Saccharification of cellulose by recombinant Rhodococcus opacus PD630 strains

Applied and Environmental Microbiology

Volumn 79, Issue 17, 2013, Pages 5159-5166

  Hetzler, Stephan ^a   Bröker, Daniel ^a   Steinbüchel, Alexander ^a,b  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYMETHYL CELLULOSE; CELLULASE ACTIVITY; CELLULOSE DEGRADATION; CELLULOSIC SUBSTRATES; MICROCRYSTALLINE CELLULOSE; RECOMBINANT STRAINS; RHODOCOCCUS OPACUS PD630; THERMOBIFIDA FUSCA;

BIOCHEMISTRY; CELLULOSE; EXPERIMENTS; GENE EXPRESSION; LIPIDS; SACCHARIFICATION; SUBSTRATES;

STRAIN;

CELLULOSE; CRYSTALLINITY; CULTIVATION; FATTY ACID; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC ENGINEERING; HYDROLYSIS; LIPID; PLASMID; PROKARYOTE; SUBSTRATE;

EID: 84884216713     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01214-13     Document Type: Article

References (45)

1. Rubin EM. 2008. Genomics of cellulosic biofuels. Nature 454:841-845.
2. Uthoff S, Bröker D, Steinbüchel A. 2009. Current state and perspectives of producing biodiesel-like compounds by biotechnology. Microb. Biotechnol. 2:551-565.
3. Fortman JL, Chhabra S, Mukhopadhyay A, Chou H, Lee TS, Steen E, Keasling JD. 2008. Biofuel alternatives to ethanol: pumping the microbial well. Trends Biotechnol. 26:375-381.
4. Chandel AK, Singh OV. 2011. Weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'biofuel.' Appl. Microbiol. Biotechnol. 89:1289-1303.
5. Bokinsky G, Peralta-Yahya PP, George A, Holmes BM, Steen EJ, Dietrich J, Soon Lee T, Tullman-Ercek D, Voigt CA, Simmons BA, Keasling JD. 2011. Synthesis of three advanced biofuels from ionic liquidpretreated switchgrass using engineered Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 108:19949-19954.
6. Regalbuto JR. 2009. Engineering. Cellulosic biofuels- got gasoline?. Science 325:822-824.
7. Limayem A, Ricke SC. 2012. Lignocellulosic biomass for bioethanol production: current perspectives, potential issues and future prospects. Prog. Energy Combust. Sci. 38:449-467.
8. Lynd LR, Laser MS, Bransby D, Dale BE, Davison B, Hamilton R, Himmel M, Keller M, McMillan JD, Sheehan J, Wyman CE. 2008. How biotech can transform biofuels. Nat. Biotechnol. 26:169-172.
9. Lynd LR, van Zyl WH, McBride JE, Laser M. 2005. Consolidated bioprocessing of cellulosic biomass: an update. Curr. Opin. Biotechnol. 16:577-583.
10. Luli GW, Jarboe L, Ingram LO. 2008. The development of ethanologenic bacteria for fuel ethanol production, p 129-137. In Wall JD, Harwood CS, Demain AL (ed), Bioenergy. ASM Press, Washington, DC.
11. Stephanopoulos G. 2007. Challenges in engineering microbes for biofuels production. Science 315:801-804.
12. Lee SK, Chou H, Ham TS, Lee TS, Keasling JD. 2008. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels. Curr. Opin. Biotechnol. 19:556-563.
13. Keasling JD. 2010. Manufacturing molecules through metabolic engineering. Science 330:1355-1358.
14. Fukuda H, Kondo A, Noda H. 2001. Biodiesel fuel production by transesterification of oils. J. Biosci. Bioeng. 92:405-416.
15. Wältermann M, Hinz A, Robenek H, Troyer D, Reichelt R, Malkus U, Galla HJ, Kalscheuer R, Stöveken T, von Landenberg P, Steinbüchel A. 2005. Mechanism of lipid-body formation in prokaryotes: how bacteria fatten up. Mol. Microbiol. 55:750-763.
16. Alvarez HM, Steinbüchel A. 2002. Triacylglycerols in prokaryotic microorganisms. Appl. Microbiol. Biotechnol. 60:367-376.
17. Wältermann M, Luftmann H, Baumeister D, Kalscheuer R, Steinbüchel A. 2000. Rhodococcus opacus strain PD630 as a new source of high-value single-cell oil? Isolation and characterization of triacylglycerols and other storage lipids. Microbiology 146:1143-1149.
18. Schlegel HG, Kaltwasser H, Gottschalk G. 1961. Ein Submersverfahren zur Kultur wasserstoffoxidierender Bakterien: wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 38:209-222.
19. Waksman SA. 1961. The Actinomycetes, vol II. Classification, identification, and descriptions of genera and species. The Williams & Wilkins Co., Baltimore, MD.
20. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
21. Birnboim HC, Doly J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
22. Tauch A, Kirchner O, Löffler B, Götker S, Pühler A, Kalinowski J. 2002. Efficient electrotransformation of Corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1. Curr. Microbiol. 45:362-367.
