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Volumn , Issue 78, 2013, Pages

A quantitative assay to study protein: DNA interactions, discover transcriptional regulators of gene expression, and identify novel anti-tumor agents

Author keywords

Antibody; Cellular biology; DNA binding; Drug discovery; Drug screening; Enzyme linked immunosorbent assay (ELISA); Issue 78; Transcription factor; Transcription factors; Vitamin D

Indexed keywords


EID: 84883628313     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50512(2013)     Document Type: Article
Times cited : (13)

References (10)
  • 1
    • 34548169696 scopus 로고    scopus 로고
    • Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions
    • Hellman, L.M. & Fried, M.G. Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions. Nat. Protoc. 2, 1849-61 (2007).
    • (2007) Nat. Protoc. , vol.2 , pp. 1849-1861
    • Hellman, L.M.1    Fried, M.G.2
  • 2
    • 77953728203 scopus 로고    scopus 로고
    • High-throughput detection of nuclear factor-kappaB activity using a sensitive oligo-based chemiluminescent enzyme-linked immunosorbent assay
    • Bhattacharya, N., Sarno, A., Idler, I.S., et al. High-throughput detection of nuclear factor-kappaB activity using a sensitive oligo-based chemiluminescent enzyme-linked immunosorbent assay. Int. J. Cancer.127, 404-11 (2010).
    • (2010) Int. J. Cancer. , vol.127 , pp. 404-411
    • Bhattacharya, N.1    Sarno, A.2    Idler, I.S.3
  • 3
    • 70549087926 scopus 로고    scopus 로고
    • A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays
    • Hartzell, D.D., Trinklein, N.D., Mendez, J., et al. A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays. BMC Genomics. 10, 497 (2009).
    • (2009) BMC Genomics. , vol.10 , pp. 497
    • Hartzell, D.D.1    Trinklein, N.D.2    Mendez, J.3
  • 4
    • 71949088515 scopus 로고    scopus 로고
    • TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses
    • Vuori, K.A., Ahlskog, J.K., Sistonen, L., & Nikinmaa, M. TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses. FEBS J. 276, 7366-74 (2009).
    • (2009) FEBS J. , vol.276 , pp. 7366-7374
    • Vuori, K.A.1    Ahlskog, J.K.2    Sistonen, L.3    Nikinmaa, M.4
  • 5
    • 67650519130 scopus 로고    scopus 로고
    • Hyperglycemia Regulates RUNX2 Activation and Cellular Wound Healing through the Aldose Reductase Polyol Pathway
    • D'Souza, D.R., Salib, M.M., Bennett, J., et al. Hyperglycemia Regulates RUNX2 Activation and Cellular Wound Healing through the Aldose Reductase Polyol Pathway. J. Biol. Chem. 284, 17947-55 (2009).
    • (2009) J. Biol. Chem. , vol.284 , pp. 17947-17955
    • D'Souza, D.R.1    Salib, M.M.2    Bennett, J.3
  • 6
    • 84858734844 scopus 로고    scopus 로고
    • Regulation of RUNX2 transcription factor-DNA interactions and cell proliferation by Vitamin D3 (cholecalciferol) prohormone activity
    • Epub ahead of print Dec 2011
    • Underwood, K.F., D'Souza, D.R., Mochin, M.T., et al. Regulation of RUNX2 transcription factor-DNA interactions and cell proliferation by Vitamin D3 (cholecalciferol) prohormone activity. J. Bone Miner Res.27, 913-25 Epub ahead of print Dec 2011 (2012).
    • (2012) J. Bone Miner Res. , vol.27 , pp. 913-925
    • Underwood, K.F.1    D'Souza, D.R.2    Mochin, M.T.3
  • 7
    • 84883608880 scopus 로고    scopus 로고
    • Kinetic analysis of ß-galactosidase activity using the PowerWave HT and Gen5 data analysis software: Basic enzyme kinetic determinations
    • Held, P. Kinetic analysis of ß-galactosidase activity using the PowerWave HT and Gen5 data analysis software: basic enzyme kinetic determinations. Biotek Instruments Application Note. http://www.biotek.com/resources/docs/B-Gal_Michaelis-Menten_App_Note.pdf (2007).
    • (2007) Biotek Instruments Application Note.
    • Held, P.1
  • 8
    • 0035866185 scopus 로고    scopus 로고
    • Development of a sensitive multi-well colorimetric assay for active NFkappaB
    • Renard, P., Ernest, I., Houbion, A., et al. Development of a sensitive multi-well colorimetric assay for active NFkappaB. Nucleic Acids Res. 29, E21 (2001).
    • (2001) Nucleic Acids Res. , vol.29
    • Renard, P.1    Ernest, I.2    Houbion, A.3
  • 9
    • 67650682519 scopus 로고    scopus 로고
    • DNA topoisomerase I inhibitors: Chemistry, biology, and interfacial inhibition
    • Pommier, Y. DNA topoisomerase I inhibitors: chemistry, biology, and interfacial inhibition. Chem Rev.109, 2894-902 (2009).
    • (2009) Chem Rev. , vol.109 , pp. 2894-2902
    • Pommier, Y.1
  • 10
    • 84865812140 scopus 로고    scopus 로고
    • Genomics. ENCODE project writes eulogy for junk DNA
    • Pennisi, E. Genomics. ENCODE project writes eulogy for junk DNA. Science. 337, 1159-61 (2012).
    • (2012) Science. , vol.337 , pp. 1159-1161
    • Pennisi, E.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.