A multiplex RT-PCR assay for rapid and differential diagnosis of four porcine diarrhea associated viruses in field samples from pig farms in East China from 2010 to 2012

Journal of Virological Methods

Volumn 194, Issue 1-2, 2013, Pages 107-112

  Zhao, Jin ^a   Shi, Bao jun ^a   Huang, Xiao guo ^b   Peng, Ming yi ^c   Zhang, Xiao min ^a   He, Dan ni ^a   Pang, Ran ^a   Zhou, Bin ^a   Chen, Pu yan ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b Changzhou Kangle Agricultur Co Ltd   (China)

^c Boehringer Ingelheim Animal Health Products Co Ltd   (China)

Author keywords

GAR; MRT PCR; PCV2; PEDV; Porcine diarrhea; TGEV

Indexed keywords

GENOMIC DNA; RNA;

ACCURACY; ANIMAL EXPERIMENT; ARTICLE; CHINA; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC TEST ACCURACY STUDY; DIARRHEA; DIFFERENTIAL DIAGNOSIS; EPIDEMIC; GENE AMPLIFICATION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PIG FARMING; PORCINE CIRCOVIRUS 2; PORCINE EPIDEMIC DIARRHEA VIRUS; PORCINE GROUP A ROTAVIRUS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRANSMISSIBLE GASTROENTERITIS VIRUS; VIRUS; VIRUS DETECTION; VIRUS GENE;

GAR; MRT-PCR; PCV2; PEDV; PORCINE DIARRHEA; TGEV;

ANIMALS; CHINA; CIRCOVIRUS; CORONAVIRIDAE; DIARRHEA; DNA PRIMERS; MULTIPLEX POLYMERASE CHAIN REACTION; PREVALENCE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA VIRUS INFECTIONS; RNA, VIRAL; ROTAVIRUS; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES;

EID: 84883809709     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.08.008     Document Type: Article

References (27)

