ULK1 Regulates Melanin Levels in MNT-1 Cells Independently of mTORC1

PLoS ONE

Volumn 8, Issue 9, 2013, Pages

  Kalie, Eyal ^a   Razi, Minoo ^a   Tooze, Sharon A ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; MAMMALIAN TARGET OF RAPAMYCIN COMPLEX 1; MELANIN; MICROPHTHALMIA ASSOCIATED TRANSCRIPTION FACTOR; MONOPHENOL MONOOXYGENASE; PROTEIN ATG13; PROTEIN FIP200; PROTEIN ULK1; UNCLASSIFIED DRUG;

ARTICLE; AUTOPHAGY; CELL STRAIN MNT 1; CONTROLLED STUDY; GENE SILENCING; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MELANOGENESIS; MELANOSOME; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS INHIBITION; REGULATORY MECHANISM; SIGNAL TRANSDUCTION;

BLOTTING, WESTERN; CELL LINE; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MELANINS; MULTIPROTEIN COMPLEXES; PROTEIN-SERINE-THREONINE KINASES; REAL-TIME POLYMERASE CHAIN REACTION; TOR SERINE-THREONINE KINASES;

EID: 84884181586     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0075313     Document Type: Article

References (26)

1. Dell'Angelica EC, Mullins C, Caplan S, Bonifacino JS, (2000) Lysosome-related organelles. FASEB J 14: 1265-1278. doi:10.1096/fj.14.10.1265. PubMed: 10877819.
2. Raposo G, Marks MS, Cutler DF, (2007) Lysosome-related organelles: driving post-Golgi compartments into specialisation. Curr Opin Cell Biol 19: 394-401. doi:10.1016/j.ceb.2007.05.001. PubMed: 17628466.
3. Raposo G, Tenza D, Murphy DM, Berson JF, Marks MS, (2001) Distinct protein sorting and localization to premelanosomes, melanosomes, and lysosomes in pigmented melanocytic cells. J Cell Biol 152: 809-824. doi:10.1083/jcb.152.4.809. PubMed: 11266471.
4. Berson JF, Harper DC, Tenza D, Raposo G, Marks MS, (2001) Pmel17 initiates premelanosome morphogenesis within multivesicular bodies. Mol Biol Cell 12: 3451-3464. doi:10.1091/mbc.12.11.3451. PubMed: 11694580.
5. Hurbain I, Geerts WJ, Boudier T, Marco S, Verkleij AJ, et al. (2008) Electron tomography of early melanosomes: implications for melanogenesis and the generation of fibrillar amyloid sheets. Proc Natl Acad Sci U S A 105: 19726-19731. doi:10.1073/pnas.0803488105. PubMed: 19033461.
6. Kushimoto T, Basrur V, Valencia J, Matsunaga J, Vieira WD, et al. (2001) A model for melanosome biogenesis based on the purification and analysis of early melanosomes. Proc Natl Acad Sci U S A 98: 10698-10703. doi:10.1073/pnas.191184798. PubMed: 11526213.
7. Sitaram A, Marks MS, (2012) Mechanisms of protein delivery to melanosomes in pigment cells. Physiology (Bethesda) 27: 85-99. doi:10.1152/physiol.00043.2011. PubMed: 22505665.
8. Bultema JJ, Ambrosio AL, Burek CL, Pietro, Di (2012) BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles. J Biol Chem 287: 19550-19563. doi:10.1074/jbc.M112.351908. PubMed: 22511774.
9. Theos AC, Tenza D, Martina JA, Hurbain I, Peden AA, et al. (2005) Functions of adaptor protein (AP)-3 and AP-1 in tyrosinase sorting from endosomes to melanosomes. Mol Biol Cell 16: 5356-5372. doi:10.1091/mbc.E05-07-0626. PubMed: 16162817.
10. Ho H, Ganesan AK, (2011) The pleiotropic roles of autophagy regulators in melanogenesis. Pigment Cell. Melanoma Res 24: 595-604.
11. Choi AMK, Ryter SW, Levine B, (2013) Autophagy in Human Health and Disease. N Engl J Med 368: 651-662. doi:10.1056/NEJMra1205406. PubMed: 23406030.
12. Tooze SA, Jefferies HB, Kalie E, Longatti A, McAlpine FE, et al. (2010) Trafficking and signaling in mammalian autophagy. IUBMB Life 62: 503-508. doi:10.1002/iub.334. PubMed: 20552641.
13. Ganesan AK, Ho H, Bodemann B, Petersen S, Aruri J, et al. (2008) Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells. PLOS Genet 4: e1000298. PubMed: 19057677.
14. Ho H, Kapadia R, Al-Tahan S, Ahmad S, Ganesan AK, (2011) WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem 286: 12509-12523. doi:10.1074/jbc.M110.200543. PubMed: 21317285.
15. Chan EY, Kir S, Tooze SA, (2007) siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem 282: 25464-25474. doi:10.1074/jbc.M703663200. PubMed: 17595159.
16. Wong P-M, Puente C, Ganley IG, Jiang X, (2013) The ULK1 complex: Sensing nutrient signals for autophagy activation. Autophagy 9: 124-137. doi:10.4161/auto.23323. PubMed: 23295650.
17. Cuomo M, Nicotra MR, Apollonj C, Fraioli R, Giacomini P, et al. (1991) Production and Characterization of the Murine Monoclonal Antibody 2G10 to a Human T4-Tyrosinase Epitope. J Investig Dermatol 96: 446-451. doi:10.1111/1523-1747.ep12470092. PubMed: 1706743.
18. Chan EY, Longatti A, McKnight NC, Tooze SA, (2009) Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism. Mol Cell Biol 29: 157-171. doi:10.1128/MCB.01082-08. PubMed: 18936157.
19. Wasmeier C, Romao M, Plowright L, Bennett DC, Raposo G, et al. (2006) Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes. J Cell Biol 175: 271-281. doi:10.1083/jcb.200606050. PubMed: 17043139.
20. Dunlop EA, Hunt DK, Acosta-Jaquez HA, Fingar DC, Tee AR, (2011) ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding. Autophagy 7: 737-747. doi:10.4161/auto.7.7.15491. PubMed: 21460630.
21. Jung CH, Seo M, Otto NM, Kim DH, (2011) ULK1 inhibits the kinase activity of mTORC1 and cell proliferation. Autophagy 7: 1212-1221. doi:10.4161/auto.7.10.16660. PubMed: 21795849.
22. Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, et al. (2012) Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8: 445-544. doi:10.4161/auto.19496. PubMed: 22966490.
23. Bellei B, Maresca V, Flori E, Pitisci A, Larue L, et al. (2010) p38 regulates pigmentation via proteasomal degradation of tyrosinase. J Biol Chem 285: 7288-7299. doi:10.1074/jbc.M109.070573. PubMed: 20053998.
24. Hasegawa T, (2010) Tyrosinase-expressing neuronal cell line as in vitro model of Parkinson's disease. Int J Mol Sci 11: 1082-1089. doi:10.3390/ijms11031082. PubMed: 20480001.
25. McAlpine F, Williamson LE, Tooze SA, Chan EYW, (2013) Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2. Autophagy 9: 361-373. doi:10.4161/auto.23066. PubMed: 23291478.
26. Murase D, Hachiya A, Takano K, Hicks R, Visscher MO, et al. (2013) Autophagy Plays a Significant Role in Determining Skin Color by Regulating Melanosome Degradation in Keratinocytes. J Invest Dermatol.