23. Triccas JA, Parish T, Britton WJ, Gicquel B. 1998. An inducible expression system permitting the efficient purification of a recombinant antigen from Mycobacterium smegmatis. FEMS Microbiol. Lett. 167:151-156.
24. Hanahan D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
25. Kalscheuer R, Arenskötter M, Steinbüchel A. 1999. Establishment of a gene transfer system for Rhodococcus opacus PD630 based on electropora- tion and its application for recombinant biosynthesis of poly(3- hydroxyalkanoic acids). Appl. Microbiol. Biotechnol. 52:508-515.
26. Béguin P. 1983. Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas. Anal. Biochem. 131:333-336.
27. Holder JW, Ulrich JC, DeBono AC, Godfrey PA, Desjardins CA, Zucker J, Zeng Q, Leach AL, Ghiviriga I, Dancel C, Abeel T, Gevers D, Kodira CD, Desany B, Affourtit JP, Birren BW, Sinskey AJ. 2011. Comparative and functional genomics of Rhodococcus opacus PD630 for biofuels development. PLoS Genet. 7:e1002219. doi:10.1371/journal.pgen.1002219.
28. Wong WK, Gerhard B, Guo ZM, Kilburn DG, Warren AJ, Miller RC, Jr. 1986. Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi. Gene 44:315-324.
29. Meinke A, Braun C, Gilkes NR, Kilburn DG, Miller RC, Jr, Warren RA. 1991. Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi. J. Bacteriol. 173:308-314.
30. Coutinho JB, Moser B, Kilburn DG, Warren RA, Miller RC, Jr. 1991. Nucleotide sequence of the endoglucanase C gene (cenC) of Cellulomonas fimi, its high-level expression in Escherichia coli, and characterization of its products. Mol. Microbiol. 5:1221-1233.
31. Wilson DB. 2004. Studies of Thermobifida fusca plant cell wall degrading enzymes. Chem. Rec. 4:72-82.
32. Meinke A, Gilkes NR, Kwan E, Kilburn DG, Warren RA, Miller RC, Jr. 1994. Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a --1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II. Mol. Microbiol. 12:413-422.
33. O'Neill G, Goh SH, Warren RA, Kilburn DG, Miller RC, Jr. 1986. Structure of the gene encoding the exoglucanase of Cellulomonas fimi. Gene 44:325-330.
34. Hänisch J, Wältermann M, Robenek H, Steinbüchel A. 2006. The Ralstonia eutropha H16 phasin PhaP1 is targeted to intracellular triacylglycerol inclusions in Rhodococcus opacus PD630 and Mycobacterium smegmatis mc2155, and provides an anchor to target other proteins. Microbiology 152:3271-3280.
35. Smith MA, Bidochka MJ. 1998. Bacterial fitness and plasmid loss: the importance of culture conditions and plasmid size. Can. J. Microbiol. 44:351-355.
36. Guo Z, Arfman N, Ong E, Gilkes NR, Kilburn DG, Warren RAJ, Miller RC, Jr. 1988. Leakage of Cellulomonas fimi cellulases from Escherichia coli. FEMS Microbiol. Lett. 49:279-283.
37. Nielsen H, Engelbrecht J, Brunak S, von Heijne G. 1997. A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Int. J. Neural Syst. 8:581- 599.
38. Tomme P, Kwan E, Gilkes NR, Kilburn DG, Warren RA. 1996. Characterization of CenC, an enzyme from Cellulomonas fimi with both endoand exoglucanase activities. J. Bacteriol. 178:4216-4223.
39. Teeri TT. 1997. Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Trends Biotechnol. 15:160-167.
40. Igarashi K, Uchihashi T, Koivula A, Wada M, Kimura S, Okamoto T, Penttila M, Ando T, Samejima M. 2011. Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science 333:1279 - 1282.
41. Irwin DC, Zhang S, Wilson DB. 2000. Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca. Eur. J. Biochem. 267:4988-4997.
42. Alvarez HM, Mayer F, Fabritius D, Steinbüchel A. 1996. Formation of intracytoplasmic lipid inclusions by Rhodococcus opacus strain PD630. Arch. Microbiol. 165:377-386.
43. Stackebrandt E, Kandler O. 1979. Taxonomy of the genus Cellulomonas, based on phenotypic characters and deoxyribonucleic acid-deoxyribonucleic acid homology, and proposal of seven neotype strains. Int. J. Syst. Evol. Microbiol. 29:273-282.
44. McCarthy AJ, Cross T. 1984. A taxonomic study of Thermomonospora and other monosporic actinomycetes. Microbiology 130:5-25.
45. Hetzler S, Steinbüchel A. 2013. Establishment of cellobiose utilization for lipid production in Rhodococcus opacus PD630. Appl. Environ. Microbiol. 79:3122-3125.