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Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 938, Issue , 2013, Pages 14-21

Purification of antibodies by precipitating impurities using Polyethylene Glycol to enable a two chromatography step process

(4) Giese, Glen a Myrold, Adam a Gorrell, Jeffrey a Persson, Josefine a

a GENENTECH INC (United States)

Author keywords

Antibody; PEG; Precipitation; Purification

Indexed keywords

CHROMATOGRAPHY COLUMNS; HIGH MOLECULAR WEIGHT; ISOELECTRIC POINT (PI); PEG PRECIPITATIONS; POLYETHYLENE GLYCOL (PEG); PROTEIN CONCENTRATIONS; PURIFICATION PROCESS; RECOMBINANT MONOCLONAL ANTIBODY;

ANTIBODIES; COLUMN CHROMATOGRAPHY; MONOCLONAL ANTIBODIES; POLYETHYLENE GLYCOLS; PRECIPITATION (CHEMICAL); PROTEINS; VIRUSES;

PURIFICATION;

CELL PROTEIN; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G4; LIGAND; MACROGOL; MONOCLONAL ANTIBODY; PROTEIN A;

ANION EXCHANGE CHROMATOGRAPHY; ANTIBODY BLOOD LEVEL; ARTICLE; CATION EXCHANGE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DOWNSTREAM PROCESSING; ENDOGENOUS VIRUS; HOST CELL; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC POINT; MOLECULAR WEIGHT; NONHUMAN; PARVOVIRUS; PH; PRECIPITATION; PRIORITY JOURNAL; PURIFICATION; TEMPERATURE;

AIEX; ANION EXCHANGE; ANTIBODY; CATION EXCHANGE; CIEX; COLUMN VOLUME; CV; DESIGN OF EXPERIMENTS; DOE; HCP; HIGH MOLECULAR WEIGHT SPECIES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HMWS; HOST CELL PROTEIN; HPLC; LOG REDUCTION VALUE; LRV; MAB; MINUTE MOUSE VIRUS; MMV; MONOCLONAL ANTIBODY; PEG; POLYETHYLENE GLYCOL; PRECIPITATION; PURIFICATION; SEC; SIZE EXCLUSION CHROMATOGRAPHY;

ANIMALS; ANTIBODIES, MONOCLONAL; CHEMICAL PRECIPITATION; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; POLYETHYLENE GLYCOLS; RECOMBINANT PROTEINS; TEMPERATURE; VIRUSES;

EID: 84884184342 PISSN: 15700232 EISSN: 1873376X Source Type: Journal
DOI: 10.1016/j.jchromb.2013.08.029 Document Type: Article

Times cited : (35)

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