cases. Hepatology. 1993 ; 17: 30-34
8. Farris A. B., Colvin R. B.. Renal interstitial fibrosis: Mechanisms and evaluation. Curr Opin Nephrol Hypertens. 2012 ; 21: 289-300
9. Harrison-Findik D. D.. Gender-related variations in iron metabolism and liver diseases. World J Hepatol. 2010 ; 2: 302-10
10. Masseroli M., Caballero T., O'Valle F., Del Moral R. M. G., Perez-Milena A., Del Moral R. G.. Automatic quantification of liver fibrosis: Design and validation of a new image analysis method. Comparison with semi-quantitative indexes of fibrosis. J Hepatol. 2000 ; 32: 453-64
11. Olynyk J., Hall P., Sallie R., Reed W., Shilkin K., Mackinnon M.. Computerized measurement of iron in liver biopsies: A comparison with biochemical iron measurement. Hepatology. 1990 ; 12: 26-30
12. Ortega L., Ladero J. M., Carreras M. P., Alvarez T., Taxonera C., Olivan M. P., Sanz-Esponera J., Diaz-Rubio M.. A computer-assisted morphometric quantitative analysis of iron overload in liver biopsies. A comparison with histological and biochemical methods. Pathol Res Pract. 2005 ; 201: 673-77
13. Pilette C., Rousselet M. C., Bedossa P., Chappard D., Oberti F., Rifflet H., Maiga M., Gallois Y., Cales P.. Histopathological evaluation of liver fibrosis: Image analysis is superior to observer score. J Hepatol. 1996 ; 25: 117
14. Ruifrok A. C., Johnston D. A.. Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol. 2001 ; 23: 291-99
15. Scheuer P. J., Williams R., Muir A. R.. Hepatic pathology in relatives of patients with haemochromatosis. J Pathol Bacteriol. 1962 ; 84: 53-64
16. Wright M., Thursz M., Pullen R., Thomas H., Goldin R.. Quantitative versus morphological assessment of liver fibrosis: Semi-quantitative scores are more robust than digital image fibrosis area estimation. Liver Int. 2003 ; 23: 28-34