Extending the cellulosome paradigm: The modular clostridium thermocellum cellulosomal serpin pinA is a broad-spectrum inhibitor of subtilisin-like proteases

Applied and Environmental Microbiology

Volumn 79, Issue 19, 2013, Pages 6173-6175

  Cuív, Páraic Ó ^a   Gupta, Rajesh ^a   Goswami, Hareshwar P ^a   Morrisona, Mark ^b  

^a CSIRO   (Australia)

^b OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULOSOMES; CLOSTRIDIUM THERMOCELLUM; INHIBITORY ACTIVITY; SERINE PROTEASE INHIBITOR;

AMINO ACIDS; CLOSTRIDIUM;

GENE ENCODING;

BACTERIOLOGY; BACTERIUM; ENZYME ACTIVITY; INHIBITOR;

CELLULASE; CELLULOSOME, CLOSTRIDIUM; MULTIENZYME COMPLEX; SERINE PROTEINASE INHIBITOR; SUBTILISIN;

ARTICLE; CHEMISTRY; CLOSTRIDIUM THERMOCELLUM; DRUG ANTAGONISM; ENZYMOLOGY; METABOLISM; PROTEIN STABILITY;

CELLULASE; CLOSTRIDIUM THERMOCELLUM; MULTIENZYME COMPLEXES; PROTEIN STABILITY; SERPINS; SUBTILISIN;

EID: 84884198926     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01912-13     Document Type: Article

References (22)

1. Meguro H, Morisaka H, Kuroda K, Miyake H, Tamaru Y, Ueda M. 2011. Putative role of cellulosomal protease inhibitors in Clostridium cellulovorans based on gene expression and measurement of activities. J. Bacteriol. 193:5527-5530.
2. Kang S, Barak Y, Lamed R, Bayer EA, Morrison M. 2006. The functional repertoire of prokaryote cellulosomes includes the serpin superfamily of serine proteinase inhibitors. Mol. Microbiol. 60:1344-1354.
3. Fendri I, Tardif C, Fierobe H-P, Lignon S, Valette O, Pagès S, Perret S. 2009. The cellulosomes from Clostridium cellulolyticum. FEBS J. 276: 3076-3086.
4. Steenbakkers PJM, Irving JA, Harhangi HR, Swinkels WJC, Akhmanova A, Dijkerman R, Jetten MSM, van der Drift C, Whisstock JC, Op den Camp HJM. 2008. A serpin in the cellulosome of the anaerobic fungus Piromyces sp. strain E2. Mycol. Res. 112:999-1006.
5. Dafforn TR, Pike RN, Bottomley SP. 2004. Physical characterization of serpin conformations. Methods 32:150-158.
6. Zhang Q, Buckle AM, Law RHP, Pearce MC, Cabrita LD, Lloyd GJ, Irving JA, Smith AI, Ruzyla K, Rossjohn J, Bottomley SP, Whisstock JC. 2007. The N terminus of the serpin, tengpin, functions to trap the metastable native state. EMBO Rep. 8:658-663.
7. Kataeva IA, Seidel RD, Shah IIIA, West LT, Li X-L, Ljungdahl LG. 2002. The fibronectin type 3-like repeat from the Clostridium thermocellum cellobiohydrolase CbhA promotes hydrolysis of cellulose by modifying its surface. Appl. Environ. Microbiol. 68:4292-4300.
8. Kataeva IA, Blum DL, Li X-L, Ljungdahl LG. 2001. Do domain interactions of glycosyl hydrolases from Clostridium thermocellum contribute to protein thermostability?. Protein Eng. 14:167-172.
9. Kataeva IA, Brewer JM, Uversky VN, Ljungdahl LG. 2005. Domain coupling in a multimodular cellobiohydrolase CbhA from Clostridium thermocellum. FEBS Lett. 579:4367-4373.
10. Kataeva IA, Uversky VN, Brewer JM, Schubot F, Rose JP, Wang BC, Ljungdahl LG. 2004. Interactions between immunoglobulin-like and catalytic modules in Clostridium thermocellum cellulosomal cellobiohydrolase CbhA. Protein Eng. Des. Sel. 17:759-769.
11. Wassenberg D, Schurig H, Liebl W, Jaenicke R. 1997. Xylanase XynA from the hyperthermophilic bacterium Thermotoga maritima: structure and stability of the recombinant enzyme and its isolated cellulose-binding domain. Protein Sci. 6:1718-1726.
12. Mrkonjic Fuka M, Engel M, Gattinger A, Bausenwein U, Sommer M, Munch JC, Schloter M. 2008. Factors influencing variability of proteolytic genes and activities in arable soils. Soil Biol. Biochem. 40:1646-1653.
13. Gabor E, Niehaus F, Aehle W, Eck J. 2012. Zooming in on metagenomics: molecular microdiversity of subtilisin Carlsberg in soil. J. Mol. Biol. 418:16-20.
14. Kaiser C, Koranda M, Kitzler B, Fuchslueger L, Schnecker J, Schweiger P, Rasche F, Zechmeister-Boltenstern S, Sessitsch A, Richter A. 2010. Belowground carbon allocation by trees drives seasonal patterns of extracellular enzyme activities by altering microbial community composition in a beech forest soil. New Phytol. 187:843-858.
15. Maurer KH. 2004. Detergent proteases. Curr. Opin. Biotechnol. 15:330-334.
16. Jany K-D, Lederer G, Mayer B. 1986. Amino acid sequence of proteinase K from the mold Tritirachium album Limber: Proteinase K-a subtilisinrelated enzyme with disulfide bonds. FEBS Lett. 199:139-144.
17. Gunkel FA, Gassen HG. 1989. Proteinase K from Tritirachium album Limber. Characterization of the chromosomal gene and expression of the cDNA in Escherichia coli. Eur. J. Biochem. 179:185-194.
18. Gettins PG, Olson ST. 2009. Exosite determinants of serpin specificity. J. Biol. Chem. 284:20441-20445.
19. Betzel C, Klupsch S, Branner S, Wilson KS. 1996. Crystal structures of the alkaline proteases savinase and esperase from Bacillus lentus. Adv. Exp. Med. Biol. 379:49-61.
20. Pähler A, Banerjee A, Dattagupta JK, Fujiwara T, Lindner K, Pal GP, Suck D, Weber G, Saenger W. 1984. Three-dimensional structure of fungal proteinase K reveals similarity to bacterial subtilisin. EMBO J. 3:1311-1314.
21. Roberts SB, Gowen CM, Brooks JP, Fong SS. 2010. Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production. BMC Syst. Biol. 4:31. doi:10.1186/1752-0509-4-31.
22. Johnson EA, Madia A, Demain AL. 1981. Chemically defined minimal medium for growth of the anaerobic cellulolytic thermophile Clostridium wthermocellum. Appl. Environ. Microbiol. 41:1060-1062.