Cloning and characterization of dihydrofolate reductases from deep-sea bacteria

Journal of Biochemistry

Volumn 147, Issue 4, 2010, Pages 591-599

  Murakami, Chiho ^a   Ohmae, Eiji ^a,b   Tate, Shin Ichi ^a   Gekko, Kunihiko ^a   Nakasone, Kaoru ^b   Kato, Chiaki ^c  

^a HIROSHIMA UNIVERSITY   (Japan)

^b KINKI UNIVERSITY   (Japan)

^c JAPAN AGENCY FOR MARINE EARTH SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Deep sea bacteria; Dihydrofolate reductase; Enzyme activity; High pressure adaptation; Molecular evolution

Indexed keywords

DIHYDROFOLATE REDUCTASE;

ACIDITY; ARTICLE; BACTERIUM; CELL CLONING; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; KINETICS; MORITELLA JAPONICA; MORITELLA YAYANOSII; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTOBACTERIUM PHOSPHOREUM; SHEWANELLA VIOLACEA; SPECTROFLUOROMETRY; TEMPERATURE; ULTRAVIOLET RADIATION;

ADAPTATION, BIOLOGICAL; AMINO ACID SEQUENCE; ATMOSPHERIC PRESSURE; BACTERIAL PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, BACTERIAL; GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MORITELLA; OCEANS AND SEAS; PHOTOBACTERIUM; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SEAWATER; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SHEWANELLA; TEMPERATURE; TETRAHYDROFOLATE DEHYDROGENASE;

ESCHERICHIA COLI; MORITELLA JAPONICA; MORITELLA YAYANOSII; PHOTOBACTERIUM PROFUNDUM; SHEWANELLA VIOLACEA;

EID: 77950611018     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvp206     Document Type: Article

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