Phenotypic and genotypic methods for detection of extended spectrum β lactamase producing Escherichia coli and Klebsiella pneumoniae isolated from Ventilator Associated Pneumonia

Journal of Clinical and Diagnostic Research

Volumn 7, Issue 9, 2013, Pages 1975-1978

  Krishnamurthy, Veena ^a   Vijaykumar, G S ^b   Sudeepa Kumar, M ^a   Prashanth, H V ^a   Prakash, R ^a   Nagaraj, E R ^a  

^a SRI SIDDHARTHA MEDICAL COLLEGE   (India)

^b JSS MEDICAL COLLEGE   (India)

Author keywords

BlaCTX M gene; BlaSHV gene; BlaTEM gene; Extended Spectrum Beta Lactamase; Polymerase Chain Reaction; Ventilator Associated Pneumonia

Indexed keywords

BETA LACTAMASE CTX M; BETA LACTAMASE SHV; BETA LACTAMASE TEM 1; CEFOTAXIME; CLAVULANIC ACID; EXTENDED SPECTRUM BETA LACTAMASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATION; BLA CTX M GENE; BLA SHV GENE; BLA TEM1 GENE; BRONCHIAL ASPIRATION PROCEDURE; CONTROLLED STUDY; DISK DIFFUSION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE IDENTIFICATION; GENOTYPING TECHNIQUE; HUMAN; KLEBSIELLA PNEUMONIAE; MAJOR CLINICAL STUDY; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; VENTILATOR ASSOCIATED PNEUMONIA;

EID: 84883893271     PISSN: 2249782X     EISSN: 0973709X     Source Type: Journal    
DOI: 10.7860/JCDR/2013/6544.3376     Document Type: Article

References (26)

