A NADPH-dependent (S)-imine reductase (SIR) from Streptomyces sp. GF3546 for asymmetric synthesis of optically active amines: Purification, characterization, gene cloning, and expression

Applied Microbiology and Biotechnology

Volumn 97, Issue 18, 2013, Pages 8079-8086

  Mitsukura, Koichi ^a   Kuramoto, Tatsuya ^a   Yoshida, Toyokazu ^a   Kimoto, Norihiro ^b   Yamamoto, Hiroaki ^b   Nagasawa, Toru ^a  

^a GIFU UNIVERSITY   (Japan)

^b DAICEL CORPORATION   (Japan)

Author keywords

(S) Imine reductase; Asymmetric reduction; Optically active amine; Streptomyces sp. GF3546

Indexed keywords

(S)-IMINE REDUCTASE; 6-PHOSPHOGLUCONATE DEHYDROGENASE; AMINO ACID SEQUENCE ANALYSIS; ASYMMETRIC REDUCTION; ENANTIOSELECTIVE REDUCTION; OPTICALLY ACTIVE; SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS; STREPTOMYCES SP;

AMINO ACIDS; BACTERIA; ELECTROPHORESIS; ESCHERICHIA COLI; PURIFICATION;

NITROGEN COMPOUNDS;

AMINE; CARBOXYLIC ACID DERIVATIVE; DIHYDROFOLIC ACID; DNA 16S; HOMODIMER; IMINE REDUCTASE; OXIDOREDUCTASE; PHOSPHOGLUCONATE DEHYDROGENASE; PYRROLINE DERIVATIVE; QUINUCLIDINE DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

AMINO ACID; CLONAL GROWTH; ELECTROKINESIS; ENZYME; ENZYME ACTIVITY; FILTRATION; GEL; GENE EXPRESSION; PROTEIN; REDUCTION;

AMINO ACID SEQUENCE; ARTICLE; ASYMMETRIC SYNTHESIS; BACILLUS CEREUS; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL ELECTROPHORESIS; GEL FILTRATION; GENE EXPRESSION; GENE SEQUENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; STREPTOMYCES;

ESCHERICHIA COLI; STREPTOMYCES SP.;

AMINES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; GENE EXPRESSION; IMINES; MOLECULAR SEQUENCE DATA; NADP; OXIDOREDUCTASES; SEQUENCE HOMOLOGY, AMINO ACID; SOIL MICROBIOLOGY; STREPTOMYCES; SUBSTRATE SPECIFICITY;

EID: 84883558751     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4629-4     Document Type: Article

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