1. Agüero M., Fernandez J., Romero L.J., Sanchez C., Arias M., Sanchez-Vizcaino J.M. A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and classical swine fever in clinical samples. Vet. Res. 2004, 35:551-563.
2. Chae C. Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. Vet. J. 2004, 68:41-49.
3. Chae C. Commercial porcine circovirus type 2 vaccines: efficacy and clinical application. Vet. J. 2012, 194:151-157.
4. Chamberlain J.S., Gibbs R.A., Ranier J.E., Nguyen P.N., Caskey C.T. Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res. 1988, 16:11141-11156.
5. Chen J., Wang C., Shi H., Qiu H., Liu S., Chen X., Zhang Z., Feng L. Molecular epidemiology of porcine epidemic diarrhea virus in China. Arch. Virol. 2010, 155:1471-1476.
6. Collins P.J., Martella V., Sleator R.D., Fanning S., O'Shea H. Detection and characterisation of group A rotavirus in asymptomatic piglets in southern Ireland. Arch. Virol. 2010, 155:1247-1259.
7. Díaz de Arce H., Pérez L.J., Frías M.T., Rosell R., Tarradas J., Núñez J.I., Ganges L. A multiplex RT-PCR assay for the rapid and differential diagnosis of classical swine fever and other pestivirus infections. Vet. Microbiol. 2009, 139:245-252.
8. Ellis J., Krakowka S., Lairmore M., Haines D., Bratanich A., Clark E., Allan G., Konoby C., Hassard L., Meehan B., Martin K., Harding J., Kennedy S., McNeilly F. Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. J. Vet. Diagn. Invest. 1999, 11:3-14.
9. Elschner M., Prudlo J., Hotzel H., Otto P., Sachse K. Nested reverse transcriptase polymerase chain reaction for the detection of group A rotavirus. J. Vet. Med. 2002, 49:77-81.
10. Ge F.F., Yang D.Q., Ju H.B., Wang J., Liu J., Liu P.H., Zhou J.P. Epidemiological survey of porcine epidemic diarrhea virus in swine farms in Shanghai, China. Arch. Virol. 2013, 2013 May 18 (Epub ahead of print).
11. Henegariu O., Heerema N.A., Dlouhy S.R., Vance G.H., Vogt P.H. Multiplex PCR: critical parameters and step-by-step protocol. Biotechniques 1997, 23:504-511.
12. Huang C., Hung J.J., Wu C.Y., Chien M.S. Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses. Vet. Microbiol. 2004, 101:209-214.
13. Jung K., Ha Y., Ha S.K., Kim J., Choi C., Park H.K., Kim S.H., Chae C. Identification of porcine circovirus type 2 in retrospective cases of pigs naturally infected with porcine epidemic diarrhoea virus. Vet. J. 2006, 171:166-168.
14. Jung K., Kim J., Ha Y., Choi C., Chae C. The efects of transplacental porcine circovirus type 2 infection on porcine epidemic diarrhoea virus-induced enteritis in preweaning piglets. Vet. J. 2006, 171:445-450.
15. Kim J., Ha Y., Jung K., Choi C., Chae C. Enteritis associated with porcine circovirus 2 in pigs. Can. J. Vet. Res. 2004, 68:218-221.
16. Kim S.Y., Song D.S., Park B.K. Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR. J. Vet. Diagn. Invest. 2001, 13:516-520.
17. Li W., Li H., Liu Y., Pan Y., Deng F., Song Y., Tang X., He Q. New variants of porcine epidemic diarrhea virus, China. Emerg. Infect. Dis. 2011, 18:1350-1353.
18. Meng F., Ren Y., Suo S., Sun X., Li X., Li P., Yang W., Li G., Li L., Schwegmann-Wessels C., Herrler G., Ren X. Evaluation on the efficacy and immunogenicity of recombinant DNA plasmids expressing spike genes from porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus. PLoS ONE 2013, 8:e57468.
19. Pan Y., Tian X., Li W., Zhou Q., Wang D., Bi Y., Chen F., Song Y. Isolation and characterization of a variant porcine epidemic diarrhea virus in China. Virol. J. 2012, 9:195.
20. Pensaert M.B., Yeo S. Porcine epidemic diarrhea. Diseases of Swine 2006, 367-372. Blackwell, Ames. 9th ed. B.E. Straw, J.J. Zimmerman, S. D'Allaire, D.J. Taylor (Eds.).
21. Rodák L., Valícek L., Smíd B., Nevoránková Z. ELISA optimized for porcine epidemic diarrhoea virus detection in faeces. Vet. Microbiol. 2005, 105:9-17.
22. Saif L., Sestak K. Transmissible gastroenteritis and porcine respiratory coronavirus. Diseases of Swine 2006, 578-582. Blackwell, Ames. 9th ed. B.E. Straw, J.J. Zimmerman, S. D'Allaire, D.J. Taylor (Eds.).
23. Sun R.Q., Cai R.J., Chen Y.Q., Liang P.S., Chen D.K., Song C.X. Outbreak of porcine epidemic diarrhea in suckling piglets, China. Emerg. Infect. Dis. 2012, 18:161-163.
24. Song D.S., Kang B.K., Oh J.S., Ha G.W., Yang J.S., Moon H.J., Jang Y.S., Park B.K. Multiplex reverse transcription-PCR for rapid differential detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine group A rotavirus. J. Vet. Diagn. Invest. 2006, 18:278-281.
25. Xu X.G., Chen G.D., Huang Y., Ding L., Li Z.C., Chang C.D., Wang C.Y., Tong D.W., Liu H.J. Development of multiplex PCR for simultaneous detection of six swine DNA and RNA viruses. J. Virol. Methods 2012, 183:69-74.
26. Yue F., Cui S., Zhang C., Yoon K.J. A multiplex PCR for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus in clinical specimens. Virus Genes 2009, 38:392-397.
27. Zhang Q., Hu R., Tang X., Wu C., He Q., Zhao Z., Chen H., Wu B. Occurrence and investigation of enteric viral infections in pigs with diarrhea in China. Arch. Virol. 2013, 158:1631-1636.