Lentiviral transduction of neuronal cells

Methods in Molecular Biology

Volumn 1078, Issue , 2013, Pages 141-146

  Wollebo, Hassen S ^a   Woldemichaele, Baheru ^a   White, Martyn K ^a  

^a TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Lentiviral vectors; Primary human fetal astrocytes; Transduction

Indexed keywords

LENTIVIRUS VECTOR; LUCIFERASE;

ARTICLE; ASTROCYTE; CELL DENSITY; EXPRESSION VECTOR; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LENTIVIRINAE; NERVE CELL; PLASMID; PRIMARY CELL CULTURE; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; TARGET CELL; VIRUS PARTICLE;

ASTROCYTES; FETUS; GENETIC VECTORS; HEK293 CELLS; HIV-1; HUMANS; LENTIVIRUS; NEURONS; TRANSDUCTION, GENETIC; TRANSFECTION;

EID: 84884173073     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-640-5_12     Document Type: Article

References (12)

1. Pfeifer A, Verma IM (2001) Gene therapy: promises and problems. Annu Rev Genomics Hum Genet 2:177-211
2. Denning W, Das S, Guo S et al (2013) Optimization of the transductional efficiency of lentiviral vectors: effect of sera and polycations. Mol Biotechnol 53(3):308-314
3. Mátrai J, Chuah MK, Vanden Driessche T (2010) Recent advances in lentiviral vector development and application. Mol Ther 18:477-490
4. Sakuma T, Barry MA, Ikeda Y (2012) Lentiviral vectors: basic to translation. Biochem J 443:603-618
5. van Hooijdonk LW, Ichwan M, Dijkmans TF et al (2009) Lentivirus-mediated transgene delivery to the hippocampus reveals sub-field specific differences in expression. BMC Neurosci 10:2
6. Naldini L, Blomer U, Gage FH et al (1996) Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brain injected with a lentiviral vector. Proc Natl Acad Sci U S A 93:11382-11388
7. Naldini L, Blomer U, Gallay P et al (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272:263-267
8. Logan AC, Lutzko C, Kohn B (2002) Advances in lentiviral vector design for gene-modification of hematopoietic stem cells. Curr Opin Biotechnol 13:429-436
9. Varma NR, Janic B, Ali MM et al (2011) Lentiviral Based Gene transduction and promoter studies in Human Hematopoietic Stem Cells (hHSCs). J Stem Cells Regen Med 7:41-53
10. Radhakrishnan S, Otte J, Enam S et al (2003) JC virus-induced changes in cellular gene expression in primary human astrocytes. J Virol 77:10637-10644
11. Cole R, de Vellis J (1997) Astrocyte and oligodendrocyte culture. In: Fedoroff S, Richardson A (eds) Protocols for neural cell culture. Humana, Totowa, NJ
12. Yong VW, Antel JP (1997) Culture of glial cells from human brain biopsies. In: Fedoroff S, Richardson A (eds) Protocol for neural cell culture. Humana, Totowa, NJ