1. Chastre J, Fagon JY. Ventilator associated pneumonia. Am J Respir Crit Care Med. 2002; 165: 867-903.
2. Safdar N, Dezfulian C, Collard HR, Saint S. Clinical and economic consequences of ventilator associated pneumonia: A systematic review. Crit care Med. 2005; 33: 2184-93.
3. Rello J, Ollendrof DA, Oster G, Vera-Llonch M, Redman R. Epidemology and outcomes of ventilator associated pneumonia in a large US database. Chest. 2002; 122: 2115-21.
4. Kumar RS, Lakshmi V, Rajagopal R. Occurence of extended spectrum Betalactamase among Enterobacteriaceae spp. isolated at a tertiary care institute. Indian J Med Microbiol. 2006; 24(3): 208-11.
5. Bazzaz BS, Naderinasab M, Mohamadpoor AH, Farshadzadeh Z, Ahmadi S, Yousefi F. Escherichia coli and Klebsiella pneumoniae among clinical isolates from a general hospital in Iran. Acta Microbiol Immunol Hung. 2009; 56(1): 89-99.
6. Asgar AH. Prevalence and Potential Types of Extended Spectrum β-Lactamases among Escherichia coli and Klebsiella species in Makkah Hospitals. Oxford Research Forum Journal. 2007; 2(4): 47-55.
7. Al-Algamy MHM, Shibl AM, Tawfik AF. Prevalence and molecular characterization of extended-spectrum β-lactamase-producing Klebsiella pneumoniae in Riyadh, Saudi Arabia. Ann Saudi Med. 2009; 29(4): 253-07.
8. Dwivedi M, Mishra A, Azim A, Singh RK, Baroni AK, Prasad KN, et al. Ventilatorassociated pneumonia caused by carbaoenem-resistant Enterobacteriaceae carrying multiple metallo-beta lactamase genes. Indian J Pathol Microbiol. 2009; 52(3):339-42.
9. Rajasekhar T, Anuradha K, Suhasini T, Lakshmi V. The role of quantitative cultures of non-bronchoscopic samples in ventilator-associated pneumonia. Indian J Med Microbiol. 2006; 24(2): 107-13.
10. Baselski VS, El-Torky M, Coalson JJ, Grifin JP. The Standardization of Criteria for processing and Interpretation of Laboratory specimens in patients with suspected ventilator associated. Chest. 1992; 102(5): 571-79.
11. Collee JG, Miles RS. Tests for Identification of Bacteria. In: Collee JG, Duduid JP, Fraser AG, Matmion, editors. Mackie and McCartney, Practical Medical Microbiology. 14th ed. New York, Longman Singapore publishing; 1996, p.141-60.
12. Clinical Laboratory Standard Institute. Performance Standards for antimicrobial disk susceptibility tests approved standard. 9th ed CLSI document M2-49, CLSI; Wayne PA. 2012.
13. Jarlier V, Nicholas NH, Fourier G, Phillippon. Extended broad spectrum β lactamase conferring transferable resistance to newer β lactam agents in Enterobacteriaceae, hospital prevalence and susceptibility patterns. Rev Infect Dis. 1988; 10(4): 867-78.
14. Sambrook and Russell editors. In Molecular Cloning: A Laboratory Manual 3 ed vol 1; CSHI Press. New York. 2007; 1.3.2-1.3.4.
15. Siu LK, Lu PL, Hsueh PR, Lin FM, Chang SC, Luh KT, et al. Bacteraemia due to extended spectrum β lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Pediatric Onchology ward: Clinical Feature and Identification of Different Plasmids carrying both SHV5 and TEM1 Genes. J Clin Microbiol. 1999; 37(12): 4020-07.
16. Oliveira CF, Salla A, Lara VM, Rieger A, Horta JA, Alves SH. Prevalence of extended spectrum β lactamase-producing micro-organism in nosocomial patients and molecular characterization of the SHV type isolates. Braz J Microbiol. 2010; 41(2): 1517.
17. Edelstein M, Pimkin M, Palagin I, Fdelsteini, Stratchounski. Prevalance and Molecular Epidemiology of CTX-M Extended Spectrum β lactamase producing Escherichia coli and Klebsiella pneumoniae in Russian Hospitals. Antimicrob Agents Chemother. 2003; 47(12): 3724-32.
18. Mathur P, Kapil A, Das B, Dhawan B. Prevalence of extended spectrum beta lactamase producing bacteria in a tertiary care hospital. Indian J Med Res. 2002; 115: 153-7.
19. Jones RN, Rhomberg PR, Varman DJ, Mathai D. A comparasion of antimicrobial activity of meropenem and selected broad-spectrum antimicrobials tested against multidrug resistant Gram negative bacilli including bacteriaemia Salmonella spp: Initial studies for MYSTIC programme in India. Int J Antimocrob Agents. 2002; 20: 426-31.
20. Yazdi M, Nazemi A, Mirinargasi M, Jafarpour M, Sharifi SH. Genotyping versus Phenotyping methods to detect Extended-Spectrum Beta-Lactamase (ESBLs) in uropathogenic Escherichia coli. Annals of Biological Research. 2012; 3(5): 2454-58.
21. Ravi SG, Namratha WN, Krishna BVS, Chandrashekar MR. Comparison of Disc Diffusion Methods for the Detection of Extended-Spectrum Beta LactamaseProducing Enterobacteriaceae. J Lab Physicians. 2011; 3(1): 33-06.
22. Yuan X, Jianfei C, Yuping H. Resistance of Strains Producing ExtendedSpectrum β-lactamase and Genotype Distribution among Escherichia coli in China. Pakistan J Zool. 2012; 44(2): 457-21.
23. Xiong Z, Zhu D, Wang F, Zhang Y, Okamoto R, Inoue M. Klebsiella pneumoniae producing three kinds of class A β lactamase encoded by one single plasmid isolated feom a patient in Huashan Hospital, Shanghai, China. Int J Antimicrobial Agents. 2002; 23: 262-07.
24. Welsh TR, Toleman MA, Jones RN. Comment on: Occurrence, prevalence and genetic environment of CTX-M β lactamase in Enterobacteriaceae from Indian hospitals. J Antimicrob Chemother. 2006; 58(6): 1260-03.
25. Dhillon RHP, Clark J. ESBL's: A clear and Present Dangers. Crit Care Res Pract. 2012.
26. Ye QH, Lau Y, Liang B, Tian SF. Antimicrobial resistance, genotypic charateristization and pulse field gel electrophoresis typing of extended spectrum β lactamase-producing clinical Escherichia coli strains in Macao, China. Chin Med J (Engl). 2011; 124(17): 2701-07.